A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

Guiqing Wen,1,2,* Qi Jing,1,2,* Aihui Liang,1,2 Zhiliang Jiang1,2 1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, China...

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Autores principales:	Wen G, Jing Q, Liang A, Jiang Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Graphene oxide nanoribbon catalysis immunoreaction SERS Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6373dcdb8c79400489579950adc74f34
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spelling	oai:doaj.org-article:6373dcdb8c79400489579950adc74f342021-12-02T03:24:29ZA new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis1178-2013https://doaj.org/article/6373dcdb8c79400489579950adc74f342018-10-01T00:00:00Zhttps://www.dovepress.com/a-new-sers-strategy-for-quantitative-analysis-of-trace-microalbuminuri-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Guiqing Wen,1,2,* Qi Jing,1,2,* Aihui Liang,1,2 Zhiliang Jiang1,2 1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, China; 2Department of Environment Science, College of Environment and Resources, Guangxi Normal University, Guilin, China *These authors contributed equally to this work Background: Microalbuminuria (mAlb) detection is essential for the diagnosis and prognosis of nephrotic patients and hypoproteinemia. In this article, we develop a new surface-enhanced Raman scattering (SERS) quantitative analysis method to detect mAlb in urine.Methods: Combined the mAlb immunoreaction with gold nanoreaction of graphene oxide nanoribbons (GONR)-HAuCl4-H2O2, and used Victoria blue B (VBB) as molecular probe with a SERS peak at 1,615 cm-1, a new SERS strategy for quantitative analysis of trace mAlb in urine was established.Results: The linear range of SERS quantitative analysis method is from 0.065 to 2.62 ng/mL, with a detection limit of 0.02 ng/mL. The SERS method was applied to analysis of mAlb in urine with good accuracy and reliability, the relative standard deviation is 0.49%–2.28% and the recovery is 96.9%–109.8%.Conclusion: This study demonstrated that the new SERS quantitative analysis method is of high sensitivity, good selectivity and simplicity. It has been applied to analysis of mAlb in urine, with satisfactory results. Keywords: graphene oxide nanoribbon, nanocatalysis, microalbumin immunoreaction, gold nanoreaction, SERSWen GJing QLiang AJiang ZDove Medical PressarticleGraphene oxide nanoribboncatalysisimmunoreactionSERSMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6099-6107 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene oxide nanoribbon catalysis immunoreaction SERS Medicine (General) R5-920
spellingShingle	Graphene oxide nanoribbon catalysis immunoreaction SERS Medicine (General) R5-920 Wen G Jing Q Liang A Jiang Z A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
description	Guiqing Wen,1,2,* Qi Jing,1,2,* Aihui Liang,1,2 Zhiliang Jiang1,2 1Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, China; 2Department of Environment Science, College of Environment and Resources, Guangxi Normal University, Guilin, China *These authors contributed equally to this work Background: Microalbuminuria (mAlb) detection is essential for the diagnosis and prognosis of nephrotic patients and hypoproteinemia. In this article, we develop a new surface-enhanced Raman scattering (SERS) quantitative analysis method to detect mAlb in urine.Methods: Combined the mAlb immunoreaction with gold nanoreaction of graphene oxide nanoribbons (GONR)-HAuCl4-H2O2, and used Victoria blue B (VBB) as molecular probe with a SERS peak at 1,615 cm-1, a new SERS strategy for quantitative analysis of trace mAlb in urine was established.Results: The linear range of SERS quantitative analysis method is from 0.065 to 2.62 ng/mL, with a detection limit of 0.02 ng/mL. The SERS method was applied to analysis of mAlb in urine with good accuracy and reliability, the relative standard deviation is 0.49%–2.28% and the recovery is 96.9%–109.8%.Conclusion: This study demonstrated that the new SERS quantitative analysis method is of high sensitivity, good selectivity and simplicity. It has been applied to analysis of mAlb in urine, with satisfactory results. Keywords: graphene oxide nanoribbon, nanocatalysis, microalbumin immunoreaction, gold nanoreaction, SERS
format	article
author	Wen G Jing Q Liang A Jiang Z
author_facet	Wen G Jing Q Liang A Jiang Z
author_sort	Wen G
title	A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
title_short	A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
title_full	A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
title_fullStr	A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
title_full_unstemmed	A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
title_sort	new sers strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/6373dcdb8c79400489579950adc74f34
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A new SERS strategy for quantitative analysis of trace microalbuminuria based on immunorecognition and graphene oxide nanoribbon catalysis

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