Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression

Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression

Wa Cai,1,2 Wen Ma,1 Guan-Tao Wang,1 Yi-Jing Li,1 Wei-Dong Shen1,21Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Department of Acupuncture, Shanghai Shuguang Hospi...

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Autores principales: Cai W, Ma W, Wang GT, Li YJ, Shen WD
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2019
Materias:
electroacupuncture
post stroke depression
sonic hedgehog signaling pathway
oxidative stress
inflammation
5-HT;
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
Acceso en línea:https://doaj.org/article/6381ebd11804483eaa0eace1fe28e089
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spelling oai:doaj.org-article:6381ebd11804483eaa0eace1fe28e0892021-12-02T05:57:09ZAntidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression1178-2021https://doaj.org/article/6381ebd11804483eaa0eace1fe28e0892019-05-01T00:00:00Zhttps://www.dovepress.com/antidepressant-anti-inflammatory-and-antioxidant-effects-of-electroacu-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Wa Cai,1,2 Wen Ma,1 Guan-Tao Wang,1 Yi-Jing Li,1 Wei-Dong Shen1,21Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Department of Acupuncture, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of ChinaBackground: Poststroke depression (PSD) is the most frequent psychological sequela after stroke. Electroacupuncture (EA) treatment is effective for PSD. The study aimed at clarifying the mechanisms of EA’s antidepressant effects in a PSD rat model.Methods: We used middle cerebral artery occlusion to establish the rat model of PSD. Tests of sucrose preference and locomotor activity were performed to examine depressive-like behaviors. We measured malondialdehyde, GSH, SOD, IL6, IL1β, TNFα, and 5HT with ELISA. The hippocampal Shh-signaling pathway was assessed by Western blot.Results: EA significantly decreased sucrose preference and locomotor activities of PSD rats, reduced IL6, TNFα, increased GSH, and upregulated 5HT, and also slightly reduced IL1β and malondialdehyde, all of which were measured with ELISA. The Shh-signaling pathway assessed by Western blotting was activated by EA. Those changes were inhibited by the Shh-pathway inhibitor cyclopamine.Conclusion: EA effectively alleviated depressive-like behaviors in PSD by suppressing inflammation and oxidative stress through activation of the Shh-signaling pathway.Keywords: electroacupuncture, poststroke depression, sonic hedgehog–signaling pathway, oxidative stress, inflammation, 5HTCai WMa WWang GTLi YJShen WDDove Medical Pressarticleelectroacupuncturepost stroke depressionsonic hedgehog signaling pathwayoxidative stressinflammation5-HT;Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 1403-1411 (2019)
institution DOAJ
collection DOAJ
language EN
topic electroacupuncture
post stroke depression
sonic hedgehog signaling pathway
oxidative stress
inflammation
5-HT;
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
spellingShingle electroacupuncture
post stroke depression
sonic hedgehog signaling pathway
oxidative stress
inflammation
5-HT;
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
Cai W
Ma W
Wang GT
Li YJ
Shen WD
Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
description Wa Cai,1,2 Wen Ma,1 Guan-Tao Wang,1 Yi-Jing Li,1 Wei-Dong Shen1,21Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Department of Acupuncture, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of ChinaBackground: Poststroke depression (PSD) is the most frequent psychological sequela after stroke. Electroacupuncture (EA) treatment is effective for PSD. The study aimed at clarifying the mechanisms of EA’s antidepressant effects in a PSD rat model.Methods: We used middle cerebral artery occlusion to establish the rat model of PSD. Tests of sucrose preference and locomotor activity were performed to examine depressive-like behaviors. We measured malondialdehyde, GSH, SOD, IL6, IL1β, TNFα, and 5HT with ELISA. The hippocampal Shh-signaling pathway was assessed by Western blot.Results: EA significantly decreased sucrose preference and locomotor activities of PSD rats, reduced IL6, TNFα, increased GSH, and upregulated 5HT, and also slightly reduced IL1β and malondialdehyde, all of which were measured with ELISA. The Shh-signaling pathway assessed by Western blotting was activated by EA. Those changes were inhibited by the Shh-pathway inhibitor cyclopamine.Conclusion: EA effectively alleviated depressive-like behaviors in PSD by suppressing inflammation and oxidative stress through activation of the Shh-signaling pathway.Keywords: electroacupuncture, poststroke depression, sonic hedgehog–signaling pathway, oxidative stress, inflammation, 5HT
format article
author Cai W
Ma W
Wang GT
Li YJ
Shen WD
author_facet Cai W
Ma W
Wang GT
Li YJ
Shen WD
author_sort Cai W
title Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
title_short Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
title_full Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
title_fullStr Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
title_full_unstemmed Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
title_sort antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression
publisher Dove Medical Press
publishDate 2019
url https://doaj.org/article/6381ebd11804483eaa0eace1fe28e089
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