Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.

Protein phosphorylation is a dynamic and reversible event that greatly influences cellular function. Identifying the key regulatory elements that determine cellular phenotypes during development and oncogenesis requires the ability to dynamically monitor proteome-wide events. Here, we report the dev...

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Autores principales: Crystal L Woodard, C Rory Goodwin, Jun Wan, Shuli Xia, Robert Newman, Jianfei Hu, Jin Zhang, S Diane Hayward, Jiang Qian, John Laterra, Heng Zhu
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/638242fc6a6f4262a2da615d0a3467af
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spelling oai:doaj.org-article:638242fc6a6f4262a2da615d0a3467af2021-11-18T08:57:25ZProfiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.1932-620310.1371/journal.pone.0072671https://doaj.org/article/638242fc6a6f4262a2da615d0a3467af2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023761/?tool=EBIhttps://doaj.org/toc/1932-6203Protein phosphorylation is a dynamic and reversible event that greatly influences cellular function. Identifying the key regulatory elements that determine cellular phenotypes during development and oncogenesis requires the ability to dynamically monitor proteome-wide events. Here, we report the development of a new strategy to monitor dynamic changes of protein phosphorylation in cells and tissues using functional protein microarrays as the readout. To demonstrate this technology's ability to identify condition-dependent phosphorylation events, human protein microarrays were incubated with lysates from cells or tissues under activation or inhibition of c-Met, a receptor tyrosine kinase involved in tissue morphogenesis and malignancy. By comparing the differences between the protein phosphorylation profiles obtained using the protein microarrays, we were able to recover many of the proteins that are known to be specifically activated (i.e., phosphorylated) upon c-Met activation by the hepatocyte growth factor (HGF). Most importantly, we discovered many proteins that were differentially phosphorylated by lysates from cells or tissues when the c-Met pathway was active. Using phosphorylation-specific antibodies, we were able to validate several candidate proteins as new downstream components of the c-Met signaling pathway in cells. We envision that this new approach, like its DNA microarray counterpart, can be further extended toward profiling dynamics of global protein phosphorylation under many different physiological conditions both in cellulo and in vivo in a high-throughput and cost-effective fashion.Crystal L WoodardC Rory GoodwinJun WanShuli XiaRobert NewmanJianfei HuJin ZhangS Diane HaywardJiang QianJohn LaterraHeng ZhuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72671 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Crystal L Woodard
C Rory Goodwin
Jun Wan
Shuli Xia
Robert Newman
Jianfei Hu
Jin Zhang
S Diane Hayward
Jiang Qian
John Laterra
Heng Zhu
Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
description Protein phosphorylation is a dynamic and reversible event that greatly influences cellular function. Identifying the key regulatory elements that determine cellular phenotypes during development and oncogenesis requires the ability to dynamically monitor proteome-wide events. Here, we report the development of a new strategy to monitor dynamic changes of protein phosphorylation in cells and tissues using functional protein microarrays as the readout. To demonstrate this technology's ability to identify condition-dependent phosphorylation events, human protein microarrays were incubated with lysates from cells or tissues under activation or inhibition of c-Met, a receptor tyrosine kinase involved in tissue morphogenesis and malignancy. By comparing the differences between the protein phosphorylation profiles obtained using the protein microarrays, we were able to recover many of the proteins that are known to be specifically activated (i.e., phosphorylated) upon c-Met activation by the hepatocyte growth factor (HGF). Most importantly, we discovered many proteins that were differentially phosphorylated by lysates from cells or tissues when the c-Met pathway was active. Using phosphorylation-specific antibodies, we were able to validate several candidate proteins as new downstream components of the c-Met signaling pathway in cells. We envision that this new approach, like its DNA microarray counterpart, can be further extended toward profiling dynamics of global protein phosphorylation under many different physiological conditions both in cellulo and in vivo in a high-throughput and cost-effective fashion.
format article
author Crystal L Woodard
C Rory Goodwin
Jun Wan
Shuli Xia
Robert Newman
Jianfei Hu
Jin Zhang
S Diane Hayward
Jiang Qian
John Laterra
Heng Zhu
author_facet Crystal L Woodard
C Rory Goodwin
Jun Wan
Shuli Xia
Robert Newman
Jianfei Hu
Jin Zhang
S Diane Hayward
Jiang Qian
John Laterra
Heng Zhu
author_sort Crystal L Woodard
title Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
title_short Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
title_full Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
title_fullStr Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
title_full_unstemmed Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling.
title_sort profiling the dynamics of a human phosphorylome reveals new components in hgf/c-met signaling.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/638242fc6a6f4262a2da615d0a3467af
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