The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, P...

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Autores principales: Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L
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Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/638ce463bd594ef7b3a832256cc1e394
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spelling oai:doaj.org-article:638ce463bd594ef7b3a832256cc1e3942021-12-02T04:33:05ZThe diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells1178-2013https://doaj.org/article/638ce463bd594ef7b3a832256cc1e3942015-11-01T00:00:00Zhttps://www.dovepress.com/the-diameter-of-nanotubes-formed-on-ti-6al-4v-alloy-controls-the-adhes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic Abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties. Keywords: nanostructure, titanium nanotubes, cell adhesion, Saos-2 cells, osteogenic differentiationFilova EFojt JKryslova MMoravec HJoska LBacakova LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7145-7163 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Filova E
Fojt J
Kryslova M
Moravec H
Joska L
Bacakova L
The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
description Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1 1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic Abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties. Keywords: nanostructure, titanium nanotubes, cell adhesion, Saos-2 cells, osteogenic differentiation
format article
author Filova E
Fojt J
Kryslova M
Moravec H
Joska L
Bacakova L
author_facet Filova E
Fojt J
Kryslova M
Moravec H
Joska L
Bacakova L
author_sort Filova E
title The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
title_short The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
title_full The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
title_fullStr The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
title_full_unstemmed The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells
title_sort diameter of nanotubes formed on ti-6al-4v alloy controls the adhesion and differentiation of saos-2 cells
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/638ce463bd594ef7b3a832256cc1e394
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