Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease

Abstract The association between the gut microbiota and the development of lupus is unclear. We investigated alterations in the gut microbiota after induction of lupus in a murine model using viral peptide of human cytomegalovirus (HCMV). Three treatment arms for the animals were prepared: intraperi...

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Autores principales:	Yen-Fu Chen, Ao-Ho Hsieh, Lian-Chin Wang, Yun-Ju Huang, Yun-Chen Tsai, Wen-Yi Tseng, Yu-Lun Kuo, Shue-Fen Luo, Kuang-Hui Yu, Chang-Fu Kuo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63945577a2744e92a34b76bbcd50c2dd
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spelling	oai:doaj.org-article:63945577a2744e92a34b76bbcd50c2dd2021-11-28T12:19:47ZFecal microbiota changes in NZB/W F1 mice after induction of lupus disease10.1038/s41598-021-02422-92045-2322https://doaj.org/article/63945577a2744e92a34b76bbcd50c2dd2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02422-9https://doaj.org/toc/2045-2322Abstract The association between the gut microbiota and the development of lupus is unclear. We investigated alterations in the gut microbiota after induction of lupus in a murine model using viral peptide of human cytomegalovirus (HCMV). Three treatment arms for the animals were prepared: intraperitoneal injection of HCMVpp65 peptide, adjuvant alone, and PBS injection. Feces were collected before and after lupus induction biweekly for 16S rRNA sequencing. HCMVpp65 peptide immunization induced lupus-like effects, with higher levels of anti-dsDNA antibodies, creatinine, proteinuria, and glomerular damage, compared with mice treated with nothing or adjuvant only. The Simpson diversity value was higher in mice injected with HCMVpp65 peptide, but there was no difference in ACE or Chao1 among the three groups. Statistical analysis of metagenomic profiles showed a higher abundance of various families (Saccharimonadaceae, Marinifiaceae, and Desulfovibrionaceae) and genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) in HCMVpp65 peptide-treated mice. Significant correlations between increased abundances of related genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) and HCMVpp65 peptide immunization-induced lupus-like effects were observed. This study provides insight into the changes in the gut microbiota after lupus onset in a murine model.Yen-Fu ChenAo-Ho HsiehLian-Chin WangYun-Ju HuangYun-Chen TsaiWen-Yi TsengYu-Lun KuoShue-Fen LuoKuang-Hui YuChang-Fu KuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yen-Fu Chen Ao-Ho Hsieh Lian-Chin Wang Yun-Ju Huang Yun-Chen Tsai Wen-Yi Tseng Yu-Lun Kuo Shue-Fen Luo Kuang-Hui Yu Chang-Fu Kuo Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
description	Abstract The association between the gut microbiota and the development of lupus is unclear. We investigated alterations in the gut microbiota after induction of lupus in a murine model using viral peptide of human cytomegalovirus (HCMV). Three treatment arms for the animals were prepared: intraperitoneal injection of HCMVpp65 peptide, adjuvant alone, and PBS injection. Feces were collected before and after lupus induction biweekly for 16S rRNA sequencing. HCMVpp65 peptide immunization induced lupus-like effects, with higher levels of anti-dsDNA antibodies, creatinine, proteinuria, and glomerular damage, compared with mice treated with nothing or adjuvant only. The Simpson diversity value was higher in mice injected with HCMVpp65 peptide, but there was no difference in ACE or Chao1 among the three groups. Statistical analysis of metagenomic profiles showed a higher abundance of various families (Saccharimonadaceae, Marinifiaceae, and Desulfovibrionaceae) and genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) in HCMVpp65 peptide-treated mice. Significant correlations between increased abundances of related genera (Candidatus Saccharimonas, Roseburia, Odoribacter, and Desulfovibrio) and HCMVpp65 peptide immunization-induced lupus-like effects were observed. This study provides insight into the changes in the gut microbiota after lupus onset in a murine model.
format	article
author	Yen-Fu Chen Ao-Ho Hsieh Lian-Chin Wang Yun-Ju Huang Yun-Chen Tsai Wen-Yi Tseng Yu-Lun Kuo Shue-Fen Luo Kuang-Hui Yu Chang-Fu Kuo
author_facet	Yen-Fu Chen Ao-Ho Hsieh Lian-Chin Wang Yun-Ju Huang Yun-Chen Tsai Wen-Yi Tseng Yu-Lun Kuo Shue-Fen Luo Kuang-Hui Yu Chang-Fu Kuo
author_sort	Yen-Fu Chen
title	Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
title_short	Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
title_full	Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
title_fullStr	Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
title_full_unstemmed	Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease
title_sort	fecal microbiota changes in nzb/w f1 mice after induction of lupus disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63945577a2744e92a34b76bbcd50c2dd
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_version_	1718408018650988544

Fecal microbiota changes in NZB/W F1 mice after induction of lupus disease

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