Immunochromatographic strip for rapid detection of phenylethanolamine A

A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of det...

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Autores principales:	Wei Jiang, Lu Zeng, Liqiang Liu, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	phenylethanolamine a immunochromatographic strip antibody swine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/639d0ee697d34fec9a72759509592492
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spelling	oai:doaj.org-article:639d0ee697d34fec9a727595095924922021-11-26T11:19:46ZImmunochromatographic strip for rapid detection of phenylethanolamine A0954-01051465-344310.1080/09540105.2017.1364709https://doaj.org/article/639d0ee697d34fec9a727595095924922018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1364709https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.Wei JiangLu ZengLiqiang LiuShanshan SongHua KuangTaylor & Francis Grouparticlephenylethanolamine aimmunochromatographic stripantibodyswineAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 182-192 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	phenylethanolamine a immunochromatographic strip antibody swine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	phenylethanolamine a immunochromatographic strip antibody swine Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang Immunochromatographic strip for rapid detection of phenylethanolamine A
description	A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.
format	article
author	Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang
author_facet	Wei Jiang Lu Zeng Liqiang Liu Shanshan Song Hua Kuang
author_sort	Wei Jiang
title	Immunochromatographic strip for rapid detection of phenylethanolamine A
title_short	Immunochromatographic strip for rapid detection of phenylethanolamine A
title_full	Immunochromatographic strip for rapid detection of phenylethanolamine A
title_fullStr	Immunochromatographic strip for rapid detection of phenylethanolamine A
title_full_unstemmed	Immunochromatographic strip for rapid detection of phenylethanolamine A
title_sort	immunochromatographic strip for rapid detection of phenylethanolamine a
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/639d0ee697d34fec9a72759509592492
work_keys_str_mv	AT weijiang immunochromatographicstripforrapiddetectionofphenylethanolaminea AT luzeng immunochromatographicstripforrapiddetectionofphenylethanolaminea AT liqiangliu immunochromatographicstripforrapiddetectionofphenylethanolaminea AT shanshansong immunochromatographicstripforrapiddetectionofphenylethanolaminea AT huakuang immunochromatographicstripforrapiddetectionofphenylethanolaminea
_version_	1718409537893957632

Immunochromatographic strip for rapid detection of phenylethanolamine A

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