Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma

Abstract Background Three-dimensional (3D) cell culture methods are identified for simulating the biological microenvironment and demonstrating more similarity to in vivo circumstances. Anaplastic thyroid carcinoma (ATC) is a lethal endocrine malignancy. Despite different treatment approaches, no im...

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Autores principales:	Hilda Samimi, Alireza Naderi Sohi, Shiva Irani, Ehsan Arefian, Mojdeh Mahdiannasser, Parviz Fallah, Vahid Haghpanah
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Anaplastic thyroid carcinoma Two-dimensional cell culture Three-dimensional cell culture Anticancer drug Half-maximal inhibitory concentration Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/63a0f3e0635049c38e577fbd611fefb3
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spelling	oai:doaj.org-article:63a0f3e0635049c38e577fbd611fefb32021-12-05T12:05:22ZAlginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma10.1186/s13044-021-00118-w1756-6614https://doaj.org/article/63a0f3e0635049c38e577fbd611fefb32021-12-01T00:00:00Zhttps://doi.org/10.1186/s13044-021-00118-whttps://doaj.org/toc/1756-6614Abstract Background Three-dimensional (3D) cell culture methods are identified for simulating the biological microenvironment and demonstrating more similarity to in vivo circumstances. Anaplastic thyroid carcinoma (ATC) is a lethal endocrine malignancy. Despite different treatment approaches, no improvement in the survival rate of the patients has been shown. In this study, we used the 3D in vitro ATC model to investigate the cytotoxic effect of BI-847325 anticancer drug in two-dimensional (2D)- and 3D- cultured cells. Methods Human ATC cell lines, C643 and SW1736, were cultured in one percentage (w/v) sodium alginate. Spheroids were incubated in medium for one week. The reproducibility of the fabrication of alginate beads was evaluated. Encapsulation of the cells in alginate was examined by DAPI (4′,6-diamidino-2-phenylindole) staining. Survival of alginate-encapsulated cells was evaluated by CFSE (5,6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining. The population doubling times of C643 and SW1736 cell lines cultured in 2D monolayer as well as in 3D system were calculated. The cytotoxic effect of BI-847325 on 2D- and 3D- cultured cell lines was assessed for 24–72 h by MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay. Finally, the 3D culture results were compared with the 2D culture method. Results The half-maximal inhibitory concentration (IC50) values of BI-847325 were higher in 3D culture compared to 2D culture. The cytotoxicity data indicated that 3D in vitro models were more resistant to chemotherapy agents. Conclusions The findings of this study are beneficial for developing in vitro ATC 3D models to analyze the efficacy of different chemotherapy drugs and formulations.Hilda SamimiAlireza Naderi SohiShiva IraniEhsan ArefianMojdeh MahdiannasserParviz FallahVahid HaghpanahBMCarticleAnaplastic thyroid carcinomaTwo-dimensional cell cultureThree-dimensional cell cultureAnticancer drugHalf-maximal inhibitory concentrationDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENThyroid Research, Vol 14, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anaplastic thyroid carcinoma Two-dimensional cell culture Three-dimensional cell culture Anticancer drug Half-maximal inhibitory concentration Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	Anaplastic thyroid carcinoma Two-dimensional cell culture Three-dimensional cell culture Anticancer drug Half-maximal inhibitory concentration Diseases of the endocrine glands. Clinical endocrinology RC648-665 Hilda Samimi Alireza Naderi Sohi Shiva Irani Ehsan Arefian Mojdeh Mahdiannasser Parviz Fallah Vahid Haghpanah Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
description	Abstract Background Three-dimensional (3D) cell culture methods are identified for simulating the biological microenvironment and demonstrating more similarity to in vivo circumstances. Anaplastic thyroid carcinoma (ATC) is a lethal endocrine malignancy. Despite different treatment approaches, no improvement in the survival rate of the patients has been shown. In this study, we used the 3D in vitro ATC model to investigate the cytotoxic effect of BI-847325 anticancer drug in two-dimensional (2D)- and 3D- cultured cells. Methods Human ATC cell lines, C643 and SW1736, were cultured in one percentage (w/v) sodium alginate. Spheroids were incubated in medium for one week. The reproducibility of the fabrication of alginate beads was evaluated. Encapsulation of the cells in alginate was examined by DAPI (4′,6-diamidino-2-phenylindole) staining. Survival of alginate-encapsulated cells was evaluated by CFSE (5,6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining. The population doubling times of C643 and SW1736 cell lines cultured in 2D monolayer as well as in 3D system were calculated. The cytotoxic effect of BI-847325 on 2D- and 3D- cultured cell lines was assessed for 24–72 h by MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay. Finally, the 3D culture results were compared with the 2D culture method. Results The half-maximal inhibitory concentration (IC50) values of BI-847325 were higher in 3D culture compared to 2D culture. The cytotoxicity data indicated that 3D in vitro models were more resistant to chemotherapy agents. Conclusions The findings of this study are beneficial for developing in vitro ATC 3D models to analyze the efficacy of different chemotherapy drugs and formulations.
format	article
author	Hilda Samimi Alireza Naderi Sohi Shiva Irani Ehsan Arefian Mojdeh Mahdiannasser Parviz Fallah Vahid Haghpanah
author_facet	Hilda Samimi Alireza Naderi Sohi Shiva Irani Ehsan Arefian Mojdeh Mahdiannasser Parviz Fallah Vahid Haghpanah
author_sort	Hilda Samimi
title	Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
title_short	Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
title_full	Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
title_fullStr	Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
title_full_unstemmed	Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
title_sort	alginate-based 3d cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/63a0f3e0635049c38e577fbd611fefb3
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Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma

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