Immunohistochemical investigation of rat cardiac muscle in experimental ischemia

Ischemic heart disease is currently the most common cause of heart failure development. Nevertheless, morphological and immunohistochemical changes in myocardium in early stages of ischemic heart disease require further studies. The aim was to study redistribution of immunohistochemical markers...

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Autores principales:	B.A. Nadraga, Kh.I. Strus, A.M. Yashchenko, A.D. Lutsyk
Formato:	article
Lenguaje:	EN UK
Publicado:	Danylo Halytsky Lviv National Medical University 2020
Materias:	rats, myocardial acute experimental ischemia, immunohistochemistry Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/63a13fbd9744445ebdceff1cd13df4aa
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spelling	oai:doaj.org-article:63a13fbd9744445ebdceff1cd13df4aa2021-11-10T19:49:51ZImmunohistochemical investigation of rat cardiac muscle in experimental ischemia1029-42442415-330310.25040/aml2020.01.011https://doaj.org/article/63a13fbd9744445ebdceff1cd13df4aa2020-02-01T00:00:00Zhttps://amljournal.com/index.php/journal/article/view/33https://doaj.org/toc/1029-4244https://doaj.org/toc/2415-3303Ischemic heart disease is currently the most common cause of heart failure development. Nevertheless, morphological and immunohistochemical changes in myocardium in early stages of ischemic heart disease require further studies. The aim was to study redistribution of immunohistochemical markers in structural components of myocardium in early stages of ischemia development. Material and methods. The study was carried out on 20 adult male Wistar rats weighing 180-220 g (5 control and 15 experimental animals). Myocardial ischemia was induced by subcutaneous administration of a 0.1% solution of adrenaline (epinephrine hydrotartrate) at a dose of 0.2 ml per 100 g of body weight. Forty-eight hours after the adrenaline injection, the rats were euthanized by ethyl ether overdose. Samples of the heart muscle excised from the atria and the ventricles were fixed in 4% neutral formalin and embedded in paraffin. Immunohistochemical studies were performed using primary monoclonal antibodies to Ki-67, VEGF, CD34, and polyclonal Casp3. The reaction products were visualized using 3,3'-diaminobenzidine tetrahydrochloride as chromogen. Results and discussion. Acute ischemia induced apoptotic processes in the cardiomyocytes accompanied by destruction of myofibrils with the accumulation of damaged myofibrillary proteins in the form of cytoplasmatic granularity; these changes apparently led to impaired cardiomyocyte contractility. The above changes were combined with increased immunoreactivity of vascular bed endothelium, and emergence of VEGF-positive macrophages in perivascular spaces. The mast cells in both groups of rats showed Casp3/Кі-67-reactivity. Conclusion. The rat heart myocardium, subjected to experimental adrenalin-induced ischemia, demonstrated significant changes in microcirculatory bed endothelial cells immunoreactivity, which was accompanied by fine-grained dystrophy of contractile cardiomyocytes myofibrillary content, both signs including impairments of these cells functional activities.B.A. NadragaKh.I. StrusA.M. YashchenkoA.D. LutsykDanylo Halytsky Lviv National Medical Universityarticlerats, myocardial acute experimental ischemia, immunohistochemistryMedicine (General)R5-920ENUKActa Medica Leopoliensia, Vol 26, Iss 1, Pp 11-19 (2020)
institution	DOAJ
collection	DOAJ
language	EN UK
topic	rats, myocardial acute experimental ischemia, immunohistochemistry Medicine (General) R5-920
spellingShingle	rats, myocardial acute experimental ischemia, immunohistochemistry Medicine (General) R5-920 B.A. Nadraga Kh.I. Strus A.M. Yashchenko A.D. Lutsyk Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
description	Ischemic heart disease is currently the most common cause of heart failure development. Nevertheless, morphological and immunohistochemical changes in myocardium in early stages of ischemic heart disease require further studies. The aim was to study redistribution of immunohistochemical markers in structural components of myocardium in early stages of ischemia development. Material and methods. The study was carried out on 20 adult male Wistar rats weighing 180-220 g (5 control and 15 experimental animals). Myocardial ischemia was induced by subcutaneous administration of a 0.1% solution of adrenaline (epinephrine hydrotartrate) at a dose of 0.2 ml per 100 g of body weight. Forty-eight hours after the adrenaline injection, the rats were euthanized by ethyl ether overdose. Samples of the heart muscle excised from the atria and the ventricles were fixed in 4% neutral formalin and embedded in paraffin. Immunohistochemical studies were performed using primary monoclonal antibodies to Ki-67, VEGF, CD34, and polyclonal Casp3. The reaction products were visualized using 3,3'-diaminobenzidine tetrahydrochloride as chromogen. Results and discussion. Acute ischemia induced apoptotic processes in the cardiomyocytes accompanied by destruction of myofibrils with the accumulation of damaged myofibrillary proteins in the form of cytoplasmatic granularity; these changes apparently led to impaired cardiomyocyte contractility. The above changes were combined with increased immunoreactivity of vascular bed endothelium, and emergence of VEGF-positive macrophages in perivascular spaces. The mast cells in both groups of rats showed Casp3/Кі-67-reactivity. Conclusion. The rat heart myocardium, subjected to experimental adrenalin-induced ischemia, demonstrated significant changes in microcirculatory bed endothelial cells immunoreactivity, which was accompanied by fine-grained dystrophy of contractile cardiomyocytes myofibrillary content, both signs including impairments of these cells functional activities.
format	article
author	B.A. Nadraga Kh.I. Strus A.M. Yashchenko A.D. Lutsyk
author_facet	B.A. Nadraga Kh.I. Strus A.M. Yashchenko A.D. Lutsyk
author_sort	B.A. Nadraga
title	Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
title_short	Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
title_full	Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
title_fullStr	Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
title_full_unstemmed	Immunohistochemical investigation of rat cardiac muscle in experimental ischemia
title_sort	immunohistochemical investigation of rat cardiac muscle in experimental ischemia
publisher	Danylo Halytsky Lviv National Medical University
publishDate	2020
url	https://doaj.org/article/63a13fbd9744445ebdceff1cd13df4aa
work_keys_str_mv	AT banadraga immunohistochemicalinvestigationofratcardiacmuscleinexperimentalischemia AT khistrus immunohistochemicalinvestigationofratcardiacmuscleinexperimentalischemia AT amyashchenko immunohistochemicalinvestigationofratcardiacmuscleinexperimentalischemia AT adlutsyk immunohistochemicalinvestigationofratcardiacmuscleinexperimentalischemia
_version_	1718439782567116800

Immunohistochemical investigation of rat cardiac muscle in experimental ischemia

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