Authentication of Zingiber Species Based on Analysis of Metabolite Profiles

Zingiber corallinum and Zingiber montanum, which belong to the Zingiberaceae family, are traditional Chinese folk medicinal herbs in Guizhou and Yunnan Province of China. They share great similarities in morphology, chemical constituent, and DNA barcoding sequence. The taxonomy of the two Zingiber s...

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Autores principales:	Chenxi Wang, Yue Zhang, Hui Ding, Meifang Song, Jiaxin Yin, Heshui Yu, Zheng Li, Lifeng Han, Zhonglian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Zingiber chloroplast genome untargeted metabolomics gas chromatography-mass spectrometry chemometric methods Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/63a2206568d24d509d6db5a5413a6a34
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id	oai:doaj.org-article:63a2206568d24d509d6db5a5413a6a34
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spelling	oai:doaj.org-article:63a2206568d24d509d6db5a5413a6a342021-11-30T10:08:59ZAuthentication of Zingiber Species Based on Analysis of Metabolite Profiles1664-462X10.3389/fpls.2021.705446https://doaj.org/article/63a2206568d24d509d6db5a5413a6a342021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.705446/fullhttps://doaj.org/toc/1664-462XZingiber corallinum and Zingiber montanum, which belong to the Zingiberaceae family, are traditional Chinese folk medicinal herbs in Guizhou and Yunnan Province of China. They share great similarities in morphology, chemical constituent, and DNA barcoding sequence. The taxonomy of the two Zingiber species is controversial and discrimination of traditional Chinese medicines directly affects the pharmacological and clinical effects. In the present study, we performed a systemic analysis of “super-barcode” and untargeted metabolomics between Z. corallinum and Z. montanum using chloroplast (cp) genome sequencing and gas chromatography-mass spectrometry (GC-MS) analysis. Comparison and phylogenetic analysis of cp genomes of the two Zingiber species showed that the cp genome could not guarantee the accuracy of identification. An untargeted metabolomics strategy combining GC-MS with chemometric methods was proposed to distinguish the Zingiber samples of known variety. A total of 51 volatile compounds extracted from Z. corallinum and Z. montanum were identified, and nine compounds were selected as candidate metabolic markers to reveal the significant difference between Z. corallinum and Z. montanum. The performance of the untargeted metabolomic approach was verified with unknown Zingiber samples. Although the cp genomes could not be used to identify Zingiber species in this study, it will still provide a valuable genomics resource for population studies in the Zingiberaceae family, and the GC-MS based metabolic fingerprint is more promising for species identification and safe application of Z. corallinum and Z. montanum.Chenxi WangYue ZhangHui DingMeifang SongJiaxin YinHeshui YuZheng LiLifeng HanZhonglian ZhangFrontiers Media S.A.articleZingiberchloroplast genomeuntargeted metabolomicsgas chromatography-mass spectrometrychemometric methodsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zingiber chloroplast genome untargeted metabolomics gas chromatography-mass spectrometry chemometric methods Plant culture SB1-1110
spellingShingle	Zingiber chloroplast genome untargeted metabolomics gas chromatography-mass spectrometry chemometric methods Plant culture SB1-1110 Chenxi Wang Yue Zhang Hui Ding Meifang Song Jiaxin Yin Heshui Yu Zheng Li Lifeng Han Zhonglian Zhang Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
description	Zingiber corallinum and Zingiber montanum, which belong to the Zingiberaceae family, are traditional Chinese folk medicinal herbs in Guizhou and Yunnan Province of China. They share great similarities in morphology, chemical constituent, and DNA barcoding sequence. The taxonomy of the two Zingiber species is controversial and discrimination of traditional Chinese medicines directly affects the pharmacological and clinical effects. In the present study, we performed a systemic analysis of “super-barcode” and untargeted metabolomics between Z. corallinum and Z. montanum using chloroplast (cp) genome sequencing and gas chromatography-mass spectrometry (GC-MS) analysis. Comparison and phylogenetic analysis of cp genomes of the two Zingiber species showed that the cp genome could not guarantee the accuracy of identification. An untargeted metabolomics strategy combining GC-MS with chemometric methods was proposed to distinguish the Zingiber samples of known variety. A total of 51 volatile compounds extracted from Z. corallinum and Z. montanum were identified, and nine compounds were selected as candidate metabolic markers to reveal the significant difference between Z. corallinum and Z. montanum. The performance of the untargeted metabolomic approach was verified with unknown Zingiber samples. Although the cp genomes could not be used to identify Zingiber species in this study, it will still provide a valuable genomics resource for population studies in the Zingiberaceae family, and the GC-MS based metabolic fingerprint is more promising for species identification and safe application of Z. corallinum and Z. montanum.
format	article
author	Chenxi Wang Yue Zhang Hui Ding Meifang Song Jiaxin Yin Heshui Yu Zheng Li Lifeng Han Zhonglian Zhang
author_facet	Chenxi Wang Yue Zhang Hui Ding Meifang Song Jiaxin Yin Heshui Yu Zheng Li Lifeng Han Zhonglian Zhang
author_sort	Chenxi Wang
title	Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
title_short	Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
title_full	Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
title_fullStr	Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
title_full_unstemmed	Authentication of Zingiber Species Based on Analysis of Metabolite Profiles
title_sort	authentication of zingiber species based on analysis of metabolite profiles
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/63a2206568d24d509d6db5a5413a6a34
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_version_	1718406686182473728

Authentication of Zingiber Species Based on Analysis of Metabolite Profiles

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