Effect of seed coating with biological agents on seed quality of rice

Palupi T, Ilyas S, Machmud M, Widajati E. 2017. Effect of seed coating with biological agents on seed quality of rice. Biodiversitas 18: 727-732. The research that consisted of two activities was performed at the Laboratory. Experiment 1 the aim to obtain biological agents that have high antagonisti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	TANTRI PALUPI, SATRIYAS ILYAS, MUHAMMAD MACHMUD, ENY WIDAJATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	antagonistic bacillus subtilis 5/b growth compatibility inhibition zone pseudomonas diminuta a6 rice seeds coated Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/63a43b7513ea44808e48dfa5ba62610a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:63a43b7513ea44808e48dfa5ba62610a
record_format	dspace
spelling	oai:doaj.org-article:63a43b7513ea44808e48dfa5ba62610a2021-11-16T13:52:30ZEffect of seed coating with biological agents on seed quality of rice1412-033X2085-472210.13057/biodiv/d180245https://doaj.org/article/63a43b7513ea44808e48dfa5ba62610a2017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1704https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Palupi T, Ilyas S, Machmud M, Widajati E. 2017. Effect of seed coating with biological agents on seed quality of rice. Biodiversitas 18: 727-732. The research that consisted of two activities was performed at the Laboratory. Experiment 1 the aim to obtain biological agents that have high antagonistic potential against Xanthomonas oryzae pv. oryzae (Xoo) and two biological agents that are compatible to one another. Experiments 2 to determine the effect of rice (Oryza sativa L.) seeds coated with biological agents to improve seed quality and reduce levels of Xoo infection. In the first experiment, four isolates of bacterial antagonists, i.e: isolate Pseudomonas diminuta A6; isolate P. aeruginosa A54; isolate Bacillus subtilis 11/C, and isolate B. subtilis 5/B, were tested for their antagonism activities against Xoo on PSA plates using the method of growth inhibition zone with filter paper. Furthermore, the four antagonists were tested for their compatibility with each other on PSA plates. In the second experiment used a completely randomized design with a single factor (seed coating) consisting of seven levels, namely: negative control, healthy seed; positive control, the seeds contaminated with Xoo; P. diminuta A6 and B. subtilis 5/B; alginate 3% + 1% peat + P. diminuta A6 and B. subtilis % 5/B; arabic gum 3% + 1% gypsum + P. diminuta A6 and B. subtilis 5/B; CMC 1.5% + 1% talc + P. diminuta A6 and B. subtilis 5/B, and bactericide streptomycin sulfate 20%. Results of Experiment 1 showed that isolates P. diminuta A6 has the highest antagonistic potential against Xoo based on the diameter of inhibition zone on Xoo, followed by isolate B. subtilis 5/B. Isolate P. diminuta A6 and B. subtilis 5/B had a good growth compatibility with no antagonism. Therefore, isolates P. diminuta A6 and B. subtilis 5/B were selected and used as biocontrol agents in further studies. Results of Experiment 2 showed that treatment with P. diminuta A6 + B. subtilis 5/B gave the best of the increase of seed vigor, and the decrease of Xoo infection level in the seeds.TANTRI PALUPISATRIYAS ILYASMUHAMMAD MACHMUDENY WIDAJATIMBI & UNS Soloarticleantagonisticbacillus subtilis 5/bgrowth compatibilityinhibition zonepseudomonas diminuta a6rice seeds coatedBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 727-732 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antagonistic bacillus subtilis 5/b growth compatibility inhibition zone pseudomonas diminuta a6 rice seeds coated Biology (General) QH301-705.5
spellingShingle	antagonistic bacillus subtilis 5/b growth compatibility inhibition zone pseudomonas diminuta a6 rice seeds coated Biology (General) QH301-705.5 TANTRI PALUPI SATRIYAS ILYAS MUHAMMAD MACHMUD ENY WIDAJATI Effect of seed coating with biological agents on seed quality of rice
description	Palupi T, Ilyas S, Machmud M, Widajati E. 2017. Effect of seed coating with biological agents on seed quality of rice. Biodiversitas 18: 727-732. The research that consisted of two activities was performed at the Laboratory. Experiment 1 the aim to obtain biological agents that have high antagonistic potential against Xanthomonas oryzae pv. oryzae (Xoo) and two biological agents that are compatible to one another. Experiments 2 to determine the effect of rice (Oryza sativa L.) seeds coated with biological agents to improve seed quality and reduce levels of Xoo infection. In the first experiment, four isolates of bacterial antagonists, i.e: isolate Pseudomonas diminuta A6; isolate P. aeruginosa A54; isolate Bacillus subtilis 11/C, and isolate B. subtilis 5/B, were tested for their antagonism activities against Xoo on PSA plates using the method of growth inhibition zone with filter paper. Furthermore, the four antagonists were tested for their compatibility with each other on PSA plates. In the second experiment used a completely randomized design with a single factor (seed coating) consisting of seven levels, namely: negative control, healthy seed; positive control, the seeds contaminated with Xoo; P. diminuta A6 and B. subtilis 5/B; alginate 3% + 1% peat + P. diminuta A6 and B. subtilis % 5/B; arabic gum 3% + 1% gypsum + P. diminuta A6 and B. subtilis 5/B; CMC 1.5% + 1% talc + P. diminuta A6 and B. subtilis 5/B, and bactericide streptomycin sulfate 20%. Results of Experiment 1 showed that isolates P. diminuta A6 has the highest antagonistic potential against Xoo based on the diameter of inhibition zone on Xoo, followed by isolate B. subtilis 5/B. Isolate P. diminuta A6 and B. subtilis 5/B had a good growth compatibility with no antagonism. Therefore, isolates P. diminuta A6 and B. subtilis 5/B were selected and used as biocontrol agents in further studies. Results of Experiment 2 showed that treatment with P. diminuta A6 + B. subtilis 5/B gave the best of the increase of seed vigor, and the decrease of Xoo infection level in the seeds.
format	article
author	TANTRI PALUPI SATRIYAS ILYAS MUHAMMAD MACHMUD ENY WIDAJATI
author_facet	TANTRI PALUPI SATRIYAS ILYAS MUHAMMAD MACHMUD ENY WIDAJATI
author_sort	TANTRI PALUPI
title	Effect of seed coating with biological agents on seed quality of rice
title_short	Effect of seed coating with biological agents on seed quality of rice
title_full	Effect of seed coating with biological agents on seed quality of rice
title_fullStr	Effect of seed coating with biological agents on seed quality of rice
title_full_unstemmed	Effect of seed coating with biological agents on seed quality of rice
title_sort	effect of seed coating with biological agents on seed quality of rice
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/63a43b7513ea44808e48dfa5ba62610a
work_keys_str_mv	AT tantripalupi effectofseedcoatingwithbiologicalagentsonseedqualityofrice AT satriyasilyas effectofseedcoatingwithbiologicalagentsonseedqualityofrice AT muhammadmachmud effectofseedcoatingwithbiologicalagentsonseedqualityofrice AT enywidajati effectofseedcoatingwithbiologicalagentsonseedqualityofrice
_version_	1718426483818496000

Effect of seed coating with biological agents on seed quality of rice

Ejemplares similares