Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas

Abstract Despite the dramatic increase in the prevalence of diabetes, techniques for in situ studies of the underlying pancreatic biochemistry are lacking. Such methods would facilitate obtaining mechanistic understanding of diabetes pathophysiology and aid in prognostic and/or diagnostic assessment...

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Autores principales:	Christoffer Nord, Maria Eriksson, Andrea Dicker, Anna Eriksson, Eivind Grong, Erwin Ilegems, Ronald Mårvik, Bård Kulseng, Per-Olof Berggren, András Gorzsás, Ulf Ahlgren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63adc14eb213420d99da8765b024c084
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spelling	oai:doaj.org-article:63adc14eb213420d99da8765b024c0842021-12-02T11:50:57ZBiochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas10.1038/s41598-017-07015-z2045-2322https://doaj.org/article/63adc14eb213420d99da8765b024c0842017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07015-zhttps://doaj.org/toc/2045-2322Abstract Despite the dramatic increase in the prevalence of diabetes, techniques for in situ studies of the underlying pancreatic biochemistry are lacking. Such methods would facilitate obtaining mechanistic understanding of diabetes pathophysiology and aid in prognostic and/or diagnostic assessments. In this report we demonstrate how a multivariate imaging approach (orthogonal projections to latent structures - discriminant analysis) can be applied to generate full vibrational microspectroscopic profiles of pancreatic tissues. These profiles enable extraction of known and previously unrecorded biochemical alterations in models of diabetes, and allow for classification of the investigated tissue with regards to tissue type, strain and stage of disease progression. Most significantly, the approach provided evidence for dramatic alterations of the pancreatic biochemistry at the initial onset of immune-infiltration in the Non Obese Diabetic model for type 1 diabetes. Further, it enabled detection of a previously undocumented accumulation of collagen fibrils in the leptin deficient ob/ob mouse islets. By generating high quality spectral profiles through the tissue capsule of hydrated human pancreata and by in vivo Raman imaging of pancreatic islets transplanted to the anterior chamber of the eye, we provide critical feasibility studies for the translation of this technique to diagnostic assessments of pancreatic biochemistry in vivo.Christoffer NordMaria ErikssonAndrea DickerAnna ErikssonEivind GrongErwin IlegemsRonald MårvikBård KulsengPer-Olof BerggrenAndrás GorzsásUlf AhlgrenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christoffer Nord Maria Eriksson Andrea Dicker Anna Eriksson Eivind Grong Erwin Ilegems Ronald Mårvik Bård Kulseng Per-Olof Berggren András Gorzsás Ulf Ahlgren Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
description	Abstract Despite the dramatic increase in the prevalence of diabetes, techniques for in situ studies of the underlying pancreatic biochemistry are lacking. Such methods would facilitate obtaining mechanistic understanding of diabetes pathophysiology and aid in prognostic and/or diagnostic assessments. In this report we demonstrate how a multivariate imaging approach (orthogonal projections to latent structures - discriminant analysis) can be applied to generate full vibrational microspectroscopic profiles of pancreatic tissues. These profiles enable extraction of known and previously unrecorded biochemical alterations in models of diabetes, and allow for classification of the investigated tissue with regards to tissue type, strain and stage of disease progression. Most significantly, the approach provided evidence for dramatic alterations of the pancreatic biochemistry at the initial onset of immune-infiltration in the Non Obese Diabetic model for type 1 diabetes. Further, it enabled detection of a previously undocumented accumulation of collagen fibrils in the leptin deficient ob/ob mouse islets. By generating high quality spectral profiles through the tissue capsule of hydrated human pancreata and by in vivo Raman imaging of pancreatic islets transplanted to the anterior chamber of the eye, we provide critical feasibility studies for the translation of this technique to diagnostic assessments of pancreatic biochemistry in vivo.
format	article
author	Christoffer Nord Maria Eriksson Andrea Dicker Anna Eriksson Eivind Grong Erwin Ilegems Ronald Mårvik Bård Kulseng Per-Olof Berggren András Gorzsás Ulf Ahlgren
author_facet	Christoffer Nord Maria Eriksson Andrea Dicker Anna Eriksson Eivind Grong Erwin Ilegems Ronald Mårvik Bård Kulseng Per-Olof Berggren András Gorzsás Ulf Ahlgren
author_sort	Christoffer Nord
title	Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
title_short	Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
title_full	Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
title_fullStr	Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
title_full_unstemmed	Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
title_sort	biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63adc14eb213420d99da8765b024c084
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Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas

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