Flagellin-based electrochemical sensing layer for arsenic detection in water

Abstract Regular monitoring of arsenic concentrations in water sources is essential due to the severe health effects. Our goal was to develop a rapidly responding, sensitive and stable sensing layer for the detection of arsenic. We have designed flagellin-based arsenic binding proteins capable of fo...

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Autores principales:	Hajnalka Jankovics, Patrik Szekér, Éva Tóth, Balázs Kakasi, Zoltán Lábadi, András Saftics, Benjamin Kalas, Miklós Fried, Péter Petrik, Ferenc Vonderviszt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63aeb5f258454088ad40800bff5bebe5
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spelling	oai:doaj.org-article:63aeb5f258454088ad40800bff5bebe52021-12-02T14:27:02ZFlagellin-based electrochemical sensing layer for arsenic detection in water10.1038/s41598-021-83053-y2045-2322https://doaj.org/article/63aeb5f258454088ad40800bff5bebe52021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83053-yhttps://doaj.org/toc/2045-2322Abstract Regular monitoring of arsenic concentrations in water sources is essential due to the severe health effects. Our goal was to develop a rapidly responding, sensitive and stable sensing layer for the detection of arsenic. We have designed flagellin-based arsenic binding proteins capable of forming stable filament structures with high surface binding site densities. The D3 domain of Salmonella typhimurium flagellin was replaced with an arsenic-binding peptide motif of different bacterial ArsR transcriptional repressor factors. We have shown that the fusion proteins developed retain their polymerization ability and have thermal stability similar to that of wild-type filament. The strong arsenic binding capacity of the monomeric proteins was confirmed by isothermal titration calorimetry (ITC), and dissociation constants (Kd) of a few hundred nM were obtained for all three variants. As-binding fibers were immobilized on the surface of a gold electrode and used as a working electrode in cyclic voltammetry (CV) experiments to detect inorganic arsenic near the maximum allowable concentration (MAC) level. Based on these results, it can be concluded that the stable arsenic-binding flagellin variant can be used as a rapidly responding, sensitive, but simple sensing layer in a field device for the MAC-level detection of arsenic in natural waters.Hajnalka JankovicsPatrik SzekérÉva TóthBalázs KakasiZoltán LábadiAndrás SafticsBenjamin KalasMiklós FriedPéter PetrikFerenc VondervisztNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hajnalka Jankovics Patrik Szekér Éva Tóth Balázs Kakasi Zoltán Lábadi András Saftics Benjamin Kalas Miklós Fried Péter Petrik Ferenc Vonderviszt Flagellin-based electrochemical sensing layer for arsenic detection in water
description	Abstract Regular monitoring of arsenic concentrations in water sources is essential due to the severe health effects. Our goal was to develop a rapidly responding, sensitive and stable sensing layer for the detection of arsenic. We have designed flagellin-based arsenic binding proteins capable of forming stable filament structures with high surface binding site densities. The D3 domain of Salmonella typhimurium flagellin was replaced with an arsenic-binding peptide motif of different bacterial ArsR transcriptional repressor factors. We have shown that the fusion proteins developed retain their polymerization ability and have thermal stability similar to that of wild-type filament. The strong arsenic binding capacity of the monomeric proteins was confirmed by isothermal titration calorimetry (ITC), and dissociation constants (Kd) of a few hundred nM were obtained for all three variants. As-binding fibers were immobilized on the surface of a gold electrode and used as a working electrode in cyclic voltammetry (CV) experiments to detect inorganic arsenic near the maximum allowable concentration (MAC) level. Based on these results, it can be concluded that the stable arsenic-binding flagellin variant can be used as a rapidly responding, sensitive, but simple sensing layer in a field device for the MAC-level detection of arsenic in natural waters.
format	article
author	Hajnalka Jankovics Patrik Szekér Éva Tóth Balázs Kakasi Zoltán Lábadi András Saftics Benjamin Kalas Miklós Fried Péter Petrik Ferenc Vonderviszt
author_facet	Hajnalka Jankovics Patrik Szekér Éva Tóth Balázs Kakasi Zoltán Lábadi András Saftics Benjamin Kalas Miklós Fried Péter Petrik Ferenc Vonderviszt
author_sort	Hajnalka Jankovics
title	Flagellin-based electrochemical sensing layer for arsenic detection in water
title_short	Flagellin-based electrochemical sensing layer for arsenic detection in water
title_full	Flagellin-based electrochemical sensing layer for arsenic detection in water
title_fullStr	Flagellin-based electrochemical sensing layer for arsenic detection in water
title_full_unstemmed	Flagellin-based electrochemical sensing layer for arsenic detection in water
title_sort	flagellin-based electrochemical sensing layer for arsenic detection in water
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63aeb5f258454088ad40800bff5bebe5
work_keys_str_mv	AT hajnalkajankovics flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT patrikszeker flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT evatoth flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT balazskakasi flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT zoltanlabadi flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT andrassaftics flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT benjaminkalas flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT miklosfried flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT peterpetrik flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater AT ferencvonderviszt flagellinbasedelectrochemicalsensinglayerforarsenicdetectioninwater
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Flagellin-based electrochemical sensing layer for arsenic detection in water

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