Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells

Hanouf Almukhlafi, Daoud Ali, Bader Almutairi, Khadijah N Yaseen, Nouf Alyami, Rafa Almeer, Saad Alkahtani, Saud Alarifi Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment of Zoology, College of Science, King Saud University, B...

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Autores principales:	Almukhlafi H, Ali D, Almutairi B, Yaseen KN, Alyami N, Almeer R, Alkahtani S, Alarifi S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	la2o3 nps apoptosis oxidative stress chang &huh-7 cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/63b63f3847324191b3d2e23e37a558a6
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spelling	oai:doaj.org-article:63b63f3847324191b3d2e23e37a558a62021-12-02T18:22:12ZRole of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells1178-2013https://doaj.org/article/63b63f3847324191b3d2e23e37a558a62021-05-01T00:00:00Zhttps://www.dovepress.com/role-of-oxidative-stress-in-la2o3-nanoparticle-induced-cytotoxicity-an-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Hanouf Almukhlafi, Daoud Ali, Bader Almutairi, Khadijah N Yaseen, Nouf Alyami, Rafa Almeer, Saad Alkahtani, Saud Alarifi Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment of Zoology, College of Science, King Saud University, BOX 2455, Riyadh, 11451, Saudi ArabiaTel +966 11 4679816Fax +966 11 4678514Email salarifi@ksu.edu.saIntroduction: Nanoparticles are extensively applied in pharmaceutical, agriculture, food processing industries, and in many other fields. In the current experiment, we have determined the mechanism of toxicity of lanthanum oxide nanoparticles (La2O3 NPs) on human liver cell lines.Methods: Before the investigation, we have characterized the size and shape of La2O3 NPs using dynamic light scattering (DLS) and transmission electron microscope (TEM). The mean size of the La2O3 NPs was found 32 ± 1.6 nm with a sheet-like shape. The cytotoxicity effect of La2O3 NPs for 24 h on CHANG and HuH-7 cells was determined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays.Results: The cytotoxicity was observed in a concentration-dependent manner in both cells but NPs were more toxic to HuH-7 than CHANG cells. Generation of reactive oxygen species (ROS) was determined using fluorescent dye 2′,7′-dichlorofluorescin diacetate (DCFDA) and high green fluorescence was observed in HuH-7 cells than CHANG cells. Oxidative stress biomarker such as glutathione (GSH) was decreased and antioxidant enzyme superoxide dismutase (SOD) was increased but SOD level was decreased in HuH-7 cells than CHANG cells. Apoptotic cells were determined by using fluorescence-activated cell sorting (FACS) analysis. Maximum percentage of the apoptotic cell was observed at 300 μg/mL in HuH-7 cells. DNA double-stranded breakage was observed by comet assay and maximum DNA damage was found in CHANG cells than HuH-7 cells at 300 μg/mL La2O3 NPs for 24 h.Conclusion: Thus, this study demonstrated that La2O3 NPs were toxic to human liver cells and induced more toxicity in HuH-7 cells than CHANG cells.Keywords: La2O3 NPs, apoptosis, oxidative stress, CHANG and HuH-7 cellsAlmukhlafi HAli DAlmutairi BYaseen KNAlyami NAlmeer RAlkahtani SAlarifi SDove Medical Pressarticlela2o3 npsapoptosisoxidative stresschang &huh-7 cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3487-3496 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	la2o3 nps apoptosis oxidative stress chang &huh-7 cells Medicine (General) R5-920
spellingShingle	la2o3 nps apoptosis oxidative stress chang &huh-7 cells Medicine (General) R5-920 Almukhlafi H Ali D Almutairi B Yaseen KN Alyami N Almeer R Alkahtani S Alarifi S Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
description	Hanouf Almukhlafi, Daoud Ali, Bader Almutairi, Khadijah N Yaseen, Nouf Alyami, Rafa Almeer, Saad Alkahtani, Saud Alarifi Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaCorrespondence: Saud AlarifiDepartment of Zoology, College of Science, King Saud University, BOX 2455, Riyadh, 11451, Saudi ArabiaTel +966 11 4679816Fax +966 11 4678514Email salarifi@ksu.edu.saIntroduction: Nanoparticles are extensively applied in pharmaceutical, agriculture, food processing industries, and in many other fields. In the current experiment, we have determined the mechanism of toxicity of lanthanum oxide nanoparticles (La2O3 NPs) on human liver cell lines.Methods: Before the investigation, we have characterized the size and shape of La2O3 NPs using dynamic light scattering (DLS) and transmission electron microscope (TEM). The mean size of the La2O3 NPs was found 32 ± 1.6 nm with a sheet-like shape. The cytotoxicity effect of La2O3 NPs for 24 h on CHANG and HuH-7 cells was determined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays.Results: The cytotoxicity was observed in a concentration-dependent manner in both cells but NPs were more toxic to HuH-7 than CHANG cells. Generation of reactive oxygen species (ROS) was determined using fluorescent dye 2′,7′-dichlorofluorescin diacetate (DCFDA) and high green fluorescence was observed in HuH-7 cells than CHANG cells. Oxidative stress biomarker such as glutathione (GSH) was decreased and antioxidant enzyme superoxide dismutase (SOD) was increased but SOD level was decreased in HuH-7 cells than CHANG cells. Apoptotic cells were determined by using fluorescence-activated cell sorting (FACS) analysis. Maximum percentage of the apoptotic cell was observed at 300 μg/mL in HuH-7 cells. DNA double-stranded breakage was observed by comet assay and maximum DNA damage was found in CHANG cells than HuH-7 cells at 300 μg/mL La2O3 NPs for 24 h.Conclusion: Thus, this study demonstrated that La2O3 NPs were toxic to human liver cells and induced more toxicity in HuH-7 cells than CHANG cells.Keywords: La2O3 NPs, apoptosis, oxidative stress, CHANG and HuH-7 cells
format	article
author	Almukhlafi H Ali D Almutairi B Yaseen KN Alyami N Almeer R Alkahtani S Alarifi S
author_facet	Almukhlafi H Ali D Almutairi B Yaseen KN Alyami N Almeer R Alkahtani S Alarifi S
author_sort	Almukhlafi H
title	Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
title_short	Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
title_full	Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
title_fullStr	Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
title_full_unstemmed	Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells
title_sort	role of oxidative stress in la2o3 nanoparticle-induced cytotoxicity and apoptosis in chang and huh-7 cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/63b63f3847324191b3d2e23e37a558a6
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Role of Oxidative Stress in La2O3 Nanoparticle-Induced Cytotoxicity and Apoptosis in CHANG and HuH-7 Cells

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