Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µ...

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Autores principales:	Kateřina Skopalová, Katarzyna Anna Radaszkiewicz, Markéta Kadlečková, Jiří Pacherník, Antonín Minařík, Zdenka Capáková, Věra Kašpárková, Aleš Mráček, Eliška Daďová, Petr Humpolíček
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biomimetic surfaces human-induced pluripotent stem cells fluorescence signal cardiomyogenesis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/63c113fdf8c44fd3898603fa17024988
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spelling	oai:doaj.org-article:63c113fdf8c44fd3898603fa170249882021-11-11T17:21:41ZHierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell10.3390/ijms2221119431422-00671661-6596https://doaj.org/article/63c113fdf8c44fd3898603fa170249882021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11943https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm<sup>2</sup>; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm<sup>2</sup>) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca<sup>2+</sup> flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.Kateřina SkopalováKatarzyna Anna RadaszkiewiczMarkéta KadlečkováJiří PacherníkAntonín MinaříkZdenka CapákováVěra KašpárkováAleš MráčekEliška DaďováPetr HumpolíčekMDPI AGarticlebiomimeticsurfaceshuman-induced pluripotent stem cellsfluorescence signalcardiomyogenesisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11943, p 11943 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biomimetic surfaces human-induced pluripotent stem cells fluorescence signal cardiomyogenesis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	biomimetic surfaces human-induced pluripotent stem cells fluorescence signal cardiomyogenesis Biology (General) QH301-705.5 Chemistry QD1-999 Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
description	An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm<sup>2</sup>; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm<sup>2</sup>; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm<sup>2</sup>) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca<sup>2+</sup> flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.
format	article
author	Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček
author_facet	Kateřina Skopalová Katarzyna Anna Radaszkiewicz Markéta Kadlečková Jiří Pacherník Antonín Minařík Zdenka Capáková Věra Kašpárková Aleš Mráček Eliška Daďová Petr Humpolíček
author_sort	Kateřina Skopalová
title	Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
title_short	Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
title_full	Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
title_fullStr	Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
title_full_unstemmed	Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
title_sort	hierarchically structured polystyrene-based surfaces amplifying fluorescence signals: cytocompatibility with human induced pluripotent stem cell
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/63c113fdf8c44fd3898603fa17024988
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Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

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