THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM

Abstract. The detection of cystinase activity is one of most important tests in the laboratory diagnosis of diphtheria, giving the opportunity to differentiate potential toxigenic species from other coryneform bacteria. The original recipe of Pizu media for detection of cystinase activity, proposed...

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Autor principal:	S. A. Gabrielyan
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	corynebacterium the cistinaze activity the modified nutrient medium Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/63c2ab7902ce42638948747f41e8db30
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spelling	oai:doaj.org-article:63c2ab7902ce42638948747f41e8db302021-11-22T07:09:46ZTHE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM2220-76192313-739810.15789/2220-7619-2013-4-347-350https://doaj.org/article/63c2ab7902ce42638948747f41e8db302014-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/144https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. The detection of cystinase activity is one of most important tests in the laboratory diagnosis of diphtheria, giving the opportunity to differentiate potential toxigenic species from other coryneform bacteria. The original recipe of Pizu media for detection of cystinase activity, proposed in 1939–1940, was modified several times (1982, 1989) for different reasons. In the last modification the Pizu media was prepared by using the AGV media as a nutritional base. We suggest a modification of Pizu media using Mueller-Hinton agar as nutritional basis. The Mueller-Hinton agar has a number of advantages: higher nutritional value, standardization, transparency and availability. A positive result is defined within 2–4 hours after inoculation of enough quantity of material (pure culture or C. diphtheriaе in association with other microorganisms) as a brown halo surrounding black colonies. The brown halo does not appear in the upper part of the media (0,5–1 cm). The modified media was checked with 21 strains of C. diphtheriaе (tox+), nine strains of coryneform bacteria, control strains NCTC 10648, NCTC 10356, NCTC 3984. The modified Pizu media is registered in the RA mental property agency as an invention (no. 1877 A2, 15.12.2006).S. A. GabrielyanSankt-Peterburg : NIIÈM imeni Pasteraarticlecorynebacteriumthe cistinaze activitythe modified nutrient mediumInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 3, Iss 4, Pp 347-350 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	corynebacterium the cistinaze activity the modified nutrient medium Infectious and parasitic diseases RC109-216
spellingShingle	corynebacterium the cistinaze activity the modified nutrient medium Infectious and parasitic diseases RC109-216 S. A. Gabrielyan THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
description	Abstract. The detection of cystinase activity is one of most important tests in the laboratory diagnosis of diphtheria, giving the opportunity to differentiate potential toxigenic species from other coryneform bacteria. The original recipe of Pizu media for detection of cystinase activity, proposed in 1939–1940, was modified several times (1982, 1989) for different reasons. In the last modification the Pizu media was prepared by using the AGV media as a nutritional base. We suggest a modification of Pizu media using Mueller-Hinton agar as nutritional basis. The Mueller-Hinton agar has a number of advantages: higher nutritional value, standardization, transparency and availability. A positive result is defined within 2–4 hours after inoculation of enough quantity of material (pure culture or C. diphtheriaе in association with other microorganisms) as a brown halo surrounding black colonies. The brown halo does not appear in the upper part of the media (0,5–1 cm). The modified media was checked with 21 strains of C. diphtheriaе (tox+), nine strains of coryneform bacteria, control strains NCTC 10648, NCTC 10356, NCTC 3984. The modified Pizu media is registered in the RA mental property agency as an invention (no. 1877 A2, 15.12.2006).
format	article
author	S. A. Gabrielyan
author_facet	S. A. Gabrielyan
author_sort	S. A. Gabrielyan
title	THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
title_short	THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
title_full	THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
title_fullStr	THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
title_full_unstemmed	THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM
title_sort	accelerated method for identification of pathogenic corynebacterium
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/63c2ab7902ce42638948747f41e8db30
work_keys_str_mv	AT sagabrielyan theacceleratedmethodforidentificationofpathogeniccorynebacterium AT sagabrielyan acceleratedmethodforidentificationofpathogeniccorynebacterium
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THE ACCELERATED METHOD FOR IDENTIFICATION OF PATHOGENIC CORYNEBACTERIUM

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