Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis

Abstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples followi...

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Autores principales:	Yongchun Shen, Xiaorong Zhang, Wenyi Lin, Chun Wan, Qiyuan Li, Yong Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63c417934829441eb4e5ebde576ab3af
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spelling	oai:doaj.org-article:63c417934829441eb4e5ebde576ab3af2021-12-02T16:06:58ZLiquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis10.1038/s41598-017-07564-32045-2322https://doaj.org/article/63c417934829441eb4e5ebde576ab3af2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07564-3https://doaj.org/toc/2045-2322Abstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou’s or Special staining with Grocott’s methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou’s staining, LCT-prepared slides allowed a higher positive rate of Aspergillus identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, P < 0.05) and sputum samples (29.05% vs. 8.55%, P < 0.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of Aspergillus identification for bronchial brushing samples (83.33% vs. 57.41%, P < 0.05) and sputum samples (43.59% vs. 19.66%, P < 0.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying Aspergillus in respiratory samples from patients with pulmonary aspergillosis.Yongchun ShenXiaorong ZhangWenyi LinChun WanQiyuan LiYong JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
description	Abstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou’s or Special staining with Grocott’s methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou’s staining, LCT-prepared slides allowed a higher positive rate of Aspergillus identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, P < 0.05) and sputum samples (29.05% vs. 8.55%, P < 0.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of Aspergillus identification for bronchial brushing samples (83.33% vs. 57.41%, P < 0.05) and sputum samples (43.59% vs. 19.66%, P < 0.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying Aspergillus in respiratory samples from patients with pulmonary aspergillosis.
format	article
author	Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang
author_facet	Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang
author_sort	Yongchun Shen
title	Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
title_short	Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
title_full	Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
title_fullStr	Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
title_full_unstemmed	Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
title_sort	liquid-based cytopathology test as a novel method to identify aspergillus in patients with pulmonary aspergillosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63c417934829441eb4e5ebde576ab3af
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Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis

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