Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice

Abstract Adolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we an...

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Autores principales:	Fausto Chiazza, Heather Bondi, Irene Masante, Federico Ugazio, Valeria Bortolotto, Pier Luigi Canonico, Mariagrazia Grilli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63c624f2fbc74fee8c3b0f4905b7c571
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spelling	oai:doaj.org-article:63c624f2fbc74fee8c3b0f4905b7c5712021-11-08T10:48:16ZShort high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice10.1038/s41598-021-01059-y2045-2322https://doaj.org/article/63c624f2fbc74fee8c3b0f4905b7c5712021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01059-yhttps://doaj.org/toc/2045-2322Abstract Adolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we analyzed the effects of a short period of hypercaloric diet on newborn hippocampal doublecortin+ (DCX) immature neurons in adolescent mice. Male mice received high fat diet (HFD) or control low fat diet (LFD) from the 5th week of age for 1 or 2 weeks, or 1 week HFD followed by 1 week LFD. After diet supply, mice were either perfused for immunohistochemical (IHC) analysis or their hippocampi were dissected for biochemical assays. Detailed morphometric analysis was performed in DCX+ cells that displayed features of immature neurons. We report that 1 week-HFD was sufficient to dramatically reduce dendritic tree complexity of DCX+ cells. This effect occurred specifically in dorsal and not ventral hippocampus and correlated with reduced BDNF expression levels in dorsal hippocampus. Both structural and biochemical changes were reversed by a return to LFD. Altogether these studies increase our current knowledge on potential consequences of hypercaloric diet on brain and in particular on dorsal hippocampal neuroplasticity.Fausto ChiazzaHeather BondiIrene MasanteFederico UgazioValeria BortolottoPier Luigi CanonicoMariagrazia GrilliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fausto Chiazza Heather Bondi Irene Masante Federico Ugazio Valeria Bortolotto Pier Luigi Canonico Mariagrazia Grilli Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
description	Abstract Adolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we analyzed the effects of a short period of hypercaloric diet on newborn hippocampal doublecortin+ (DCX) immature neurons in adolescent mice. Male mice received high fat diet (HFD) or control low fat diet (LFD) from the 5th week of age for 1 or 2 weeks, or 1 week HFD followed by 1 week LFD. After diet supply, mice were either perfused for immunohistochemical (IHC) analysis or their hippocampi were dissected for biochemical assays. Detailed morphometric analysis was performed in DCX+ cells that displayed features of immature neurons. We report that 1 week-HFD was sufficient to dramatically reduce dendritic tree complexity of DCX+ cells. This effect occurred specifically in dorsal and not ventral hippocampus and correlated with reduced BDNF expression levels in dorsal hippocampus. Both structural and biochemical changes were reversed by a return to LFD. Altogether these studies increase our current knowledge on potential consequences of hypercaloric diet on brain and in particular on dorsal hippocampal neuroplasticity.
format	article
author	Fausto Chiazza Heather Bondi Irene Masante Federico Ugazio Valeria Bortolotto Pier Luigi Canonico Mariagrazia Grilli
author_facet	Fausto Chiazza Heather Bondi Irene Masante Federico Ugazio Valeria Bortolotto Pier Luigi Canonico Mariagrazia Grilli
author_sort	Fausto Chiazza
title	Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
title_short	Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
title_full	Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
title_fullStr	Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
title_full_unstemmed	Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice
title_sort	short high fat diet triggers reversible and region specific effects in dcx+ hippocampal immature neurons of adolescent male mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63c624f2fbc74fee8c3b0f4905b7c571
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Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice

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