Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause

Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active...

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Autores principales:	Ajdžanović Vladimir, Medigović Ivana, Živanović Jasmina, Šošić-Jurjević Branka, Trifunović Svetlana, Tanić Nasta, Milošević Verica
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	andropause gh cells immuno-fluorescence immuno-histochemistry soy isoflavones Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/63cd7c5028a443328e126db4671d88b2
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spelling	oai:doaj.org-article:63cd7c5028a443328e126db4671d88b22021-11-17T21:27:52ZImmuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause1820-744810.2478/acve-2014-0010https://doaj.org/article/63cd7c5028a443328e126db4671d88b22014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0010https://doaj.org/toc/1820-7448Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p<0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immunohistomorphometric and -fluorescent characteristics of growth hormone secreting cells.Ajdžanović VladimirMedigović IvanaŽivanović JasminaŠošić-Jurjević BrankaTrifunović SvetlanaTanić NastaMilošević VericaSciendoarticleandropausegh cellsimmuno-fluorescenceimmuno-histochemistrysoy isoflavonesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 93-104 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	andropause gh cells immuno-fluorescence immuno-histochemistry soy isoflavones Veterinary medicine SF600-1100
spellingShingle	andropause gh cells immuno-fluorescence immuno-histochemistry soy isoflavones Veterinary medicine SF600-1100 Ajdžanović Vladimir Medigović Ivana Živanović Jasmina Šošić-Jurjević Branka Trifunović Svetlana Tanić Nasta Milošević Verica Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
description	Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p<0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immunohistomorphometric and -fluorescent characteristics of growth hormone secreting cells.
format	article
author	Ajdžanović Vladimir Medigović Ivana Živanović Jasmina Šošić-Jurjević Branka Trifunović Svetlana Tanić Nasta Milošević Verica
author_facet	Ajdžanović Vladimir Medigović Ivana Živanović Jasmina Šošić-Jurjević Branka Trifunović Svetlana Tanić Nasta Milošević Verica
author_sort	Ajdžanović Vladimir
title	Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
title_short	Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
title_full	Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
title_fullStr	Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
title_full_unstemmed	Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
title_sort	immuno-histomorphometric and -fluorescent characteristics of gh cells after treatment with genistein or daidzein in an animal model of andropause
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/63cd7c5028a443328e126db4671d88b2
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Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause

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