An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment

Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play crucial roles in ammonia nitrogen degradation in freshwater lake sediment. However, studies on ammonia-oxidizing microorganisms (AOM) in vertical sediment are still limited, especially with respect to the quantitative analysis...

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Autores principales:	Tong-tong Liu, Hong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Vertical sediment Ammonia-oxidizing archaea Ammonia-oxidizing bacteria DNA and RNA levels Abundance and community structure Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/63d8e447a0f44ce1b51d52eee9b6e00d
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spelling	oai:doaj.org-article:63d8e447a0f44ce1b51d52eee9b6e00d2021-12-01T04:36:40ZAn RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment1470-160X10.1016/j.ecolind.2020.107143https://doaj.org/article/63d8e447a0f44ce1b51d52eee9b6e00d2021-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X20310827https://doaj.org/toc/1470-160XAmmonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play crucial roles in ammonia nitrogen degradation in freshwater lake sediment. However, studies on ammonia-oxidizing microorganisms (AOM) in vertical sediment are still limited, especially with respect to the quantitative analysis of the vertical distribution of active AOM at the RNA level. Herein, by researching the AOM in a 75 cm vertical sediment from Lake Taihu, we found that although the total abundance of AOB throughout the whole vertical sediment surpassed that of AOA, active AOA had a closer correlation with the potential nitrification rate than active AOB. The active transcription abundance of AOM generally decreased with increasing depth, especially for AOA, while that of AOB decreased slightly. Compared to active transcription, the total abundance of AOM varied little. The community structures of AOA and AOB changed significantly with increasing depth, and Group I.1a and Nitrosomonas were the predominant clusters, respectively. Ultimately, our study demonstrated that research methods based on the RNA level can accurately reflect the vertical variation in AOM.Tong-tong LiuHong YangElsevierarticleVertical sedimentAmmonia-oxidizing archaeaAmmonia-oxidizing bacteriaDNA and RNA levelsAbundance and community structureEcologyQH540-549.5ENEcological Indicators, Vol 121, Iss , Pp 107143- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vertical sediment Ammonia-oxidizing archaea Ammonia-oxidizing bacteria DNA and RNA levels Abundance and community structure Ecology QH540-549.5
spellingShingle	Vertical sediment Ammonia-oxidizing archaea Ammonia-oxidizing bacteria DNA and RNA levels Abundance and community structure Ecology QH540-549.5 Tong-tong Liu Hong Yang An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
description	Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play crucial roles in ammonia nitrogen degradation in freshwater lake sediment. However, studies on ammonia-oxidizing microorganisms (AOM) in vertical sediment are still limited, especially with respect to the quantitative analysis of the vertical distribution of active AOM at the RNA level. Herein, by researching the AOM in a 75 cm vertical sediment from Lake Taihu, we found that although the total abundance of AOB throughout the whole vertical sediment surpassed that of AOA, active AOA had a closer correlation with the potential nitrification rate than active AOB. The active transcription abundance of AOM generally decreased with increasing depth, especially for AOA, while that of AOB decreased slightly. Compared to active transcription, the total abundance of AOM varied little. The community structures of AOA and AOB changed significantly with increasing depth, and Group I.1a and Nitrosomonas were the predominant clusters, respectively. Ultimately, our study demonstrated that research methods based on the RNA level can accurately reflect the vertical variation in AOM.
format	article
author	Tong-tong Liu Hong Yang
author_facet	Tong-tong Liu Hong Yang
author_sort	Tong-tong Liu
title	An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
title_short	An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
title_full	An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
title_fullStr	An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
title_full_unstemmed	An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
title_sort	rna-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/63d8e447a0f44ce1b51d52eee9b6e00d
work_keys_str_mv	AT tongtongliu anrnabasedstudyofthedistributionofammoniaoxidizingmicroorganismsinverticalsediment AT hongyang anrnabasedstudyofthedistributionofammoniaoxidizingmicroorganismsinverticalsediment AT tongtongliu rnabasedstudyofthedistributionofammoniaoxidizingmicroorganismsinverticalsediment AT hongyang rnabasedstudyofthedistributionofammoniaoxidizingmicroorganismsinverticalsediment
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An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment

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