Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy
Abstract Ovarian cancer is highly metastatic, with a high frequency of relapse, and is the most fatal gynecologic malignancy in women worldwide. It is important to elevate the drug susceptibility and cytotoxicity of ovarian cancer cells, thereby eliminating resident cancer cells for more effective t...
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Nature Portfolio
2021
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oai:doaj.org-article:63d97e87db234af79d3c1e4526c6bbd32021-12-02T14:37:38ZEnhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy10.1038/s41598-021-87271-22045-2322https://doaj.org/article/63d97e87db234af79d3c1e4526c6bbd32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87271-2https://doaj.org/toc/2045-2322Abstract Ovarian cancer is highly metastatic, with a high frequency of relapse, and is the most fatal gynecologic malignancy in women worldwide. It is important to elevate the drug susceptibility and cytotoxicity of ovarian cancer cells, thereby eliminating resident cancer cells for more effective therapeutic efficacy. Here, we developed a bispecific antibody (BsAb; mPEG × HER2) that can easily provide HER2+ tumor tropism to mPEGylated liposomal doxorubicin (PLD) and further increase the drug accumulation in cancer cells via receptor-mediated endocytosis, and improve the cytotoxicity and therapeutic efficacy of HER2+ ovarian tumors. The mPEG × HER2 can simultaneously bind to mPEG molecules on the surface of PLD and HER2 antigen on the surface of ovarian cancer cells. Simply mixing the mPEG × HER2 with PLD was able to confer HER2 specificity of PLD to HER2+ ovarian cancer cells and efficiently trigger endocytosis and enhance cytotoxicity by 5.4-fold as compared to non-targeted PLD. mPEG × HER2-modified PLD was able to significantly increase the targeting and accumulation of HER2+ ovarian tumor by 220% as compared with non-targeted PLD. It could also significantly improve the anti-tumor activity of PLD (P < 0.05) with minimal obvious toxicity in a tumor-bearing mouse model. We believe that the mPEG × HER2 can significantly improve the therapeutic efficacy, potentially reduce the relapse freqency and thereby achieve good prognosis in ovarian cancer patients.Wen-Wei LinYi-An ChengChia-Ching LiKai-Wen HoHuei-Jen ChenI.-J.u ChenBo-Cheng HuangHui-Ju LiuYun-Chi LuChiu-Min ChengMing-Yii HuangHung-Wen LaiTian-Lu ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Wen-Wei Lin Yi-An Cheng Chia-Ching Li Kai-Wen Ho Huei-Jen Chen I.-J.u Chen Bo-Cheng Huang Hui-Ju Liu Yun-Chi Lu Chiu-Min Cheng Ming-Yii Huang Hung-Wen Lai Tian-Lu Cheng Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| description |
Abstract Ovarian cancer is highly metastatic, with a high frequency of relapse, and is the most fatal gynecologic malignancy in women worldwide. It is important to elevate the drug susceptibility and cytotoxicity of ovarian cancer cells, thereby eliminating resident cancer cells for more effective therapeutic efficacy. Here, we developed a bispecific antibody (BsAb; mPEG × HER2) that can easily provide HER2+ tumor tropism to mPEGylated liposomal doxorubicin (PLD) and further increase the drug accumulation in cancer cells via receptor-mediated endocytosis, and improve the cytotoxicity and therapeutic efficacy of HER2+ ovarian tumors. The mPEG × HER2 can simultaneously bind to mPEG molecules on the surface of PLD and HER2 antigen on the surface of ovarian cancer cells. Simply mixing the mPEG × HER2 with PLD was able to confer HER2 specificity of PLD to HER2+ ovarian cancer cells and efficiently trigger endocytosis and enhance cytotoxicity by 5.4-fold as compared to non-targeted PLD. mPEG × HER2-modified PLD was able to significantly increase the targeting and accumulation of HER2+ ovarian tumor by 220% as compared with non-targeted PLD. It could also significantly improve the anti-tumor activity of PLD (P < 0.05) with minimal obvious toxicity in a tumor-bearing mouse model. We believe that the mPEG × HER2 can significantly improve the therapeutic efficacy, potentially reduce the relapse freqency and thereby achieve good prognosis in ovarian cancer patients. |
| format |
article |
| author |
Wen-Wei Lin Yi-An Cheng Chia-Ching Li Kai-Wen Ho Huei-Jen Chen I.-J.u Chen Bo-Cheng Huang Hui-Ju Liu Yun-Chi Lu Chiu-Min Cheng Ming-Yii Huang Hung-Wen Lai Tian-Lu Cheng |
| author_facet |
Wen-Wei Lin Yi-An Cheng Chia-Ching Li Kai-Wen Ho Huei-Jen Chen I.-J.u Chen Bo-Cheng Huang Hui-Ju Liu Yun-Chi Lu Chiu-Min Cheng Ming-Yii Huang Hung-Wen Lai Tian-Lu Cheng |
| author_sort |
Wen-Wei Lin |
| title |
Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| title_short |
Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| title_full |
Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| title_fullStr |
Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| title_full_unstemmed |
Enhancement of tumor tropism of mPEGylated nanoparticles by anti-mPEG bispecific antibody for ovarian cancer therapy |
| title_sort |
enhancement of tumor tropism of mpegylated nanoparticles by anti-mpeg bispecific antibody for ovarian cancer therapy |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/63d97e87db234af79d3c1e4526c6bbd3 |
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