PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos

Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) represent the most common pollutants in the marine sediments. Previous investigations demonstrated short-term sublethal effects of sediments polluted with both contaminants on the sea urchin <i>Paracentrotus lividus&l...

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Autores principales:	Luisa Albarano, Valerio Zupo, Marco Guida, Giovanni Libralato, Davide Caramiello, Nadia Ruocco, Maria Costantini
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aromatic hydrocarbons polychlorinated biphenyls sea urchin Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/63db73b3b59e496590ed902772bf5fcd
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spelling	oai:doaj.org-article:63db73b3b59e496590ed902772bf5fcd2021-11-25T17:57:16ZPAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos10.3390/ijms2222124981422-00671661-6596https://doaj.org/article/63db73b3b59e496590ed902772bf5fcd2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12498https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) represent the most common pollutants in the marine sediments. Previous investigations demonstrated short-term sublethal effects of sediments polluted with both contaminants on the sea urchin <i>Paracentrotus lividus</i> after 2 months of exposure in mesocosms. In particular, morphological malformations observed in <i>P. lividus</i> embryos deriving from adults exposed to PAHs and PCBs were explained at molecular levels by <i>de novo</i> transcriptome assembly and real-time qPCR, leading to the identification of several differentially expressed genes involved in key physiological processes. Here, we extensively explored the genes involved in the response of the sea urchin <i>P. lividus</i> to PAHs and PCBs. Firstly, 25 new genes were identified and interactomic analysis revealed that they were functionally connected among them and to several genes previously defined as molecular targets of response to the two pollutants under analysis. The expression levels of these 25 genes were followed by Real Time qPCR, showing that almost all genes analyzed were affected by PAHs and PCBs. These findings represent an important further step in defining the impacts of slight concentrations of such contaminants on sea urchins and, more in general, on marine biota, increasing our knowledge of molecular targets involved in responses to environmental stressors.Luisa AlbaranoValerio ZupoMarco GuidaGiovanni LibralatoDavide CaramielloNadia RuoccoMaria CostantiniMDPI AGarticlearomatic hydrocarbonspolychlorinated biphenylssea urchinBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12498, p 12498 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aromatic hydrocarbons polychlorinated biphenyls sea urchin Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	aromatic hydrocarbons polychlorinated biphenyls sea urchin Biology (General) QH301-705.5 Chemistry QD1-999 Luisa Albarano Valerio Zupo Marco Guida Giovanni Libralato Davide Caramiello Nadia Ruocco Maria Costantini PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
description	Polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) represent the most common pollutants in the marine sediments. Previous investigations demonstrated short-term sublethal effects of sediments polluted with both contaminants on the sea urchin <i>Paracentrotus lividus</i> after 2 months of exposure in mesocosms. In particular, morphological malformations observed in <i>P. lividus</i> embryos deriving from adults exposed to PAHs and PCBs were explained at molecular levels by <i>de novo</i> transcriptome assembly and real-time qPCR, leading to the identification of several differentially expressed genes involved in key physiological processes. Here, we extensively explored the genes involved in the response of the sea urchin <i>P. lividus</i> to PAHs and PCBs. Firstly, 25 new genes were identified and interactomic analysis revealed that they were functionally connected among them and to several genes previously defined as molecular targets of response to the two pollutants under analysis. The expression levels of these 25 genes were followed by Real Time qPCR, showing that almost all genes analyzed were affected by PAHs and PCBs. These findings represent an important further step in defining the impacts of slight concentrations of such contaminants on sea urchins and, more in general, on marine biota, increasing our knowledge of molecular targets involved in responses to environmental stressors.
format	article
author	Luisa Albarano Valerio Zupo Marco Guida Giovanni Libralato Davide Caramiello Nadia Ruocco Maria Costantini
author_facet	Luisa Albarano Valerio Zupo Marco Guida Giovanni Libralato Davide Caramiello Nadia Ruocco Maria Costantini
author_sort	Luisa Albarano
title	PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
title_short	PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
title_full	PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
title_fullStr	PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
title_full_unstemmed	PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos
title_sort	pahs and pcbs affect functionally intercorrelated genes in the sea urchin <i>paracentrotus lividus</i> embryos
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/63db73b3b59e496590ed902772bf5fcd
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PAHs and PCBs Affect Functionally Intercorrelated Genes in the Sea Urchin <i>Paracentrotus lividus</i> Embryos

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