Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.

DNA can be concatenated by hybridization of DNA fragments with protruding single-stranded termini. DNA cleavage occurring at a nucleotide containing a DNA base analogue is a useful method to obtain DNA with designed protruding termini. Here, we report a novel non-enzymatic DNA cleavage reaction for...

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Autores principales:	Shuji Ikeda, Kazuki Tainaka, Katsuhiko Matsumoto, Yuta Shinohara, Koji L Ode, Etsuo A Susaki, Hiroki R Ueda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63dd81416da14f2aa85ecc56d944b095
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spelling	oai:doaj.org-article:63dd81416da14f2aa85ecc56d944b0952021-11-18T08:27:12ZNon-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.1932-620310.1371/journal.pone.0092369https://doaj.org/article/63dd81416da14f2aa85ecc56d944b0952014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24647759/?tool=EBIhttps://doaj.org/toc/1932-6203DNA can be concatenated by hybridization of DNA fragments with protruding single-stranded termini. DNA cleavage occurring at a nucleotide containing a DNA base analogue is a useful method to obtain DNA with designed protruding termini. Here, we report a novel non-enzymatic DNA cleavage reaction for DNA concatenation. We found that DNA is cleaved at a nucleotide containing 5-ethynyluracil in a methylamine aqueous solution to generate 5'-phosphorylated DNA fragment as a cleavage product. We demonstrated that the reaction can be applied to DNA concatenation of PCR-amplified DNA fragments. This novel non-enzymatic DNA cleavage reaction is a simple practical approach for DNA concatenation.Shuji IkedaKazuki TainakaKatsuhiko MatsumotoYuta ShinoharaKoji L OdeEtsuo A SusakiHiroki R UedaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92369 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuji Ikeda Kazuki Tainaka Katsuhiko Matsumoto Yuta Shinohara Koji L Ode Etsuo A Susaki Hiroki R Ueda Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
description	DNA can be concatenated by hybridization of DNA fragments with protruding single-stranded termini. DNA cleavage occurring at a nucleotide containing a DNA base analogue is a useful method to obtain DNA with designed protruding termini. Here, we report a novel non-enzymatic DNA cleavage reaction for DNA concatenation. We found that DNA is cleaved at a nucleotide containing 5-ethynyluracil in a methylamine aqueous solution to generate 5'-phosphorylated DNA fragment as a cleavage product. We demonstrated that the reaction can be applied to DNA concatenation of PCR-amplified DNA fragments. This novel non-enzymatic DNA cleavage reaction is a simple practical approach for DNA concatenation.
format	article
author	Shuji Ikeda Kazuki Tainaka Katsuhiko Matsumoto Yuta Shinohara Koji L Ode Etsuo A Susaki Hiroki R Ueda
author_facet	Shuji Ikeda Kazuki Tainaka Katsuhiko Matsumoto Yuta Shinohara Koji L Ode Etsuo A Susaki Hiroki R Ueda
author_sort	Shuji Ikeda
title	Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
title_short	Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
title_full	Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
title_fullStr	Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
title_full_unstemmed	Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.
title_sort	non-enzymatic dna cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to dna concatenation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/63dd81416da14f2aa85ecc56d944b095
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Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation.

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