Tissue-specific expression and regulatory networks of pig microRNAome.

<h4>Background</h4>Despite the economic and medical importance of the pig, knowledge about its genome organization, gene expression regulation, and molecular mechanisms involved in physiological processes is far from that achieved for mouse and rat, the two most used model organisms in b...

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Autores principales:	Paolo Martini, Gabriele Sales, Mattia Brugiolo, Alessandro Gandaglia, Filippo Naso, Cristiano De Pittà, Michele Spina, Gino Gerosa, Francesco Chemello, Chiara Romualdi, Stefano Cagnin, Gerolamo Lanfranchi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63e7d73ac83144978b4e081eeecd8970
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spelling	oai:doaj.org-article:63e7d73ac83144978b4e081eeecd89702021-11-18T08:25:09ZTissue-specific expression and regulatory networks of pig microRNAome.1932-620310.1371/journal.pone.0089755https://doaj.org/article/63e7d73ac83144978b4e081eeecd89702014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24699212/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Despite the economic and medical importance of the pig, knowledge about its genome organization, gene expression regulation, and molecular mechanisms involved in physiological processes is far from that achieved for mouse and rat, the two most used model organisms in biomedical research. MicroRNAs (miRNAs) are a wide class of molecules that exert a recognized role in gene expression modulation, but only 280 miRNAs in pig have been characterized to date.<h4>Results</h4>We applied a novel computational approach to predict species-specific and conserved miRNAs in the pig genome, which were then subjected to experimental validation. We experimentally identified candidate miRNAs sequences grouped in high-confidence (424) and medium-confidence (353) miRNAs according to RNA-seq results. A group of miRNAs was also validated by PCR experiments. We established the subtle variability in expression of isomiRs and miRNA-miRNA star couples supporting a biological function for these molecules. Finally, miRNA and mRNA expression profiles produced from the same sample of 20 different tissue of the animal were combined, using a correlation threshold to filter miRNA-target predictions, to identify tissue-specific regulatory networks.<h4>Conclusions</h4>Our data represent a significant progress in the current understanding of miRNAome in pig. The identification of miRNAs, their target mRNAs, and the construction of regulatory circuits will provide new insights into the complex biological networks in several tissues of this important animal model.Paolo MartiniGabriele SalesMattia BrugioloAlessandro GandagliaFilippo NasoCristiano De PittàMichele SpinaGino GerosaFrancesco ChemelloChiara RomualdiStefano CagninGerolamo LanfranchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e89755 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paolo Martini Gabriele Sales Mattia Brugiolo Alessandro Gandaglia Filippo Naso Cristiano De Pittà Michele Spina Gino Gerosa Francesco Chemello Chiara Romualdi Stefano Cagnin Gerolamo Lanfranchi Tissue-specific expression and regulatory networks of pig microRNAome.
description	<h4>Background</h4>Despite the economic and medical importance of the pig, knowledge about its genome organization, gene expression regulation, and molecular mechanisms involved in physiological processes is far from that achieved for mouse and rat, the two most used model organisms in biomedical research. MicroRNAs (miRNAs) are a wide class of molecules that exert a recognized role in gene expression modulation, but only 280 miRNAs in pig have been characterized to date.<h4>Results</h4>We applied a novel computational approach to predict species-specific and conserved miRNAs in the pig genome, which were then subjected to experimental validation. We experimentally identified candidate miRNAs sequences grouped in high-confidence (424) and medium-confidence (353) miRNAs according to RNA-seq results. A group of miRNAs was also validated by PCR experiments. We established the subtle variability in expression of isomiRs and miRNA-miRNA star couples supporting a biological function for these molecules. Finally, miRNA and mRNA expression profiles produced from the same sample of 20 different tissue of the animal were combined, using a correlation threshold to filter miRNA-target predictions, to identify tissue-specific regulatory networks.<h4>Conclusions</h4>Our data represent a significant progress in the current understanding of miRNAome in pig. The identification of miRNAs, their target mRNAs, and the construction of regulatory circuits will provide new insights into the complex biological networks in several tissues of this important animal model.
format	article
author	Paolo Martini Gabriele Sales Mattia Brugiolo Alessandro Gandaglia Filippo Naso Cristiano De Pittà Michele Spina Gino Gerosa Francesco Chemello Chiara Romualdi Stefano Cagnin Gerolamo Lanfranchi
author_facet	Paolo Martini Gabriele Sales Mattia Brugiolo Alessandro Gandaglia Filippo Naso Cristiano De Pittà Michele Spina Gino Gerosa Francesco Chemello Chiara Romualdi Stefano Cagnin Gerolamo Lanfranchi
author_sort	Paolo Martini
title	Tissue-specific expression and regulatory networks of pig microRNAome.
title_short	Tissue-specific expression and regulatory networks of pig microRNAome.
title_full	Tissue-specific expression and regulatory networks of pig microRNAome.
title_fullStr	Tissue-specific expression and regulatory networks of pig microRNAome.
title_full_unstemmed	Tissue-specific expression and regulatory networks of pig microRNAome.
title_sort	tissue-specific expression and regulatory networks of pig micrornaome.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/63e7d73ac83144978b4e081eeecd8970
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_version_	1718421805422608384

Tissue-specific expression and regulatory networks of pig microRNAome.

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