Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns

Abstract Earlier we have reported mercury-induced alterations in functional dynamics of buck spermatozoa through free radicals-mediated oxidative stress and spontaneous acrosome reaction. Based on our earlier findings, we aimed to investigate the effect of mercury exposure on motility, kinematic pat...

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Autores principales:	Bhawna Kushawaha, Rajkumar Singh Yadav, Dilip Kumar Swain, Priyambada Kumari, Akhilesh Kumar, Brijesh Yadav, Mukul Anand, Sarvajeet Yadav, Dipty Singh, Satish Kumar Garg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63e86f807f2042428466d505a661b500
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spelling	oai:doaj.org-article:63e86f807f2042428466d505a661b5002021-12-02T14:01:24ZCollapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns10.1038/s41598-020-80235-y2045-2322https://doaj.org/article/63e86f807f2042428466d505a661b5002021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80235-yhttps://doaj.org/toc/2045-2322Abstract Earlier we have reported mercury-induced alterations in functional dynamics of buck spermatozoa through free radicals-mediated oxidative stress and spontaneous acrosome reaction. Based on our earlier findings, we aimed to investigate the effect of mercury exposure on motility, kinematic patterns, DNA damage, apoptosis and ultra-structural alterations in goat spermatozoa following in vitro exposure to different concentrations (0.031–1.25 µg/ml) of mercuric chloride for 15 min and 3 h. Following exposure of sperm cells to 0.031 µg/ml of mercuric chloride for 3 h, livability and motility of sperms was significantly reduced along with altered kinematic patterns, significant increase in per cent necrotic sperm cells and number of cells showing DNA damage; and this effect was dose- and time-dependent. Contrary to up-regulation of Bax gene after 3 h in control group, there was significant increase in expression of Bcl-2 in mercury-treated groups. Transmission electron microscopy studies revealed rifts and nicks in plasma and acrosomal membrane, mitochondrial sheath, and collapsed mitochondria with loss of helical organization of mitochondria in the middle piece of spermatozoa. Our findings evidently suggest that mercury induces necrosis instead of apoptosis and targets the membrane, acrosome, mid piece of sperms; and the damage to mitochondria seems to be responsible for alterations in functional and kinematic attributes of spermatozoa.Bhawna KushawahaRajkumar Singh YadavDilip Kumar SwainPriyambada KumariAkhilesh KumarBrijesh YadavMukul AnandSarvajeet YadavDipty SinghSatish Kumar GargNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bhawna Kushawaha Rajkumar Singh Yadav Dilip Kumar Swain Priyambada Kumari Akhilesh Kumar Brijesh Yadav Mukul Anand Sarvajeet Yadav Dipty Singh Satish Kumar Garg Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
description	Abstract Earlier we have reported mercury-induced alterations in functional dynamics of buck spermatozoa through free radicals-mediated oxidative stress and spontaneous acrosome reaction. Based on our earlier findings, we aimed to investigate the effect of mercury exposure on motility, kinematic patterns, DNA damage, apoptosis and ultra-structural alterations in goat spermatozoa following in vitro exposure to different concentrations (0.031–1.25 µg/ml) of mercuric chloride for 15 min and 3 h. Following exposure of sperm cells to 0.031 µg/ml of mercuric chloride for 3 h, livability and motility of sperms was significantly reduced along with altered kinematic patterns, significant increase in per cent necrotic sperm cells and number of cells showing DNA damage; and this effect was dose- and time-dependent. Contrary to up-regulation of Bax gene after 3 h in control group, there was significant increase in expression of Bcl-2 in mercury-treated groups. Transmission electron microscopy studies revealed rifts and nicks in plasma and acrosomal membrane, mitochondrial sheath, and collapsed mitochondria with loss of helical organization of mitochondria in the middle piece of spermatozoa. Our findings evidently suggest that mercury induces necrosis instead of apoptosis and targets the membrane, acrosome, mid piece of sperms; and the damage to mitochondria seems to be responsible for alterations in functional and kinematic attributes of spermatozoa.
format	article
author	Bhawna Kushawaha Rajkumar Singh Yadav Dilip Kumar Swain Priyambada Kumari Akhilesh Kumar Brijesh Yadav Mukul Anand Sarvajeet Yadav Dipty Singh Satish Kumar Garg
author_facet	Bhawna Kushawaha Rajkumar Singh Yadav Dilip Kumar Swain Priyambada Kumari Akhilesh Kumar Brijesh Yadav Mukul Anand Sarvajeet Yadav Dipty Singh Satish Kumar Garg
author_sort	Bhawna Kushawaha
title	Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
title_short	Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
title_full	Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
title_fullStr	Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
title_full_unstemmed	Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
title_sort	collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63e86f807f2042428466d505a661b500
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Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns

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