Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota

Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota

Bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with flow cytometry (FACS) has been utilized to profile active microbiome of environmental samples. Here, the authors combine BONCAT-FACS and 16 S rRNA sequencing to characterize the translational activity of bacterial communities withi...

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Autores principales: Talia D. Valentini, Sarah K. Lucas, Kelsey A. Binder, Lydia C. Cameron, Jason A. Motl, Jordan M. Dunitz, Ryan C. Hunter
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/63e92ae2f59b44fdbdff84eaf0427dda
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