Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

Abstract Influenza virus and coronavirus, belonging to enveloped RNA viruses, are major causes of human respiratory diseases. The aim of this study was to investigate the broad spectrum antiviral activity of a naturally existing sulfated polysaccharide, lambda-carrageenan (λ-CGN), purified from mari...

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Autores principales:	Yejin Jang, Heegwon Shin, Myoung Kyu Lee, Oh Seung Kwon, Jin Soo Shin, Yong-il Kim, Chan Woo Kim, Hye-Ra Lee, Meehyein Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63ecc43f116343af965dc935ef0e68a7
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spelling	oai:doaj.org-article:63ecc43f116343af965dc935ef0e68a72021-12-02T14:12:09ZAntiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 210.1038/s41598-020-80896-92045-2322https://doaj.org/article/63ecc43f116343af965dc935ef0e68a72021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80896-9https://doaj.org/toc/2045-2322Abstract Influenza virus and coronavirus, belonging to enveloped RNA viruses, are major causes of human respiratory diseases. The aim of this study was to investigate the broad spectrum antiviral activity of a naturally existing sulfated polysaccharide, lambda-carrageenan (λ-CGN), purified from marine red algae. Cell culture-based assays revealed that the macromolecule efficiently inhibited both influenza A and B viruses with EC50 values ranging from 0.3 to 1.4 μg/ml, as well as currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with an EC50 value of 0.9 ± 1.1 μg/ml. No toxicity to the host cells was observed at concentrations up to 300 μg/ml. Plaque titration and western blot analysis verified that λ-CGN reduced expression of viral proteins in cell lysates and suppressed progeny virus production in culture supernatants in a dose-dependent manner. This polyanionic compound exerts antiviral activity by targeting viral attachment to cell surface receptors and preventing virus entry. Moreover, its intranasal administration to mice during influenza A viral challenge not only alleviated infection-mediated reductions in body weight but also protected 60% of mice from virus-induced mortality. Thus, λ-CGN could be a promising antiviral agent for preventing infection with several respiratory viruses.Yejin JangHeegwon ShinMyoung Kyu LeeOh Seung KwonJin Soo ShinYong-il KimChan Woo KimHye-Ra LeeMeehyein KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yejin Jang Heegwon Shin Myoung Kyu Lee Oh Seung Kwon Jin Soo Shin Yong-il Kim Chan Woo Kim Hye-Ra Lee Meehyein Kim Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
description	Abstract Influenza virus and coronavirus, belonging to enveloped RNA viruses, are major causes of human respiratory diseases. The aim of this study was to investigate the broad spectrum antiviral activity of a naturally existing sulfated polysaccharide, lambda-carrageenan (λ-CGN), purified from marine red algae. Cell culture-based assays revealed that the macromolecule efficiently inhibited both influenza A and B viruses with EC50 values ranging from 0.3 to 1.4 μg/ml, as well as currently circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with an EC50 value of 0.9 ± 1.1 μg/ml. No toxicity to the host cells was observed at concentrations up to 300 μg/ml. Plaque titration and western blot analysis verified that λ-CGN reduced expression of viral proteins in cell lysates and suppressed progeny virus production in culture supernatants in a dose-dependent manner. This polyanionic compound exerts antiviral activity by targeting viral attachment to cell surface receptors and preventing virus entry. Moreover, its intranasal administration to mice during influenza A viral challenge not only alleviated infection-mediated reductions in body weight but also protected 60% of mice from virus-induced mortality. Thus, λ-CGN could be a promising antiviral agent for preventing infection with several respiratory viruses.
format	article
author	Yejin Jang Heegwon Shin Myoung Kyu Lee Oh Seung Kwon Jin Soo Shin Yong-il Kim Chan Woo Kim Hye-Ra Lee Meehyein Kim
author_facet	Yejin Jang Heegwon Shin Myoung Kyu Lee Oh Seung Kwon Jin Soo Shin Yong-il Kim Chan Woo Kim Hye-Ra Lee Meehyein Kim
author_sort	Yejin Jang
title	Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
title_short	Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
title_full	Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
title_fullStr	Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
title_full_unstemmed	Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
title_sort	antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63ecc43f116343af965dc935ef0e68a7
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Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

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