Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken

Abstract Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of...

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Autores principales:	Hafiz I. Hussain, Zahid Iqbal, Mohamed N. Seleem, Deyu Huang, Adeel Sattar, Haihong Hao, Zonghui Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63ee2130b49a4a8fb98f97cb0eb7ea1c
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spelling	oai:doaj.org-article:63ee2130b49a4a8fb98f97cb0eb7ea1c2021-12-02T16:06:38ZVirulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken10.1038/s41598-017-07798-12045-2322https://doaj.org/article/63ee2130b49a4a8fb98f97cb0eb7ea1c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07798-1https://doaj.org/toc/2045-2322Abstract Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.Hafiz I. HussainZahid IqbalMohamed N. SeleemDeyu HuangAdeel SattarHaihong HaoZonghui YuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hafiz I. Hussain Zahid Iqbal Mohamed N. Seleem Deyu Huang Adeel Sattar Haihong Hao Zonghui Yuan Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
description	Abstract Numerous studies have examined the prevalence of pathogenic Escherichia coli in poultry and poultry products; however, limited data are available regarding their resistance- and virulence-associated gene expression profiles. This study was designed to examine the resistance and virulence of poultry E. coli strains in vitro and in vivo via antibiotic susceptibility, biofilm formation and adhesion, and invasion and intracellular survivability assays in Caco-2 and Raw 264.7 cell lines as well as the determination of the median lethal dose in two-day old chickens. A clinical pathogenic multidrug-resistant isolate, E. coli 381, isolated from broilers, was found to be highly virulent in cell culture and 1000-fold more virulent in a chicken model than other strains; accordingly, the isolate was subsequently selected for transcriptome analysis. The comparative gene expression profile of MDR E. coli 381 and the reference human strain E. coli ATCC 25922 was completed with Illumina HiSeq. 2500 transcriptome analysis. Differential gene expression analysis indicates that there are multiple pathways involved in the resistance and virulence of this highly virulent strain. The results garnered from this study provide critical information about the highly virulent MDR E. coli strain of poultry origin and warrant further investigation due to its significant threat to public health.
format	article
author	Hafiz I. Hussain Zahid Iqbal Mohamed N. Seleem Deyu Huang Adeel Sattar Haihong Hao Zonghui Yuan
author_facet	Hafiz I. Hussain Zahid Iqbal Mohamed N. Seleem Deyu Huang Adeel Sattar Haihong Hao Zonghui Yuan
author_sort	Hafiz I. Hussain
title	Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
title_short	Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
title_full	Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
title_fullStr	Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
title_full_unstemmed	Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken
title_sort	virulence and transcriptome profile of multidrug-resistant escherichia coli from chicken
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63ee2130b49a4a8fb98f97cb0eb7ea1c
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Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken

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