Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.

A new secretion system, called the Type VI Secretion system (T6SS), was recently reported in Vibrio cholerae, Pseudomonas aeruginosa and Burkholderia mallei. A total of 18 genes have been identified to be belonging to this secretion system in V. cholerae. Here we attempt to identify presence of T6SS...

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Autores principales:	Sakshi Shrivastava, Sharmila S Mande
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63f0a786697f4505a393bc3a71d6b23b
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spelling	oai:doaj.org-article:63f0a786697f4505a393bc3a71d6b23b2021-11-25T06:11:13ZIdentification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.1932-620310.1371/journal.pone.0002955https://doaj.org/article/63f0a786697f4505a393bc3a71d6b23b2008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18698408/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A new secretion system, called the Type VI Secretion system (T6SS), was recently reported in Vibrio cholerae, Pseudomonas aeruginosa and Burkholderia mallei. A total of 18 genes have been identified to be belonging to this secretion system in V. cholerae. Here we attempt to identify presence of T6SS in other bacterial genomes. This includes identification of orthologous sequences, conserved motifs, domains, families, 3D folds, genomic islands containing T6SS components, phylogenetic profiles and protein-protein association of these components. Our analysis indicates presence of T6SS in 42 bacteria and its absence in most of their non-pathogenic species, suggesting the role of T6SS in imparting pathogenicity to an organism. Analysis of genomic regions containing T6SS components, phylogenetic profiles and protein-protein association of T6SS components indicate few additional genes which could be involved in this secretion system. Based on our studies, functional annotations were assigned to most of the components. Except one of the genes, we could group all the other genes of T6SS into those belonging to the puncturing device, and those located in the outer membrane, transmembrane and inner membrane. Based on our analysis, we have proposed a model of T6SS and have compared the same with the other bacterial secretion systems.Sakshi ShrivastavaSharmila S MandePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2955 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sakshi Shrivastava Sharmila S Mande Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
description	A new secretion system, called the Type VI Secretion system (T6SS), was recently reported in Vibrio cholerae, Pseudomonas aeruginosa and Burkholderia mallei. A total of 18 genes have been identified to be belonging to this secretion system in V. cholerae. Here we attempt to identify presence of T6SS in other bacterial genomes. This includes identification of orthologous sequences, conserved motifs, domains, families, 3D folds, genomic islands containing T6SS components, phylogenetic profiles and protein-protein association of these components. Our analysis indicates presence of T6SS in 42 bacteria and its absence in most of their non-pathogenic species, suggesting the role of T6SS in imparting pathogenicity to an organism. Analysis of genomic regions containing T6SS components, phylogenetic profiles and protein-protein association of T6SS components indicate few additional genes which could be involved in this secretion system. Based on our studies, functional annotations were assigned to most of the components. Except one of the genes, we could group all the other genes of T6SS into those belonging to the puncturing device, and those located in the outer membrane, transmembrane and inner membrane. Based on our analysis, we have proposed a model of T6SS and have compared the same with the other bacterial secretion systems.
format	article
author	Sakshi Shrivastava Sharmila S Mande
author_facet	Sakshi Shrivastava Sharmila S Mande
author_sort	Sakshi Shrivastava
title	Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
title_short	Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
title_full	Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
title_fullStr	Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
title_full_unstemmed	Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.
title_sort	identification and functional characterization of gene components of type vi secretion system in bacterial genomes.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/63f0a786697f4505a393bc3a71d6b23b
work_keys_str_mv	AT sakshishrivastava identificationandfunctionalcharacterizationofgenecomponentsoftypevisecretionsysteminbacterialgenomes AT sharmilasmande identificationandfunctionalcharacterizationofgenecomponentsoftypevisecretionsysteminbacterialgenomes
_version_	1718414045227253760

Identification and functional characterization of gene components of Type VI Secretion system in bacterial genomes.

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