Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice

Abstract The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lindsey B. Crawford, Rebecca Tempel, Daniel N. Streblow, Craig Kreklywich, Patricia Smith, Louis J. Picker, Jay A. Nelson, Patrizia Caposio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63f137c8af204eeab7c5949faeb8e663
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:63f137c8af204eeab7c5949faeb8e663
record_format	dspace
spelling	oai:doaj.org-article:63f137c8af204eeab7c5949faeb8e6632021-12-02T16:08:00ZHuman Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice10.1038/s41598-017-01051-52045-2322https://doaj.org/article/63f137c8af204eeab7c5949faeb8e6632017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01051-5https://doaj.org/toc/2045-2322Abstract The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mice (huNSG) results in viral latency that can be reactivated following G-CSF treatment. In this study, we characterized the functional human adaptive immune responses in HCMV latently-infected huBLT (humanized Bone marrow-Liver-Thymus) mice. Following infection, huBLT mice generate human effector and central memory CD4+ and CD8+ T-cell responses reactive to peptides corresponding to both IE and pp65 proteins. Additionally, both HCMV specific IgM and IgG B-cell responses with the ability to neutralize virus were detected. These results indicate that the HCMV huBLT mouse model may provide a valuable tool to study viral latency and reactivation as well as evaluate HCMV vaccines and immune responses in the context of a functional human immune system.Lindsey B. CrawfordRebecca TempelDaniel N. StreblowCraig KreklywichPatricia SmithLouis J. PickerJay A. NelsonPatrizia CaposioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lindsey B. Crawford Rebecca Tempel Daniel N. Streblow Craig Kreklywich Patricia Smith Louis J. Picker Jay A. Nelson Patrizia Caposio Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
description	Abstract The strict species specificity of Human Cytomegalovirus (HCMV) has impeded our understanding of antiviral adaptive immune responses in the context of a human immune system. We have previously shown that HCMV infection of human hematopoietic progenitor cells engrafted in immune deficient mice (huNSG) results in viral latency that can be reactivated following G-CSF treatment. In this study, we characterized the functional human adaptive immune responses in HCMV latently-infected huBLT (humanized Bone marrow-Liver-Thymus) mice. Following infection, huBLT mice generate human effector and central memory CD4+ and CD8+ T-cell responses reactive to peptides corresponding to both IE and pp65 proteins. Additionally, both HCMV specific IgM and IgG B-cell responses with the ability to neutralize virus were detected. These results indicate that the HCMV huBLT mouse model may provide a valuable tool to study viral latency and reactivation as well as evaluate HCMV vaccines and immune responses in the context of a functional human immune system.
format	article
author	Lindsey B. Crawford Rebecca Tempel Daniel N. Streblow Craig Kreklywich Patricia Smith Louis J. Picker Jay A. Nelson Patrizia Caposio
author_facet	Lindsey B. Crawford Rebecca Tempel Daniel N. Streblow Craig Kreklywich Patricia Smith Louis J. Picker Jay A. Nelson Patrizia Caposio
author_sort	Lindsey B. Crawford
title	Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
title_short	Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
title_full	Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
title_fullStr	Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
title_full_unstemmed	Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice
title_sort	human cytomegalovirus induces cellular and humoral virus-specific immune responses in humanized blt mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63f137c8af204eeab7c5949faeb8e663
work_keys_str_mv	AT lindseybcrawford humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT rebeccatempel humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT danielnstreblow humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT craigkreklywich humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT patriciasmith humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT louisjpicker humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT jayanelson humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice AT patriziacaposio humancytomegalovirusinducescellularandhumoralvirusspecificimmuneresponsesinhumanizedbltmice
_version_	1718384659613614080

Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice

Ejemplares similares