TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust

Members of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types of stresses. To study the function of TaMYB29 transcription factor—a member of the R2R3-MYB superfamily—in response to an avirulent...

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Autores principales:	Xiaoxu Zhu, Xiang Li, Qi He, Dongxiao Guo, Caiqi Liu, Junying Cao, Zhongyi Wu, Zhensheng Kang, Xiaojing Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	TaMYB29 transcription factor reactive oxygen species hypersensitive response stripe rust VIGS Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/63fad5107a494a71bbb264a78169093d
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spelling	oai:doaj.org-article:63fad5107a494a71bbb264a78169093d2021-12-01T13:07:25ZTaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust1664-462X10.3389/fpls.2021.783388https://doaj.org/article/63fad5107a494a71bbb264a78169093d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.783388/fullhttps://doaj.org/toc/1664-462XMembers of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types of stresses. To study the function of TaMYB29 transcription factor—a member of the R2R3-MYB superfamily—in response to an avirulent race of stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), we identified and cloned the TaMYB29 gene from wheat cultivar (cv.) AvS+Yr10 following infection with Pst. The TaMYB29 protein, comprising 261 amino acids, contains two highly conserved MYB domains. We first showed that TaMYB29 is a transcription factor, whose transcriptional levels are significantly induced by salicylic acid (SA), abscisic acid (ABA), jasmonic acid (JA), ethylene (ET), and Pst. The results showed that TaMYB29 is involved in the wheat response to stipe rust. The overexpression of the TaMYB29 gene resulted in the accumulation of reactive oxygen species (ROS) and pathogen-independent cell death in Nicotiana benthamiana leaves. The silencing of TaMYB29 gene in wheat cv. AvS+Yr10, containing the stripe rust resistance gene Yr10, promoted hyphae growth, significantly downregulated the expression of pathogenesis-related (PR) genes, and substantially reduced the wheat resistance to Pst compared with the non-silenced control. In addition, the accumulation of hydrogen peroxide (H2O2) significantly decreased, and the activity of catalase, an enzyme required for H2O2 scavenging, was elevated. Altogether, TaMYB29 positively regulates the defense response against stripe rust in wheat AvS+Yr10 by enhancing H2O2 accumulation, PR gene expression, and SA signaling pathway-induced cell death. These results provide new insights into the contribution of TaMYB29 to the defense response against rust pathogens in wheat.Xiaoxu ZhuXiaoxu ZhuXiang LiQi HeDongxiao GuoCaiqi LiuJunying CaoZhongyi WuZhensheng KangXiaojing WangFrontiers Media S.A.articleTaMYB29transcription factorreactive oxygen specieshypersensitive responsestripe rustVIGSPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TaMYB29 transcription factor reactive oxygen species hypersensitive response stripe rust VIGS Plant culture SB1-1110
spellingShingle	TaMYB29 transcription factor reactive oxygen species hypersensitive response stripe rust VIGS Plant culture SB1-1110 Xiaoxu Zhu Xiaoxu Zhu Xiang Li Qi He Dongxiao Guo Caiqi Liu Junying Cao Zhongyi Wu Zhensheng Kang Xiaojing Wang TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
description	Members of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types of stresses. To study the function of TaMYB29 transcription factor—a member of the R2R3-MYB superfamily—in response to an avirulent race of stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), we identified and cloned the TaMYB29 gene from wheat cultivar (cv.) AvS+Yr10 following infection with Pst. The TaMYB29 protein, comprising 261 amino acids, contains two highly conserved MYB domains. We first showed that TaMYB29 is a transcription factor, whose transcriptional levels are significantly induced by salicylic acid (SA), abscisic acid (ABA), jasmonic acid (JA), ethylene (ET), and Pst. The results showed that TaMYB29 is involved in the wheat response to stipe rust. The overexpression of the TaMYB29 gene resulted in the accumulation of reactive oxygen species (ROS) and pathogen-independent cell death in Nicotiana benthamiana leaves. The silencing of TaMYB29 gene in wheat cv. AvS+Yr10, containing the stripe rust resistance gene Yr10, promoted hyphae growth, significantly downregulated the expression of pathogenesis-related (PR) genes, and substantially reduced the wheat resistance to Pst compared with the non-silenced control. In addition, the accumulation of hydrogen peroxide (H2O2) significantly decreased, and the activity of catalase, an enzyme required for H2O2 scavenging, was elevated. Altogether, TaMYB29 positively regulates the defense response against stripe rust in wheat AvS+Yr10 by enhancing H2O2 accumulation, PR gene expression, and SA signaling pathway-induced cell death. These results provide new insights into the contribution of TaMYB29 to the defense response against rust pathogens in wheat.
format	article
author	Xiaoxu Zhu Xiaoxu Zhu Xiang Li Qi He Dongxiao Guo Caiqi Liu Junying Cao Zhongyi Wu Zhensheng Kang Xiaojing Wang
author_facet	Xiaoxu Zhu Xiaoxu Zhu Xiang Li Qi He Dongxiao Guo Caiqi Liu Junying Cao Zhongyi Wu Zhensheng Kang Xiaojing Wang
author_sort	Xiaoxu Zhu
title	TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
title_short	TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
title_full	TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
title_fullStr	TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
title_full_unstemmed	TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
title_sort	tamyb29: a novel r2r3-myb transcription factor involved in wheat defense against stripe rust
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/63fad5107a494a71bbb264a78169093d
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TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust

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