Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

Abstract. Yurnaliza Y, Jamilah I, Hartanto A, Lutfia A. 2021. Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides. Biodiversitas 22: 1467-1473. Exopolysaccharides (EPS) are microbial polysaccharides with a high biotechnological application in many fields d...

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Autores principales:	Yurnaliza Yurnaliza, It Jamilah, Adrian Hartanto, Anisa Lutfia
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	ascomycetes endophytes exopolysaccharides oil palm pestalotiopsis annulohypoxylon thailandicum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/63fe43866ad540bf971e825d2ae6f439
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spelling	oai:doaj.org-article:63fe43866ad540bf971e825d2ae6f4392021-11-22T00:56:36ZScreening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides1412-033X2085-472210.13057/biodiv/d220350https://doaj.org/article/63fe43866ad540bf971e825d2ae6f4392021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7879https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yurnaliza Y, Jamilah I, Hartanto A, Lutfia A. 2021. Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides. Biodiversitas 22: 1467-1473. Exopolysaccharides (EPS) are microbial polysaccharides with a high biotechnological application in many fields due to their bioactivity and biocompatibility. Endophytic fungi are potential agents for producing EPS even though they remained under-exploited, especially those originating as indigenous strains from oil palm (Elaeis guineensis Jacq.). The present study reports on EPS production by a collection of endophytic fungi isolated from the petioles of oil palm under submerged fermentation. The culture medium was formulated with sucrose as C-source to trigger the secretion of EPS by the strains. Isolation of EPS was done through absolute ethanol precipitation in cold conditions. A total of 29 endophytic fungal strains was recovered from the foliar part of the plants. Molecular identification based on ITS-rDNA region and phylogenetic construction of each lineage revealed the dominant species from Pestalotiopsidaceae (syn. Amphisphaeriaceae), followed by Hypoxylaceae, Pleosporaceae, Didymosphaeriaceae, Sporocadaceae, and other minor families. In general, most isolates were ascomycetous fungi along with a basidiomycetous and zygomycetous fungus. After fermentation for 10 days, three fungal endophytes identified as Annulohypoxylon thailandicum P8 (Hypoxylaceae), Pseudopestalotiopsis simitheae P1 (Pestalotiopsidaceae), and Diaporthe eucalyptorum P11 (Diaporthaceae) secreted EPS higher in values than the others. The most prominent strain was A. thailandicum P8 with a yield of >50 mg/100 mL of filtrate solution. Based on FT-IR spectral analysis, the EPS product was detected to contain carboxyl, carbonyl, glycosidic bonds, and hydroxyl groups hereafter confirmed the characteristics of exopolysaccharides. The results demonstrated that the EPS may be evaluated for its bioactivity in a future study.Yurnaliza YurnalizaIt JamilahAdrian HartantoAnisa LutfiaMBI & UNS Soloarticleascomycetesendophytesexopolysaccharidesoil palmpestalotiopsisannulohypoxylon thailandicumBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ascomycetes endophytes exopolysaccharides oil palm pestalotiopsis annulohypoxylon thailandicum Biology (General) QH301-705.5
spellingShingle	ascomycetes endophytes exopolysaccharides oil palm pestalotiopsis annulohypoxylon thailandicum Biology (General) QH301-705.5 Yurnaliza Yurnaliza It Jamilah Adrian Hartanto Anisa Lutfia Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
description	Abstract. Yurnaliza Y, Jamilah I, Hartanto A, Lutfia A. 2021. Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides. Biodiversitas 22: 1467-1473. Exopolysaccharides (EPS) are microbial polysaccharides with a high biotechnological application in many fields due to their bioactivity and biocompatibility. Endophytic fungi are potential agents for producing EPS even though they remained under-exploited, especially those originating as indigenous strains from oil palm (Elaeis guineensis Jacq.). The present study reports on EPS production by a collection of endophytic fungi isolated from the petioles of oil palm under submerged fermentation. The culture medium was formulated with sucrose as C-source to trigger the secretion of EPS by the strains. Isolation of EPS was done through absolute ethanol precipitation in cold conditions. A total of 29 endophytic fungal strains was recovered from the foliar part of the plants. Molecular identification based on ITS-rDNA region and phylogenetic construction of each lineage revealed the dominant species from Pestalotiopsidaceae (syn. Amphisphaeriaceae), followed by Hypoxylaceae, Pleosporaceae, Didymosphaeriaceae, Sporocadaceae, and other minor families. In general, most isolates were ascomycetous fungi along with a basidiomycetous and zygomycetous fungus. After fermentation for 10 days, three fungal endophytes identified as Annulohypoxylon thailandicum P8 (Hypoxylaceae), Pseudopestalotiopsis simitheae P1 (Pestalotiopsidaceae), and Diaporthe eucalyptorum P11 (Diaporthaceae) secreted EPS higher in values than the others. The most prominent strain was A. thailandicum P8 with a yield of >50 mg/100 mL of filtrate solution. Based on FT-IR spectral analysis, the EPS product was detected to contain carboxyl, carbonyl, glycosidic bonds, and hydroxyl groups hereafter confirmed the characteristics of exopolysaccharides. The results demonstrated that the EPS may be evaluated for its bioactivity in a future study.
format	article
author	Yurnaliza Yurnaliza It Jamilah Adrian Hartanto Anisa Lutfia
author_facet	Yurnaliza Yurnaliza It Jamilah Adrian Hartanto Anisa Lutfia
author_sort	Yurnaliza Yurnaliza
title	Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
title_short	Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
title_full	Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
title_fullStr	Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
title_full_unstemmed	Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides
title_sort	screening of endophytic fungi from oil palm (elaeis guineensis) in producing exopolysaccharides
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/63fe43866ad540bf971e825d2ae6f439
work_keys_str_mv	AT yurnalizayurnaliza screeningofendophyticfungifromoilpalmelaeisguineensisinproducingexopolysaccharides AT itjamilah screeningofendophyticfungifromoilpalmelaeisguineensisinproducingexopolysaccharides AT adrianhartanto screeningofendophyticfungifromoilpalmelaeisguineensisinproducingexopolysaccharides AT anisalutfia screeningofendophyticfungifromoilpalmelaeisguineensisinproducingexopolysaccharides
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Screening of endophytic fungi from oil palm (Elaeis guineensis) in producing exopolysaccharides

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