The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence
The gut microbiota is believed to play a significant role in psychological and gastrointestinal symptoms in heroin addicts. However, the underlying mechanism remains largely unknown. We show here that heroin addicts had a decrease in body mass index (BMI) and abnormal serum D-lactic acid (DLA), endo...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:64129590ad5d450eb5d63daf2c89bf642021-11-04T09:23:41ZThe Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence2296-861X10.3389/fnut.2021.765414https://doaj.org/article/64129590ad5d450eb5d63daf2c89bf642021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.765414/fullhttps://doaj.org/toc/2296-861XThe gut microbiota is believed to play a significant role in psychological and gastrointestinal symptoms in heroin addicts. However, the underlying mechanism remains largely unknown. We show here that heroin addicts had a decrease in body mass index (BMI) and abnormal serum D-lactic acid (DLA), endotoxin (ET) and diamine oxidase (DAO) levels during their withdrawal stage, suggesting a potential intestinal injury. The gut microbial profiles in the mouse model with heroin dependence showed slightly decreased alpha diversity, as well as higher levels of Bifidobacterium and Sutterella and a decrease in Akkermansia at genus level compared to the control group. Fecal microbiota transplantation (FMT) further confirmed that the microbiota altered by heroin dependence was sufficient to impair body weight and intestinal mucosal barrier integrity in recipient mice. Moreover, short-chain fatty acids (SCFAs) profiling revealed that microbiota-derived propionic acid significantly decreased in heroin dependent mice compared to controls. Overall, our study shows that heroin dependence significantly altered gut microbiota and impaired intestinal mucosal barrier integrity in mice, highlighting the role of the gut microbiota in substance use disorders and the pathophysiology of withdrawal symptoms.Jiqing YangJiqing YangJiqing YangJiqing YangPu XiongPu XiongLing BaiZunyue ZhangZunyue ZhangYong ZhouYong ZhouCheng ChenCheng ChenZhenrong XieZhenrong XieYu XuYu XuMinghui ChenMinghui ChenHuawei WangMei ZhuMei ZhuJuehua YuJuehua YuKunhua WangKunhua WangKunhua WangFrontiers Media S.A.articleheroin dependencegut microbiotaintestinal mucosal barrier integritySCFAsmiceNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021) |
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| topic |
heroin dependence gut microbiota intestinal mucosal barrier integrity SCFAs mice Nutrition. Foods and food supply TX341-641 |
| spellingShingle |
heroin dependence gut microbiota intestinal mucosal barrier integrity SCFAs mice Nutrition. Foods and food supply TX341-641 Jiqing Yang Jiqing Yang Jiqing Yang Jiqing Yang Pu Xiong Pu Xiong Ling Bai Zunyue Zhang Zunyue Zhang Yong Zhou Yong Zhou Cheng Chen Cheng Chen Zhenrong Xie Zhenrong Xie Yu Xu Yu Xu Minghui Chen Minghui Chen Huawei Wang Mei Zhu Mei Zhu Juehua Yu Juehua Yu Kunhua Wang Kunhua Wang Kunhua Wang The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| description |
The gut microbiota is believed to play a significant role in psychological and gastrointestinal symptoms in heroin addicts. However, the underlying mechanism remains largely unknown. We show here that heroin addicts had a decrease in body mass index (BMI) and abnormal serum D-lactic acid (DLA), endotoxin (ET) and diamine oxidase (DAO) levels during their withdrawal stage, suggesting a potential intestinal injury. The gut microbial profiles in the mouse model with heroin dependence showed slightly decreased alpha diversity, as well as higher levels of Bifidobacterium and Sutterella and a decrease in Akkermansia at genus level compared to the control group. Fecal microbiota transplantation (FMT) further confirmed that the microbiota altered by heroin dependence was sufficient to impair body weight and intestinal mucosal barrier integrity in recipient mice. Moreover, short-chain fatty acids (SCFAs) profiling revealed that microbiota-derived propionic acid significantly decreased in heroin dependent mice compared to controls. Overall, our study shows that heroin dependence significantly altered gut microbiota and impaired intestinal mucosal barrier integrity in mice, highlighting the role of the gut microbiota in substance use disorders and the pathophysiology of withdrawal symptoms. |
| format |
article |
| author |
Jiqing Yang Jiqing Yang Jiqing Yang Jiqing Yang Pu Xiong Pu Xiong Ling Bai Zunyue Zhang Zunyue Zhang Yong Zhou Yong Zhou Cheng Chen Cheng Chen Zhenrong Xie Zhenrong Xie Yu Xu Yu Xu Minghui Chen Minghui Chen Huawei Wang Mei Zhu Mei Zhu Juehua Yu Juehua Yu Kunhua Wang Kunhua Wang Kunhua Wang |
| author_facet |
Jiqing Yang Jiqing Yang Jiqing Yang Jiqing Yang Pu Xiong Pu Xiong Ling Bai Zunyue Zhang Zunyue Zhang Yong Zhou Yong Zhou Cheng Chen Cheng Chen Zhenrong Xie Zhenrong Xie Yu Xu Yu Xu Minghui Chen Minghui Chen Huawei Wang Mei Zhu Mei Zhu Juehua Yu Juehua Yu Kunhua Wang Kunhua Wang Kunhua Wang |
| author_sort |
Jiqing Yang |
| title |
The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| title_short |
The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| title_full |
The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| title_fullStr |
The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| title_full_unstemmed |
The Association of Altered Gut Microbiota and Intestinal Mucosal Barrier Integrity in Mice With Heroin Dependence |
| title_sort |
association of altered gut microbiota and intestinal mucosal barrier integrity in mice with heroin dependence |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/64129590ad5d450eb5d63daf2c89bf64 |
| work_keys_str_mv |
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