EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS

Macrophages play a key role in triggering and regulation of neuroregeneration. The characteristic feature of macrophages is pronounced plasticity, which manifests itself in the ability of macrophages to change their functional phenotype depending on the micromilieu. Apoptotic cell clearance (efferoc...

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Autores principales:	I. M. Rashchupkin, E. Ya. Shevela, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	macrophages m2 phenotype sh-sy5y cells differentiation retinoic acid neuroregeneration Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6415eacb459e485a861d532dd7a8f240
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spelling	oai:doaj.org-article:6415eacb459e485a861d532dd7a8f2402021-11-18T08:03:51ZEFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS1563-06252313-741X10.15789/1563-0625-EOM-2276https://doaj.org/article/6415eacb459e485a861d532dd7a8f2402021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2416https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XMacrophages play a key role in triggering and regulation of neuroregeneration. The characteristic feature of macrophages is pronounced plasticity, which manifests itself in the ability of macrophages to change their functional phenotype depending on the micromilieu. Apoptotic cell clearance (efferocytosis) is an important inducer of a macrophage polarization to M2 phenotype under pathological settings. Previously, we have developed an original protocol for the generation of M2-like macrophages, polarized by efferocytosis under serum-deprived conditions (M2 (LS), Low Serum). The present study was aimed to assess a neuroregenerative potential of M2 (LS) macrophages. We studied their effect on the differentiation of SH-SY5Y cells in comparison with retinoic acid (RA). As the morphological criteria of differentiation we have assessed the relative content of differentiated cells, i.e., cells with a neurite length exceeding the cell body length, and the average neurite length on days 3, 7, and 13. The ratio of neuron-like (N-type) and epithelial-like (S-type) cells in cultures was also assessed. SH-SY5Y cells were characterized by a low level of spontaneous differentiation, both under standard conditions (10% FBS) and serum deprivation (1% FBS). Upon RA treatment, SH-SY5Y cells stopped proliferating and underwent neuronal differentiation. Cultivation of SH-SY5Y cells in the presence of M2 (LS) conditioned medium also led to a significant increase in the relative content of differentiated cells, the average length of neurite-like processes, as well as a change in the balance of S- and N-type cells towards a pronounced predominance of the latter. The morphological features of differentiation were significantly less pronounced at early stage (day 3) of differentiation as compared with the RA-induced changes and reached the level of positive control only at later stages (day 13) (p < 0.05). In contrast to retinoic acid, M2 (LS) conditioned medium induced neuronal differentiation of SH-SY5Y cells without suppressing their proliferative activity. The data obtained may indicate a high neuroregenerative potential of M2 macrophages in vitro, which is realized through soluble factors and manifests itself in promoting SH-SY5Y differentiation.I. M. RashchupkinE. Ya. ShevelaE. R. ChernykhSPb RAACIarticlemacrophagesm2 phenotypesh-sy5y cellsdifferentiationretinoic acidneuroregenerationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 677-684 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	macrophages m2 phenotype sh-sy5y cells differentiation retinoic acid neuroregeneration Immunologic diseases. Allergy RC581-607
spellingShingle	macrophages m2 phenotype sh-sy5y cells differentiation retinoic acid neuroregeneration Immunologic diseases. Allergy RC581-607 I. M. Rashchupkin E. Ya. Shevela E. R. Chernykh EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
description	Macrophages play a key role in triggering and regulation of neuroregeneration. The characteristic feature of macrophages is pronounced plasticity, which manifests itself in the ability of macrophages to change their functional phenotype depending on the micromilieu. Apoptotic cell clearance (efferocytosis) is an important inducer of a macrophage polarization to M2 phenotype under pathological settings. Previously, we have developed an original protocol for the generation of M2-like macrophages, polarized by efferocytosis under serum-deprived conditions (M2 (LS), Low Serum). The present study was aimed to assess a neuroregenerative potential of M2 (LS) macrophages. We studied their effect on the differentiation of SH-SY5Y cells in comparison with retinoic acid (RA). As the morphological criteria of differentiation we have assessed the relative content of differentiated cells, i.e., cells with a neurite length exceeding the cell body length, and the average neurite length on days 3, 7, and 13. The ratio of neuron-like (N-type) and epithelial-like (S-type) cells in cultures was also assessed. SH-SY5Y cells were characterized by a low level of spontaneous differentiation, both under standard conditions (10% FBS) and serum deprivation (1% FBS). Upon RA treatment, SH-SY5Y cells stopped proliferating and underwent neuronal differentiation. Cultivation of SH-SY5Y cells in the presence of M2 (LS) conditioned medium also led to a significant increase in the relative content of differentiated cells, the average length of neurite-like processes, as well as a change in the balance of S- and N-type cells towards a pronounced predominance of the latter. The morphological features of differentiation were significantly less pronounced at early stage (day 3) of differentiation as compared with the RA-induced changes and reached the level of positive control only at later stages (day 13) (p < 0.05). In contrast to retinoic acid, M2 (LS) conditioned medium induced neuronal differentiation of SH-SY5Y cells without suppressing their proliferative activity. The data obtained may indicate a high neuroregenerative potential of M2 macrophages in vitro, which is realized through soluble factors and manifests itself in promoting SH-SY5Y differentiation.
format	article
author	I. M. Rashchupkin E. Ya. Shevela E. R. Chernykh
author_facet	I. M. Rashchupkin E. Ya. Shevela E. R. Chernykh
author_sort	I. M. Rashchupkin
title	EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
title_short	EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
title_full	EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
title_fullStr	EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
title_full_unstemmed	EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS
title_sort	effect of m2 macrophage-derived soluble factors on differentiation of sh-sy5y cells
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/6415eacb459e485a861d532dd7a8f240
work_keys_str_mv	AT imrashchupkin effectofm2macrophagederivedsolublefactorsondifferentiationofshsy5ycells AT eyashevela effectofm2macrophagederivedsolublefactorsondifferentiationofshsy5ycells AT erchernykh effectofm2macrophagederivedsolublefactorsondifferentiationofshsy5ycells
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EFFECT OF M2 MACROPHAGE-DERIVED SOLUBLE FACTORS ON DIFFERENTIATION OF SH-SY5Y CELLS

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