Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism

Abstract Approximately two thirds of freshwater mussel species in the United States and Canada are imperiled, and populations are declining rapidly. Translocation and captive management are commonly used to mitigate losses of freshwater mussel biodiversity, but these conservation tools may result in...

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Autores principales:	Ieva Roznere, Brandon T. Sinn, Marymegan Daly, G. Thomas Watters
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/64277c36d0ea4be9a06e0541df7a14a1
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spelling	oai:doaj.org-article:64277c36d0ea4be9a06e0541df7a14a12021-12-02T14:16:57ZFreshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism10.1038/s41598-021-81856-72045-2322https://doaj.org/article/64277c36d0ea4be9a06e0541df7a14a12021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81856-7https://doaj.org/toc/2045-2322Abstract Approximately two thirds of freshwater mussel species in the United States and Canada are imperiled, and populations are declining rapidly. Translocation and captive management are commonly used to mitigate losses of freshwater mussel biodiversity, but these conservation tools may result in decreased growth and increased mortality. This study uses RNA-Seq to determine how translocation into captivity affects gene expression in Amblema plicata. Mussels were collected from the Muskingum River in Ohio, USA and brought into a captive holding facility. RNA was extracted from gill tissue 11 months post translocation from mussels in captivity and the Muskingum River on the same day. RNA was sequenced on an Illumina HiSeq 2500, and differential expression analysis was performed on de novo assembled transcripts. More than 1200 transcripts were up-regulated in captive mussels, and 246 were assigned functional annotations. Many up-regulated transcripts were involved in energy metabolism and the stress response, such as heat shock proteins and antioxidants. More than 500 transcripts were down-regulated in captive mussels, and 41 were assigned functional annotations. We observed an over-representation of down-regulated transcripts associated with immune response. Our work suggests that A. plicata experienced moderate levels of stress and altered energy metabolism and immune response for at least 11 months post translocation into captivity.Ieva RoznereBrandon T. SinnMarymegan DalyG. Thomas WattersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ieva Roznere Brandon T. Sinn Marymegan Daly G. Thomas Watters Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
description	Abstract Approximately two thirds of freshwater mussel species in the United States and Canada are imperiled, and populations are declining rapidly. Translocation and captive management are commonly used to mitigate losses of freshwater mussel biodiversity, but these conservation tools may result in decreased growth and increased mortality. This study uses RNA-Seq to determine how translocation into captivity affects gene expression in Amblema plicata. Mussels were collected from the Muskingum River in Ohio, USA and brought into a captive holding facility. RNA was extracted from gill tissue 11 months post translocation from mussels in captivity and the Muskingum River on the same day. RNA was sequenced on an Illumina HiSeq 2500, and differential expression analysis was performed on de novo assembled transcripts. More than 1200 transcripts were up-regulated in captive mussels, and 246 were assigned functional annotations. Many up-regulated transcripts were involved in energy metabolism and the stress response, such as heat shock proteins and antioxidants. More than 500 transcripts were down-regulated in captive mussels, and 41 were assigned functional annotations. We observed an over-representation of down-regulated transcripts associated with immune response. Our work suggests that A. plicata experienced moderate levels of stress and altered energy metabolism and immune response for at least 11 months post translocation into captivity.
format	article
author	Ieva Roznere Brandon T. Sinn Marymegan Daly G. Thomas Watters
author_facet	Ieva Roznere Brandon T. Sinn Marymegan Daly G. Thomas Watters
author_sort	Ieva Roznere
title	Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
title_short	Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
title_full	Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
title_fullStr	Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
title_full_unstemmed	Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
title_sort	freshwater mussels (unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/64277c36d0ea4be9a06e0541df7a14a1
work_keys_str_mv	AT ievaroznere freshwatermusselsunionidaebroughtintocaptivityexhibitupregulationofgenesinvolvedinstressandenergymetabolism AT brandontsinn freshwatermusselsunionidaebroughtintocaptivityexhibitupregulationofgenesinvolvedinstressandenergymetabolism AT marymegandaly freshwatermusselsunionidaebroughtintocaptivityexhibitupregulationofgenesinvolvedinstressandenergymetabolism AT gthomaswatters freshwatermusselsunionidaebroughtintocaptivityexhibitupregulationofgenesinvolvedinstressandenergymetabolism
_version_	1718391669689155584

Freshwater mussels (Unionidae) brought into captivity exhibit up-regulation of genes involved in stress and energy metabolism

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