GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA

The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expr...

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Autores principales:	V. N. Mineev, L. N. Sorokina, M. A. Nyoma, V. I. Trofimov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	bronchial asthma th2-cytokines gata-3 lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/642f7e243e204c67b9daa8c04ac8b806
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spelling	oai:doaj.org-article:642f7e243e204c67b9daa8c04ac8b8062021-11-18T08:03:37ZGATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA1563-06252313-741X10.15789/1563-0625-2010-1-2-21-28https://doaj.org/article/642f7e243e204c67b9daa8c04ac8b8062014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/35https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham). An antibody against GATA-3 (Abcam, UK) was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA). Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic diseaseV. N. MineevL. N. SorokinaM. A. NyomaV. I. TrofimovSPb RAACIarticlebronchial asthmath2-cytokinesgata-3lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 1-2, Pp 21-28 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial asthma th2-cytokines gata-3 lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial asthma th2-cytokines gata-3 lymphocytes Immunologic diseases. Allergy RC581-607 V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
description	The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham). An antibody against GATA-3 (Abcam, UK) was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA). Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic disease
format	article
author	V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov
author_facet	V. N. Mineev L. N. Sorokina M. A. Nyoma V. I. Trofimov
author_sort	V. N. Mineev
title	GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
title_short	GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
title_full	GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
title_fullStr	GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
title_full_unstemmed	GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
title_sort	gata-3 expression in peripheral blood lymphocytes of patients with bronchial asthma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/642f7e243e204c67b9daa8c04ac8b806
work_keys_str_mv	AT vnmineev gata3expressioninperipheralbloodlymphocytesofpatientswithbronchialasthma AT lnsorokina gata3expressioninperipheralbloodlymphocytesofpatientswithbronchialasthma AT manyoma gata3expressioninperipheralbloodlymphocytesofpatientswithbronchialasthma AT vitrofimov gata3expressioninperipheralbloodlymphocytesofpatientswithbronchialasthma
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GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA

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