Detection and survival of Candida albicans in soils

Abstract Candida albicans is the most common human fungal pathogen. This yeast is described as a commensal of human and animal mucosa, and very few studies have focused on its isolation in natural environments. We investigated the presence of C. albicans in a large panel of French soils. Because a c...

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Autores principales:	Marc Sautour, Jean‐Paul Lemaître, Lionel Ranjard, Caroline Truntzer, Louise Basmaciyan, Géraldine Depret, Alain Hartmann, Frédéric Dalle
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Candida albicans cation exchange capacity detection nested PCR pH soil Environmental sciences GE1-350 Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/642fd61e02de457f81c116ab538a6027
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spelling	oai:doaj.org-article:642fd61e02de457f81c116ab538a60272021-11-23T16:15:24ZDetection and survival of Candida albicans in soils2637-494310.1002/edn3.230https://doaj.org/article/642fd61e02de457f81c116ab538a60272021-11-01T00:00:00Zhttps://doi.org/10.1002/edn3.230https://doaj.org/toc/2637-4943Abstract Candida albicans is the most common human fungal pathogen. This yeast is described as a commensal of human and animal mucosa, and very few studies have focused on its isolation in natural environments. We investigated the presence of C. albicans in a large panel of French soils. Because a culture‐based method didn't allow isolation of the yeast in a panel of 70 soils, we adapted a nested‐PCR for detecting C. albicans DNA in a panel of 460 soils. Only 7 of the 460 soil samples (1.5%) were PCR‐positive for Candida albicans. To understand which parameters influence the survival of the yeast, we studied the decline of a population of C. albicans over a period of one month in a collection of 20 soils collected throughout France. C. albicans was able to survive up to 30 days in 80% of the soils tested. Using a Spearman correlation test, we showed that the short‐term survival of C. albicans in soils was correlated with some soils chemical factors such as pH and presence of some minerals (Al, Mn, and Na). Concerning survival after 30 days, Cation Exchange Capacity (CEC) and clay content were identified as beneficial determinants of the long‐term survival of C. albicans in soils.Marc SautourJean‐Paul LemaîtreLionel RanjardCaroline TruntzerLouise BasmaciyanGéraldine DepretAlain HartmannFrédéric DalleWileyarticleCandida albicanscation exchange capacitydetectionnested PCRpHsoilEnvironmental sciencesGE1-350Microbial ecologyQR100-130ENEnvironmental DNA, Vol 3, Iss 6, Pp 1093-1101 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans cation exchange capacity detection nested PCR pH soil Environmental sciences GE1-350 Microbial ecology QR100-130
spellingShingle	Candida albicans cation exchange capacity detection nested PCR pH soil Environmental sciences GE1-350 Microbial ecology QR100-130 Marc Sautour Jean‐Paul Lemaître Lionel Ranjard Caroline Truntzer Louise Basmaciyan Géraldine Depret Alain Hartmann Frédéric Dalle Detection and survival of Candida albicans in soils
description	Abstract Candida albicans is the most common human fungal pathogen. This yeast is described as a commensal of human and animal mucosa, and very few studies have focused on its isolation in natural environments. We investigated the presence of C. albicans in a large panel of French soils. Because a culture‐based method didn't allow isolation of the yeast in a panel of 70 soils, we adapted a nested‐PCR for detecting C. albicans DNA in a panel of 460 soils. Only 7 of the 460 soil samples (1.5%) were PCR‐positive for Candida albicans. To understand which parameters influence the survival of the yeast, we studied the decline of a population of C. albicans over a period of one month in a collection of 20 soils collected throughout France. C. albicans was able to survive up to 30 days in 80% of the soils tested. Using a Spearman correlation test, we showed that the short‐term survival of C. albicans in soils was correlated with some soils chemical factors such as pH and presence of some minerals (Al, Mn, and Na). Concerning survival after 30 days, Cation Exchange Capacity (CEC) and clay content were identified as beneficial determinants of the long‐term survival of C. albicans in soils.
format	article
author	Marc Sautour Jean‐Paul Lemaître Lionel Ranjard Caroline Truntzer Louise Basmaciyan Géraldine Depret Alain Hartmann Frédéric Dalle
author_facet	Marc Sautour Jean‐Paul Lemaître Lionel Ranjard Caroline Truntzer Louise Basmaciyan Géraldine Depret Alain Hartmann Frédéric Dalle
author_sort	Marc Sautour
title	Detection and survival of Candida albicans in soils
title_short	Detection and survival of Candida albicans in soils
title_full	Detection and survival of Candida albicans in soils
title_fullStr	Detection and survival of Candida albicans in soils
title_full_unstemmed	Detection and survival of Candida albicans in soils
title_sort	detection and survival of candida albicans in soils
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/642fd61e02de457f81c116ab538a6027
work_keys_str_mv	AT marcsautour detectionandsurvivalofcandidaalbicansinsoils AT jeanpaullemaitre detectionandsurvivalofcandidaalbicansinsoils AT lionelranjard detectionandsurvivalofcandidaalbicansinsoils AT carolinetruntzer detectionandsurvivalofcandidaalbicansinsoils AT louisebasmaciyan detectionandsurvivalofcandidaalbicansinsoils AT geraldinedepret detectionandsurvivalofcandidaalbicansinsoils AT alainhartmann detectionandsurvivalofcandidaalbicansinsoils AT fredericdalle detectionandsurvivalofcandidaalbicansinsoils
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Detection and survival of Candida albicans in soils

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