Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction

Abstract Background Implantation of a biomaterial may induce the foreign-body reaction to the host tissue that determines the outcome of the integration and the biological performance of the implants. The foreign-body reaction can be modulated by control of the material properties of the implants. M...

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Autores principales:	Jiaxi Chen, Huiqun Zhou, Daping Xie, Yiming Niu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bletilla striata polysaccharide Cryogel Spatial regulation Foreign-body reaction Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/64351420bca04ca49bef678775c880f6
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spelling	oai:doaj.org-article:64351420bca04ca49bef678775c880f62021-12-05T12:07:06ZBletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction10.1186/s13020-021-00526-y1749-8546https://doaj.org/article/64351420bca04ca49bef678775c880f62021-12-01T00:00:00Zhttps://doi.org/10.1186/s13020-021-00526-yhttps://doaj.org/toc/1749-8546Abstract Background Implantation of a biomaterial may induce the foreign-body reaction to the host tissue that determines the outcome of the integration and the biological performance of the implants. The foreign-body reaction can be modulated by control of the material properties of the implants. Methods First, we synthesized methacrylated Bletilla striata Polysaccharide (BSP-MA) and constructed a series of open porous cryogels utilizing this material via the freezing-thawing treatment of solvent-precursors systems. Second, Pore size and modulus were measured to characterize the properties of BSP cryogels. Live/dead staining of cells and CCK-8 were performed to test the cytocompatibility of the scaffolds. In addition, the Real-Time qPCR experiments were carried for the tests. Finally, the BSP scaffolds were implanted subcutaneously to verify the foreign-body reaction between host tissue and materials. Results Our data demonstrated that cryogels with different pore sizes and modulus can be fabricated by just adjusting the concentration. Besides, the cryogels showed well cytocompatibility in the in vitro experiments and exhibited upregulated expression levels of pro-inflammation-related genes (Tnfa and Il1b) with the increase of pore size. In vivo experiments further proved that with the increase of pore size, more immune cells infiltrated into the inner zone of materials. The foreign-body reaction and the distribution of immune-regulatory cells could be modulated by tuning the material microstructure. Conclusions Collectively, our findings revealed Bletilla striata polysaccharide cryogel scaffold with different pore sizes can spatially control foreign-body reaction. The microstructure of cryogels could differentially guide the distribution of inflammatory cells, affect the formation of blood vessels and fibrous capsules, which eventually influence the material-tissue integration. This work demonstrates a practical strategy to regulate foreign body reaction and promote the performance of medical devices.Jiaxi ChenHuiqun ZhouDaping XieYiming NiuBMCarticleBletilla striata polysaccharideCryogelSpatial regulationForeign-body reactionOther systems of medicineRZ201-999ENChinese Medicine, Vol 16, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bletilla striata polysaccharide Cryogel Spatial regulation Foreign-body reaction Other systems of medicine RZ201-999
spellingShingle	Bletilla striata polysaccharide Cryogel Spatial regulation Foreign-body reaction Other systems of medicine RZ201-999 Jiaxi Chen Huiqun Zhou Daping Xie Yiming Niu Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
description	Abstract Background Implantation of a biomaterial may induce the foreign-body reaction to the host tissue that determines the outcome of the integration and the biological performance of the implants. The foreign-body reaction can be modulated by control of the material properties of the implants. Methods First, we synthesized methacrylated Bletilla striata Polysaccharide (BSP-MA) and constructed a series of open porous cryogels utilizing this material via the freezing-thawing treatment of solvent-precursors systems. Second, Pore size and modulus were measured to characterize the properties of BSP cryogels. Live/dead staining of cells and CCK-8 were performed to test the cytocompatibility of the scaffolds. In addition, the Real-Time qPCR experiments were carried for the tests. Finally, the BSP scaffolds were implanted subcutaneously to verify the foreign-body reaction between host tissue and materials. Results Our data demonstrated that cryogels with different pore sizes and modulus can be fabricated by just adjusting the concentration. Besides, the cryogels showed well cytocompatibility in the in vitro experiments and exhibited upregulated expression levels of pro-inflammation-related genes (Tnfa and Il1b) with the increase of pore size. In vivo experiments further proved that with the increase of pore size, more immune cells infiltrated into the inner zone of materials. The foreign-body reaction and the distribution of immune-regulatory cells could be modulated by tuning the material microstructure. Conclusions Collectively, our findings revealed Bletilla striata polysaccharide cryogel scaffold with different pore sizes can spatially control foreign-body reaction. The microstructure of cryogels could differentially guide the distribution of inflammatory cells, affect the formation of blood vessels and fibrous capsules, which eventually influence the material-tissue integration. This work demonstrates a practical strategy to regulate foreign body reaction and promote the performance of medical devices.
format	article
author	Jiaxi Chen Huiqun Zhou Daping Xie Yiming Niu
author_facet	Jiaxi Chen Huiqun Zhou Daping Xie Yiming Niu
author_sort	Jiaxi Chen
title	Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
title_short	Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
title_full	Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
title_fullStr	Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
title_full_unstemmed	Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
title_sort	bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction
publisher	BMC
publishDate	2021
url	https://doaj.org/article/64351420bca04ca49bef678775c880f6
work_keys_str_mv	AT jiaxichen bletillastriatapolysaccharidecryogelscaffoldforspatialcontrolofforeignbodyreaction AT huiqunzhou bletillastriatapolysaccharidecryogelscaffoldforspatialcontrolofforeignbodyreaction AT dapingxie bletillastriatapolysaccharidecryogelscaffoldforspatialcontrolofforeignbodyreaction AT yimingniu bletillastriatapolysaccharidecryogelscaffoldforspatialcontrolofforeignbodyreaction
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Bletilla striata polysaccharide cryogel scaffold for spatial control of foreign-body reaction

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