Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers

The objective of the experiment was to investigate the effects of dietary supplementation with 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester (HMBi) on the nitrogen (N) metabolism in beef steers. The plasma metabolites analyzed by metabolome profiling were used to clarify the impact mechanis...

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Autores principales:	Yuchao Zhao, Md Sazzadur Rahman, Mengmeng Li, Guangyong Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cattle methionine hydroxy analog nitrogen retention plasma metabolome profiling Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/643555cf999b4804ad9c3559430f61c0
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spelling	oai:doaj.org-article:643555cf999b4804ad9c3559430f61c02021-11-25T16:21:12ZEffects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers10.3390/ani111133112076-2615https://doaj.org/article/643555cf999b4804ad9c3559430f61c02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3311https://doaj.org/toc/2076-2615The objective of the experiment was to investigate the effects of dietary supplementation with 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester (HMBi) on the nitrogen (N) metabolism in beef steers. The plasma metabolites analyzed by metabolome profiling were used to clarify the impact mechanism. Three Simmental steers (body weight, 593 ± 23 kg) were used as experimental animals. Three levels of HMBi (i.e., 0, 12, and 24 g d<sup>−1</sup>) were added in a basal ration as experimental treatments. The steers and the dietary treatments were randomly allocated in a 3 × 3 Latin square design. The results showed that supplementing HMBi up to 24 g d<sup>−1</sup> did not affect the N retention and N retention rate (NRR), and the fecal N/urinary N ratio even though it tended to linearly increase the uric acid N/urinary N ratio in steers. The results of plasma metabolome profiling showed that supplementing HMBi at 24 g d<sup>−1</sup> upregulated the plasma concentrations of L-methionine (Met); Met-related metabolites including betaine, Met sulfoxide, and taurine; and L-isoleucine and tyrosine, whereas it downregulated L-serine, glycine, diaminopimelic acid, and other metabolites. The reason for the nonsignificant effect of HMBi on improving the N utilization in steers could be that the steers used in the experiment were in the fattening period. It is suggested to evaluate the effects of the dietary addition of HMBi using growing cattle in further research.Yuchao ZhaoMd Sazzadur RahmanMengmeng LiGuangyong ZhaoMDPI AGarticlecattlemethionine hydroxy analognitrogen retentionplasma metabolome profilingVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3311, p 3311 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cattle methionine hydroxy analog nitrogen retention plasma metabolome profiling Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	cattle methionine hydroxy analog nitrogen retention plasma metabolome profiling Veterinary medicine SF600-1100 Zoology QL1-991 Yuchao Zhao Md Sazzadur Rahman Mengmeng Li Guangyong Zhao Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
description	The objective of the experiment was to investigate the effects of dietary supplementation with 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester (HMBi) on the nitrogen (N) metabolism in beef steers. The plasma metabolites analyzed by metabolome profiling were used to clarify the impact mechanism. Three Simmental steers (body weight, 593 ± 23 kg) were used as experimental animals. Three levels of HMBi (i.e., 0, 12, and 24 g d<sup>−1</sup>) were added in a basal ration as experimental treatments. The steers and the dietary treatments were randomly allocated in a 3 × 3 Latin square design. The results showed that supplementing HMBi up to 24 g d<sup>−1</sup> did not affect the N retention and N retention rate (NRR), and the fecal N/urinary N ratio even though it tended to linearly increase the uric acid N/urinary N ratio in steers. The results of plasma metabolome profiling showed that supplementing HMBi at 24 g d<sup>−1</sup> upregulated the plasma concentrations of L-methionine (Met); Met-related metabolites including betaine, Met sulfoxide, and taurine; and L-isoleucine and tyrosine, whereas it downregulated L-serine, glycine, diaminopimelic acid, and other metabolites. The reason for the nonsignificant effect of HMBi on improving the N utilization in steers could be that the steers used in the experiment were in the fattening period. It is suggested to evaluate the effects of the dietary addition of HMBi using growing cattle in further research.
format	article
author	Yuchao Zhao Md Sazzadur Rahman Mengmeng Li Guangyong Zhao
author_facet	Yuchao Zhao Md Sazzadur Rahman Mengmeng Li Guangyong Zhao
author_sort	Yuchao Zhao
title	Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
title_short	Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
title_full	Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
title_fullStr	Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
title_full_unstemmed	Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers
title_sort	effects of dietary supplementation with 2-hydroxy-4-(methylthio)-butanoic acid isopropyl ester as a methionine supplement on nitrogen utilization in steers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/643555cf999b4804ad9c3559430f61c0
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Effects of Dietary Supplementation with 2-Hydroxy-4-(methylthio)-butanoic Acid Isopropyl Ester as a Methionine Supplement on Nitrogen Utilization in Steers

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