Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination

A total number of 27 vaccine vials including 3 batches of 3 different types of live attenuated poultry viral vaccines (tissue culture Gumboro vaccine, egg adapted colored ILT vaccine and egg adapted non colored ILT vaccine) were experimentally contaminated with 1,5,10 CFU of Salmonella typhimurium r...

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Autores principales:	Hanan M. Ibrahim, Hanan A. Ahmed, Nourhan Nagy, Gina M. Mohamed, Shafai S.M., Aly A.M.
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2019
Materias:	bacterial contamination detection limits live attenuated vaccines salmonella Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2019.62668 https://doaj.org/article/64367fd0d08a4b1caa50c220598db3c6
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spelling	oai:doaj.org-article:64367fd0d08a4b1caa50c220598db3c62021-12-02T13:21:43ZDetection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contaminationhttps://dx.doi.org/10.21608/javs.2019.626681687-40722090-3308https://doaj.org/article/64367fd0d08a4b1caa50c220598db3c62019-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62668.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308A total number of 27 vaccine vials including 3 batches of 3 different types of live attenuated poultry viral vaccines (tissue culture Gumboro vaccine, egg adapted colored ILT vaccine and egg adapted non colored ILT vaccine) were experimentally contaminated with 1,5,10 CFU of Salmonella typhimurium reference strain/dose then subjected for sterility testing as referenced in OIE. Further dilution was carried out of vaccines showing negative results. The results of statical analysis showed that there is a significant difference between direct inoculation of the vaccines and their dilutions. It could be concluded that the applied testing method is appropriate for testing sterility of living attenuated poultry viral vaccines but testing of some of such vaccines need further dilutions to obtain accurate results.Hanan M. IbrahimHanan A. AhmedNourhan NagyGina M. MohamedShafai S.M.Aly A.M.Egyptian Society for Animal Managementarticlebacterial contaminationdetection limitslive attenuated vaccinessalmonellaZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 4, Iss 1, Pp 1-4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial contamination detection limits live attenuated vaccines salmonella Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	bacterial contamination detection limits live attenuated vaccines salmonella Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Hanan M. Ibrahim Hanan A. Ahmed Nourhan Nagy Gina M. Mohamed Shafai S.M. Aly A.M. Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
description	A total number of 27 vaccine vials including 3 batches of 3 different types of live attenuated poultry viral vaccines (tissue culture Gumboro vaccine, egg adapted colored ILT vaccine and egg adapted non colored ILT vaccine) were experimentally contaminated with 1,5,10 CFU of Salmonella typhimurium reference strain/dose then subjected for sterility testing as referenced in OIE. Further dilution was carried out of vaccines showing negative results. The results of statical analysis showed that there is a significant difference between direct inoculation of the vaccines and their dilutions. It could be concluded that the applied testing method is appropriate for testing sterility of living attenuated poultry viral vaccines but testing of some of such vaccines need further dilutions to obtain accurate results.
format	article
author	Hanan M. Ibrahim Hanan A. Ahmed Nourhan Nagy Gina M. Mohamed Shafai S.M. Aly A.M.
author_facet	Hanan M. Ibrahim Hanan A. Ahmed Nourhan Nagy Gina M. Mohamed Shafai S.M. Aly A.M.
author_sort	Hanan M. Ibrahim
title	Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
title_short	Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
title_full	Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
title_fullStr	Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
title_full_unstemmed	Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination
title_sort	detection limits of live attenuated poultry viral vaccine testing method for detection of bacterial contamination
publisher	Egyptian Society for Animal Management
publishDate	2019
url	https://dx.doi.org/10.21608/javs.2019.62668 https://doaj.org/article/64367fd0d08a4b1caa50c220598db3c6
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Detection Limits Of Live Attenuated Poultry Viral Vaccine Testing Method For Detection Of Bacterial Contamination

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