Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

Abstract Antibiotic resistance poses one of the greatest threats to global health today; conventional drug therapies are becoming increasingly inefficacious and limited. We identified 16 medicinal plant species used by traditional healers for the treatment of infectious and inflammatory diseases in...

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Autores principales:	Fabien Schultz, Godwin Anywar, Huaqiao Tang, François Chassagne, James T. Lyles, Leif-Alexander Garbe, Cassandra L. Quave
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/644cd8eae24945c5b0a551c9508ba234
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spelling	oai:doaj.org-article:644cd8eae24945c5b0a551c9508ba2342021-12-02T16:26:37ZTargeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda10.1038/s41598-020-67572-82045-2322https://doaj.org/article/644cd8eae24945c5b0a551c9508ba2342020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67572-8https://doaj.org/toc/2045-2322Abstract Antibiotic resistance poses one of the greatest threats to global health today; conventional drug therapies are becoming increasingly inefficacious and limited. We identified 16 medicinal plant species used by traditional healers for the treatment of infectious and inflammatory diseases in the Greater Mpigi region of Uganda. Extracts were evaluated for their ability to inhibit growth of clinical isolates of multidrug-resistant ESKAPE pathogens. Extracts were also screened for quorum quenching activity against S. aureus, including direct protein output assessment (δ-toxin), and cytotoxicity against human keratinocytes (HaCaT). Putative matches of compounds were elucidated via LC–FTMS for the best-performing extracts. These were extracts of Zanthoxylum chalybeum (Staphylococcus aureus: MIC: 16 μg/mL; Enterococcus faecium: MIC: 32 μg/mL) and Harungana madagascariensis (S. aureus: MIC: 32 μg/mL; E. faecium: MIC: 32 μg/mL) stem bark. Extracts of Solanum aculeastrum root bark and Sesamum calycinum subsp. angustifolium leaves exhibited strong quorum sensing inhibition activity against all S. aureus accessory gene regulator (agr) alleles in absence of growth inhibition (IC50 values: 1–64 μg/mL). The study provided scientific evidence for the potential therapeutic efficacy of these medicinal plants in the Greater Mpigi region used for infections and wounds, with 13 out of 16 species tested being validated with in vitro studies.Fabien SchultzGodwin AnywarHuaqiao TangFrançois ChassagneJames T. LylesLeif-Alexander GarbeCassandra L. QuaveNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-19 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabien Schultz Godwin Anywar Huaqiao Tang François Chassagne James T. Lyles Leif-Alexander Garbe Cassandra L. Quave Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
description	Abstract Antibiotic resistance poses one of the greatest threats to global health today; conventional drug therapies are becoming increasingly inefficacious and limited. We identified 16 medicinal plant species used by traditional healers for the treatment of infectious and inflammatory diseases in the Greater Mpigi region of Uganda. Extracts were evaluated for their ability to inhibit growth of clinical isolates of multidrug-resistant ESKAPE pathogens. Extracts were also screened for quorum quenching activity against S. aureus, including direct protein output assessment (δ-toxin), and cytotoxicity against human keratinocytes (HaCaT). Putative matches of compounds were elucidated via LC–FTMS for the best-performing extracts. These were extracts of Zanthoxylum chalybeum (Staphylococcus aureus: MIC: 16 μg/mL; Enterococcus faecium: MIC: 32 μg/mL) and Harungana madagascariensis (S. aureus: MIC: 32 μg/mL; E. faecium: MIC: 32 μg/mL) stem bark. Extracts of Solanum aculeastrum root bark and Sesamum calycinum subsp. angustifolium leaves exhibited strong quorum sensing inhibition activity against all S. aureus accessory gene regulator (agr) alleles in absence of growth inhibition (IC50 values: 1–64 μg/mL). The study provided scientific evidence for the potential therapeutic efficacy of these medicinal plants in the Greater Mpigi region used for infections and wounds, with 13 out of 16 species tested being validated with in vitro studies.
format	article
author	Fabien Schultz Godwin Anywar Huaqiao Tang François Chassagne James T. Lyles Leif-Alexander Garbe Cassandra L. Quave
author_facet	Fabien Schultz Godwin Anywar Huaqiao Tang François Chassagne James T. Lyles Leif-Alexander Garbe Cassandra L. Quave
author_sort	Fabien Schultz
title	Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
title_short	Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
title_full	Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
title_fullStr	Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
title_full_unstemmed	Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda
title_sort	targeting eskape pathogens with anti-infective medicinal plants from the greater mpigi region in uganda
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/644cd8eae24945c5b0a551c9508ba234
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Targeting ESKAPE pathogens with anti-infective medicinal plants from the Greater Mpigi region in Uganda

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