In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)

An investigation on in vitro plant regeneration of endangered medicinal plant Rauvolfia serpentina L. was carried out. The newly emerging leaves were used as explants, which were transferred to half strength MS medium along with various combinations of growth regulators for callus regeneration. The...

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Autores principales:	Subhendu S Gantait, Koushik Dutta, Jayoti Majumder
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2017
Materias:	in vitro regeneration rauvolfia callus medicinal plant Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/64574be27d544902b3c323e88cdec90a
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spelling	oai:doaj.org-article:64574be27d544902b3c323e88cdec90a2021-12-02T10:22:31ZIn vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)0973-354X2582-4899https://doaj.org/article/64574be27d544902b3c323e88cdec90a2017-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/74https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899An investigation on in vitro plant regeneration of endangered medicinal plant Rauvolfia serpentina L. was carried out. The newly emerging leaves were used as explants, which were transferred to half strength MS medium along with various combinations of growth regulators for callus regeneration. The half strength MS medium fortified with 5.0 mgl-1 2,4-D and 2.0 mgl-1 NAA was found most suitable for qualitative (light green colour) and quantitative callus production (86%). The shoot regeneration (81.67 %) and elongation (5.67 cm) was highest in full MS medium supplemented with 6.0 mgl-1 BAP and 2.0 mgl- 1 GA3 and consequently, the root initiation was highest (55%) in half strength MS medium containing 3.0 mgl-1 IBA. The regenerated plantlets were preserved for nine months in good condition through repeated subculturing on full strength MS media. The callus were kept in storage by repeated sub-culturing in in vitro condition on MS medium fortified with 0.2 mgl-1 2,4-D and the preservation could be extended for nine months in its best condition. It was evident that, the regeneration capacity of callus was reduced as the time of callus storage was increased.Subhendu S GantaitKoushik DuttaJayoti MajumderSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlein vitroregenerationrauvolfiacallusmedicinal plantPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 12, Iss 1, Pp 71-77 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	in vitro regeneration rauvolfia callus medicinal plant Plant culture SB1-1110
spellingShingle	in vitro regeneration rauvolfia callus medicinal plant Plant culture SB1-1110 Subhendu S Gantait Koushik Dutta Jayoti Majumder In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
description	An investigation on in vitro plant regeneration of endangered medicinal plant Rauvolfia serpentina L. was carried out. The newly emerging leaves were used as explants, which were transferred to half strength MS medium along with various combinations of growth regulators for callus regeneration. The half strength MS medium fortified with 5.0 mgl-1 2,4-D and 2.0 mgl-1 NAA was found most suitable for qualitative (light green colour) and quantitative callus production (86%). The shoot regeneration (81.67 %) and elongation (5.67 cm) was highest in full MS medium supplemented with 6.0 mgl-1 BAP and 2.0 mgl- 1 GA3 and consequently, the root initiation was highest (55%) in half strength MS medium containing 3.0 mgl-1 IBA. The regenerated plantlets were preserved for nine months in good condition through repeated subculturing on full strength MS media. The callus were kept in storage by repeated sub-culturing in in vitro condition on MS medium fortified with 0.2 mgl-1 2,4-D and the preservation could be extended for nine months in its best condition. It was evident that, the regeneration capacity of callus was reduced as the time of callus storage was increased.
format	article
author	Subhendu S Gantait Koushik Dutta Jayoti Majumder
author_facet	Subhendu S Gantait Koushik Dutta Jayoti Majumder
author_sort	Subhendu S Gantait
title	In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
title_short	In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
title_full	In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
title_fullStr	In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
title_full_unstemmed	In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)
title_sort	in vitro regeneration and conservation of an endangered medicinal plant sarpagandha (rauvolfia serpentina l.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2017
url	https://doaj.org/article/64574be27d544902b3c323e88cdec90a
work_keys_str_mv	AT subhendusgantait invitroregenerationandconservationofanendangeredmedicinalplantsarpagandharauvolfiaserpentinal AT koushikdutta invitroregenerationandconservationofanendangeredmedicinalplantsarpagandharauvolfiaserpentinal AT jayotimajumder invitroregenerationandconservationofanendangeredmedicinalplantsarpagandharauvolfiaserpentinal
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In vitro regeneration and conservation of an endangered medicinal plant sarpagandha (Rauvolfia serpentina L.)

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