Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves

Abstract Bovine Respiratory Syncytial Virus (BRSV) is a primary viral cause of Bovine Respiratory Disease (BRD) in young calves, which is responsible for substantial morbidity and mortality. Infection with BRSV induces global gene expression changes in respiratory tissues. If these changes are obser...

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Autores principales:	Dayle Johnston, Bernadette Earley, Matthew S. McCabe, JaeWoo Kim, Jeremy F. Taylor, Ken Lemon, Catherine Duffy, Michael McMenamy, S. Louise Cosby, Sinead M. Waters
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/645bacb3c3e14f39b727b5edb3e28c89
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spelling	oai:doaj.org-article:645bacb3c3e14f39b727b5edb3e28c892021-12-02T16:56:09ZMessenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves10.1038/s41598-021-88878-12045-2322https://doaj.org/article/645bacb3c3e14f39b727b5edb3e28c892021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88878-1https://doaj.org/toc/2045-2322Abstract Bovine Respiratory Syncytial Virus (BRSV) is a primary viral cause of Bovine Respiratory Disease (BRD) in young calves, which is responsible for substantial morbidity and mortality. Infection with BRSV induces global gene expression changes in respiratory tissues. If these changes are observed in tissues which are more accessible in live animals, such as whole blood, they may be used as biomarkers for diagnosis of the disease. Therefore, the objective of the current study was to elucidate the whole blood transcriptomic response of dairy calves to an experimental challenge with BRSV. Calves (Holstein–Friesian) were either administered BRSV inoculate (103.5 TCID50/ml × 15 ml) (n = 12) or sterile phosphate buffered saline (n = 6). Clinical signs were scored daily and whole blood was collected in Tempus RNA tubes immediately prior to euthanasia, at day 7 post-challenge. RNA was extracted from blood and sequenced (150 bp paired-end). The sequence reads were aligned to the bovine reference genome (UMD3.1) and EdgeR was subsequently employed for differential gene expression analysis. Multidimensional scaling showed that samples from BRSV challenged and control calves segregated based on whole blood gene expression changes, despite the BRSV challenged calves only displaying mild clinical symptoms of the disease. There were 281 differentially expressed (DE) genes (p < 0.05, FDR < 0.1, fold change > 2) between the BRSV challenged and control calves. The top enriched KEGG pathways and gene ontology terms were associated with viral infection and included “Influenza A”, “defense response to virus”, “regulation of viral life cycle” and “innate immune response”. Highly DE genes involved in these pathways may be beneficial for the diagnosis of subclinical BRD from blood samples.Dayle JohnstonBernadette EarleyMatthew S. McCabeJaeWoo KimJeremy F. TaylorKen LemonCatherine DuffyMichael McMenamyS. Louise CosbySinead M. WatersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dayle Johnston Bernadette Earley Matthew S. McCabe JaeWoo Kim Jeremy F. Taylor Ken Lemon Catherine Duffy Michael McMenamy S. Louise Cosby Sinead M. Waters Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
description	Abstract Bovine Respiratory Syncytial Virus (BRSV) is a primary viral cause of Bovine Respiratory Disease (BRD) in young calves, which is responsible for substantial morbidity and mortality. Infection with BRSV induces global gene expression changes in respiratory tissues. If these changes are observed in tissues which are more accessible in live animals, such as whole blood, they may be used as biomarkers for diagnosis of the disease. Therefore, the objective of the current study was to elucidate the whole blood transcriptomic response of dairy calves to an experimental challenge with BRSV. Calves (Holstein–Friesian) were either administered BRSV inoculate (103.5 TCID50/ml × 15 ml) (n = 12) or sterile phosphate buffered saline (n = 6). Clinical signs were scored daily and whole blood was collected in Tempus RNA tubes immediately prior to euthanasia, at day 7 post-challenge. RNA was extracted from blood and sequenced (150 bp paired-end). The sequence reads were aligned to the bovine reference genome (UMD3.1) and EdgeR was subsequently employed for differential gene expression analysis. Multidimensional scaling showed that samples from BRSV challenged and control calves segregated based on whole blood gene expression changes, despite the BRSV challenged calves only displaying mild clinical symptoms of the disease. There were 281 differentially expressed (DE) genes (p < 0.05, FDR < 0.1, fold change > 2) between the BRSV challenged and control calves. The top enriched KEGG pathways and gene ontology terms were associated with viral infection and included “Influenza A”, “defense response to virus”, “regulation of viral life cycle” and “innate immune response”. Highly DE genes involved in these pathways may be beneficial for the diagnosis of subclinical BRD from blood samples.
format	article
author	Dayle Johnston Bernadette Earley Matthew S. McCabe JaeWoo Kim Jeremy F. Taylor Ken Lemon Catherine Duffy Michael McMenamy S. Louise Cosby Sinead M. Waters
author_facet	Dayle Johnston Bernadette Earley Matthew S. McCabe JaeWoo Kim Jeremy F. Taylor Ken Lemon Catherine Duffy Michael McMenamy S. Louise Cosby Sinead M. Waters
author_sort	Dayle Johnston
title	Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
title_short	Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
title_full	Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
title_fullStr	Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
title_full_unstemmed	Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves
title_sort	messenger rna biomarkers of bovine respiratory syncytial virus infection in the whole blood of dairy calves
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/645bacb3c3e14f39b727b5edb3e28c89
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Messenger RNA biomarkers of Bovine Respiratory Syncytial Virus infection in the whole blood of dairy calves

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