Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>

ABSTRACT A major challenge facing bacterial intestinal pathogens is competition for nutrient sources with the host microbiota. Vibrio cholerae is an intestinal pathogen that causes cholera, which affects millions each year; however, our knowledge of its nutritional requirements in the intestinal mil...

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Autores principales:	Nathan D. McDonald, Jean-Bernard Lubin, Nityananda Chowdhury, E. Fidelma Boyd
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/645db41820df4967b4e93162237d5101
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spelling	oai:doaj.org-article:645db41820df4967b4e93162237d51012021-11-15T15:41:41ZHost-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>10.1128/mBio.02237-152150-7511https://doaj.org/article/645db41820df4967b4e93162237d51012016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02237-15https://doaj.org/toc/2150-7511ABSTRACT A major challenge facing bacterial intestinal pathogens is competition for nutrient sources with the host microbiota. Vibrio cholerae is an intestinal pathogen that causes cholera, which affects millions each year; however, our knowledge of its nutritional requirements in the intestinal milieu is limited. In this study, we demonstrated that V. cholerae can grow efficiently on intestinal mucus and its component sialic acids and that a tripartite ATP-independent periplasmic SiaPQM strain, transporter-deficient mutant NC1777, was attenuated for colonization using a streptomycin-pretreated adult mouse model. In in vivo competition assays, NC1777 was significantly outcompeted for up to 3 days postinfection. NC1777 was also significantly outcompeted in in vitro competition assays in M9 minimal medium supplemented with intestinal mucus, indicating that sialic acid uptake is essential for fitness. Phylogenetic analyses demonstrated that the ability to utilize sialic acid was distributed among 452 bacterial species from eight phyla. The majority of species belonged to four phyla, Actinobacteria (members of Actinobacillus, Corynebacterium, Mycoplasma, and Streptomyces), Bacteroidetes (mainly Bacteroides, Capnocytophaga, and Prevotella), Firmicutes (members of Streptococcus, Staphylococcus, Clostridium, and Lactobacillus), and Proteobacteria (including Escherichia, Shigella, Salmonella, Citrobacter, Haemophilus, Klebsiella, Pasteurella, Photobacterium, Vibrio, and Yersinia species), mostly commensals and/or pathogens. Overall, our data demonstrate that the ability to take up host-derived sugars and sialic acid specifically allows V. cholerae a competitive advantage in intestinal colonization and that this is a trait that is sporadic in its occurrence and phylogenetic distribution and ancestral in some genera but horizontally acquired in others. IMPORTANCE Sialic acids are nine carbon amino sugars that are abundant on all mucous surfaces. The deadly human pathogen Vibrio cholerae contains the genes required for scavenging, transport, and catabolism of sialic acid. We determined that the V. cholerae SiaPQM transporter is essential for sialic acid transport and that this trait allows the bacterium to outcompete noncatabolizers in vivo. We also showed that the ability to take up and catabolize sialic acid is prevalent among both commensals and pathogens that colonize the oral cavity and the respiratory, intestinal, and urogenital tracts. Phylogenetic analysis determined that the sialic acid catabolism phenotype is ancestral in some genera such as Yersinia, Streptococcus, and Staphylococcus and is acquired by horizontal gene transfer in others such as Vibrio, Aeromonas, and Klebsiella. The data demonstrate that this trait has evolved multiple times in different lineages, indicating the importance of specialized metabolism to niche expansion.Nathan D. McDonaldJean-Bernard LubinNityananda ChowdhuryE. Fidelma BoydAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Nathan D. McDonald Jean-Bernard Lubin Nityananda Chowdhury E. Fidelma Boyd Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
description	ABSTRACT A major challenge facing bacterial intestinal pathogens is competition for nutrient sources with the host microbiota. Vibrio cholerae is an intestinal pathogen that causes cholera, which affects millions each year; however, our knowledge of its nutritional requirements in the intestinal milieu is limited. In this study, we demonstrated that V. cholerae can grow efficiently on intestinal mucus and its component sialic acids and that a tripartite ATP-independent periplasmic SiaPQM strain, transporter-deficient mutant NC1777, was attenuated for colonization using a streptomycin-pretreated adult mouse model. In in vivo competition assays, NC1777 was significantly outcompeted for up to 3 days postinfection. NC1777 was also significantly outcompeted in in vitro competition assays in M9 minimal medium supplemented with intestinal mucus, indicating that sialic acid uptake is essential for fitness. Phylogenetic analyses demonstrated that the ability to utilize sialic acid was distributed among 452 bacterial species from eight phyla. The majority of species belonged to four phyla, Actinobacteria (members of Actinobacillus, Corynebacterium, Mycoplasma, and Streptomyces), Bacteroidetes (mainly Bacteroides, Capnocytophaga, and Prevotella), Firmicutes (members of Streptococcus, Staphylococcus, Clostridium, and Lactobacillus), and Proteobacteria (including Escherichia, Shigella, Salmonella, Citrobacter, Haemophilus, Klebsiella, Pasteurella, Photobacterium, Vibrio, and Yersinia species), mostly commensals and/or pathogens. Overall, our data demonstrate that the ability to take up host-derived sugars and sialic acid specifically allows V. cholerae a competitive advantage in intestinal colonization and that this is a trait that is sporadic in its occurrence and phylogenetic distribution and ancestral in some genera but horizontally acquired in others. IMPORTANCE Sialic acids are nine carbon amino sugars that are abundant on all mucous surfaces. The deadly human pathogen Vibrio cholerae contains the genes required for scavenging, transport, and catabolism of sialic acid. We determined that the V. cholerae SiaPQM transporter is essential for sialic acid transport and that this trait allows the bacterium to outcompete noncatabolizers in vivo. We also showed that the ability to take up and catabolize sialic acid is prevalent among both commensals and pathogens that colonize the oral cavity and the respiratory, intestinal, and urogenital tracts. Phylogenetic analysis determined that the sialic acid catabolism phenotype is ancestral in some genera such as Yersinia, Streptococcus, and Staphylococcus and is acquired by horizontal gene transfer in others such as Vibrio, Aeromonas, and Klebsiella. The data demonstrate that this trait has evolved multiple times in different lineages, indicating the importance of specialized metabolism to niche expansion.
format	article
author	Nathan D. McDonald Jean-Bernard Lubin Nityananda Chowdhury E. Fidelma Boyd
author_facet	Nathan D. McDonald Jean-Bernard Lubin Nityananda Chowdhury E. Fidelma Boyd
author_sort	Nathan D. McDonald
title	Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
title_short	Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
title_full	Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
title_fullStr	Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
title_full_unstemmed	Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>
title_sort	host-derived sialic acids are an important nutrient source required for optimal bacterial fitness <italic toggle="yes">in vivo</italic>
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/645db41820df4967b4e93162237d5101
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Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness <italic toggle="yes">In Vivo</italic>

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