Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization

ABSTRACT Enterococcus faecalis is paradoxically a dangerous nosocomial pathogen and a normal constituent of the human gut microbiome, an environment rich in ethanolamine. E. faecalis carries the eut (ethanolamine utilization) genes, which enable the catabolism of ethanolamine (EA) as a valuable sour...

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Autores principales:	Karan Gautam Kaval, Kavindra V. Singh, Melissa R. Cruz, Sruti DebRoy, Wade C. Winkler, Barbara E. Murray, Danielle A. Garsin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Enterococcus ethanolamine intestinal colonization Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/64653fa2c8f942b09a46ef17c1a58a57
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spelling	oai:doaj.org-article:64653fa2c8f942b09a46ef17c1a58a572021-11-15T16:00:27ZLoss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization10.1128/mBio.00790-182150-7511https://doaj.org/article/64653fa2c8f942b09a46ef17c1a58a572018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00790-18https://doaj.org/toc/2150-7511ABSTRACT Enterococcus faecalis is paradoxically a dangerous nosocomial pathogen and a normal constituent of the human gut microbiome, an environment rich in ethanolamine. E. faecalis carries the eut (ethanolamine utilization) genes, which enable the catabolism of ethanolamine (EA) as a valuable source of carbon and/or nitrogen. EA catabolism was previously shown to contribute to the colonization and growth of enteric pathogens, such as Salmonella enterica serovar Typhimurium and enterohemorrhagic Escherichia coli (EHEC), in the gut environment. We tested the ability of eut mutants of E. faecalis to colonize the gut using a murine model of gastrointestinal (GI) tract competition and report the surprising observation that these mutants outcompete the wild-type strain. IMPORTANCE Some bacteria that are normal, harmless colonizers of the human body can cause disease in immunocompromised patients, particularly those that have been heavily treated with antibiotics. Therefore, it is important to understand the factors that promote or negate these organisms’ ability to colonize. Previously, ethanolamine, found in high concentrations in the GI tract, was shown to promote the colonization and growth of bacteria associated with food poisoning. Here, we report the surprising, opposite effect of ethanolamine utilization on the commensal colonizer E. faecalis, namely, that loss of this metabolic capacity made it a better colonizer.Karan Gautam KavalKavindra V. SinghMelissa R. CruzSruti DebRoyWade C. WinklerBarbara E. MurrayDanielle A. GarsinAmerican Society for MicrobiologyarticleEnterococcusethanolamineintestinal colonizationMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Enterococcus ethanolamine intestinal colonization Microbiology QR1-502
spellingShingle	Enterococcus ethanolamine intestinal colonization Microbiology QR1-502 Karan Gautam Kaval Kavindra V. Singh Melissa R. Cruz Sruti DebRoy Wade C. Winkler Barbara E. Murray Danielle A. Garsin Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
description	ABSTRACT Enterococcus faecalis is paradoxically a dangerous nosocomial pathogen and a normal constituent of the human gut microbiome, an environment rich in ethanolamine. E. faecalis carries the eut (ethanolamine utilization) genes, which enable the catabolism of ethanolamine (EA) as a valuable source of carbon and/or nitrogen. EA catabolism was previously shown to contribute to the colonization and growth of enteric pathogens, such as Salmonella enterica serovar Typhimurium and enterohemorrhagic Escherichia coli (EHEC), in the gut environment. We tested the ability of eut mutants of E. faecalis to colonize the gut using a murine model of gastrointestinal (GI) tract competition and report the surprising observation that these mutants outcompete the wild-type strain. IMPORTANCE Some bacteria that are normal, harmless colonizers of the human body can cause disease in immunocompromised patients, particularly those that have been heavily treated with antibiotics. Therefore, it is important to understand the factors that promote or negate these organisms’ ability to colonize. Previously, ethanolamine, found in high concentrations in the GI tract, was shown to promote the colonization and growth of bacteria associated with food poisoning. Here, we report the surprising, opposite effect of ethanolamine utilization on the commensal colonizer E. faecalis, namely, that loss of this metabolic capacity made it a better colonizer.
format	article
author	Karan Gautam Kaval Kavindra V. Singh Melissa R. Cruz Sruti DebRoy Wade C. Winkler Barbara E. Murray Danielle A. Garsin
author_facet	Karan Gautam Kaval Kavindra V. Singh Melissa R. Cruz Sruti DebRoy Wade C. Winkler Barbara E. Murray Danielle A. Garsin
author_sort	Karan Gautam Kaval
title	Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
title_short	Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
title_full	Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
title_fullStr	Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
title_full_unstemmed	Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization
title_sort	loss of ethanolamine utilization in <named-content content-type="genus-species">enterococcus faecalis</named-content> increases gastrointestinal tract colonization
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/64653fa2c8f942b09a46ef17c1a58a57
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Loss of Ethanolamine Utilization in <named-content content-type="genus-species">Enterococcus faecalis</named-content> Increases Gastrointestinal Tract Colonization

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