PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

Abstract We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed r...

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Autores principales:	B. Molina-Moya, A. Lacoma, N. García-Sierra, S. Blanco, L. Haba, S. Samper, J. Ruiz-Manzano, C. Prat, C. Arnold, J. Domínguez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6466a499d7dc4df1a59d0b07e28432f0
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spelling	oai:doaj.org-article:6466a499d7dc4df1a59d0b07e28432f02021-12-02T16:06:48ZPyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping10.1038/s41598-017-06760-52045-2322https://doaj.org/article/6466a499d7dc4df1a59d0b07e28432f02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06760-5https://doaj.org/toc/2045-2322Abstract We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units – variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.B. Molina-MoyaA. LacomaN. García-SierraS. BlancoL. HabaS. SamperJ. Ruiz-ManzanoC. PratC. ArnoldJ. DomínguezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B. Molina-Moya A. Lacoma N. García-Sierra S. Blanco L. Haba S. Samper J. Ruiz-Manzano C. Prat C. Arnold J. Domínguez PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
description	Abstract We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units – variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.
format	article
author	B. Molina-Moya A. Lacoma N. García-Sierra S. Blanco L. Haba S. Samper J. Ruiz-Manzano C. Prat C. Arnold J. Domínguez
author_facet	B. Molina-Moya A. Lacoma N. García-Sierra S. Blanco L. Haba S. Samper J. Ruiz-Manzano C. Prat C. Arnold J. Domínguez
author_sort	B. Molina-Moya
title	PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_short	PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_full	PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_fullStr	PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_full_unstemmed	PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping
title_sort	pyrotyping, a novel pyrosequencing-based assay for mycobacterium tuberculosis genotyping
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6466a499d7dc4df1a59d0b07e28432f0
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PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

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