CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3

Abstract Background Natural killer/T-cell lymphoma (NKTCL) is a rare and aggressive subtype of Non-Hodgkin’s Lymphoma. CircRNA has shown great potential to become a biomarker in plasma. In this study, we aimed to determine circRNA for its diagnostic and prognostic value and biological function in NK...

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Autores principales:	Mei Mei, Yingjun Wang, Wenting Song, Zhaoming Li, Qilong Wang, Jiayin Li, Mingzhi Zhang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Noncoding RNAs Circular RNAs microRNAs Natural killer/T-cell lymphoma (NKTCL) Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/646e19fb4583441da7222d7613c19060
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spelling	oai:doaj.org-article:646e19fb4583441da7222d7613c190602021-11-08T11:13:42ZCircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat310.1186/s12935-021-02296-x1475-2867https://doaj.org/article/646e19fb4583441da7222d7613c190602021-11-01T00:00:00Zhttps://doi.org/10.1186/s12935-021-02296-xhttps://doaj.org/toc/1475-2867Abstract Background Natural killer/T-cell lymphoma (NKTCL) is a rare and aggressive subtype of Non-Hodgkin’s Lymphoma. CircRNA has shown great potential to become a biomarker in plasma. In this study, we aimed to determine circRNA for its diagnostic and prognostic value and biological function in NKTCL. Method The circRNA microarray of plasma from NKTCL patients and healthy donors were conducted. The relative expressions of target circRNA were verified by qRT-PCR. We conducted function experiments in vitro and in vivo. Bioinformatics predicted the target miRNA of the target circRNA and the binding site was detected by the dual luciferase report assay. Downstream target protein was predicted and detected by western blot in vitro and immunohistochemistry in vivo. Result By analyzing the plasma circRNA microarrays in NKTCL, 6137 circRNAs were up-regulated and 6190 circRNAs were down-regulated. The relative expressions of circADARB1 were significantly higher in NKTCL patients. The knockdown of circADARB1 inhibited proliferation of NKTCL cells in vitro and in vivo. CircADARB1 could bind to miR-214-3p in the downstream and regulate the expression of p-Stat3. In nude mice tumor tissue, p-Stat3 was under-expressed in the circADARB1 knockdown group. Conclusion CircADARB1 was highly expressed in NKTCL plasma and circADARB1 was a potential biomarker to assist diagnosis and predict the response in NKTCL. CircADARB1 bound up to miR-214-3p and regulated p-Stat3.Mei MeiYingjun WangWenting SongZhaoming LiQilong WangJiayin LiMingzhi ZhangBMCarticleNoncoding RNAsCircular RNAsmicroRNAsNatural killer/T-cell lymphoma (NKTCL)Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCancer Cell International, Vol 21, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Noncoding RNAs Circular RNAs microRNAs Natural killer/T-cell lymphoma (NKTCL) Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Noncoding RNAs Circular RNAs microRNAs Natural killer/T-cell lymphoma (NKTCL) Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Mei Mei Yingjun Wang Wenting Song Zhaoming Li Qilong Wang Jiayin Li Mingzhi Zhang CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
description	Abstract Background Natural killer/T-cell lymphoma (NKTCL) is a rare and aggressive subtype of Non-Hodgkin’s Lymphoma. CircRNA has shown great potential to become a biomarker in plasma. In this study, we aimed to determine circRNA for its diagnostic and prognostic value and biological function in NKTCL. Method The circRNA microarray of plasma from NKTCL patients and healthy donors were conducted. The relative expressions of target circRNA were verified by qRT-PCR. We conducted function experiments in vitro and in vivo. Bioinformatics predicted the target miRNA of the target circRNA and the binding site was detected by the dual luciferase report assay. Downstream target protein was predicted and detected by western blot in vitro and immunohistochemistry in vivo. Result By analyzing the plasma circRNA microarrays in NKTCL, 6137 circRNAs were up-regulated and 6190 circRNAs were down-regulated. The relative expressions of circADARB1 were significantly higher in NKTCL patients. The knockdown of circADARB1 inhibited proliferation of NKTCL cells in vitro and in vivo. CircADARB1 could bind to miR-214-3p in the downstream and regulate the expression of p-Stat3. In nude mice tumor tissue, p-Stat3 was under-expressed in the circADARB1 knockdown group. Conclusion CircADARB1 was highly expressed in NKTCL plasma and circADARB1 was a potential biomarker to assist diagnosis and predict the response in NKTCL. CircADARB1 bound up to miR-214-3p and regulated p-Stat3.
format	article
author	Mei Mei Yingjun Wang Wenting Song Zhaoming Li Qilong Wang Jiayin Li Mingzhi Zhang
author_facet	Mei Mei Yingjun Wang Wenting Song Zhaoming Li Qilong Wang Jiayin Li Mingzhi Zhang
author_sort	Mei Mei
title	CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
title_short	CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
title_full	CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
title_fullStr	CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
title_full_unstemmed	CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3
title_sort	circadarb1 serves as a new biomarker in natural killer t-cell lymphoma and a potential regulator of p-stat3
publisher	BMC
publishDate	2021
url	https://doaj.org/article/646e19fb4583441da7222d7613c19060
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CircADARB1 serves as a new biomarker in natural killer T-cell lymphoma and a potential regulator of p-Stat3

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