Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.

Bacterial cells are mostly studied during planktonic growth although in their natural habitats they are often found in communities such as biofilms with dramatically different physiological properties. We have examined another type of community namely cellular aggregates observed in strains of the h...

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Autores principales:	Jakob Haaber, Marianne Thorup Cohn, Dorte Frees, Thorbjørn Joest Andersen, Hanne Ingmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6475b8bf526946aaba559f6178799642
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spelling	oai:doaj.org-article:6475b8bf526946aaba559f61787996422021-11-18T07:12:00ZPlanktonic aggregates of Staphylococcus aureus protect against common antibiotics.1932-620310.1371/journal.pone.0041075https://doaj.org/article/6475b8bf526946aaba559f61787996422012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22815921/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial cells are mostly studied during planktonic growth although in their natural habitats they are often found in communities such as biofilms with dramatically different physiological properties. We have examined another type of community namely cellular aggregates observed in strains of the human pathogen Staphylococcus aureus. By laser-diffraction particle-size analysis (LDA) we show, for strains forming visible aggregates, that the aggregation starts already in the early exponential growth phase and proceeds until post-exponential phase where more than 90% of the population is part of the aggregate community. Similar to some types of biofilm, the structural component of S. aureus aggregates is the polysaccharide intercellular adhesin (PIA). Importantly, PIA production correlates with the level of aggregation whether altered through mutations or exposure to sub-inhibitory concentrations of selected antibiotics. While some properties of aggregates resemble those of biofilms including increased mutation frequency and survival during antibiotic treatment, aggregated cells displayed higher metabolic activity than planktonic cells or cells in biofilm. Thus, our data indicate that the properties of cells in aggregates differ in some aspects from those in biofilms. It is generally accepted that the biofilm life style protects pathogens against antibiotics and the hostile environment of the host. We speculate that in aggregate communities S. aureus increases its tolerance to hazardous environments and that the combination of a biofilm-like environment with mobility has substantial practical and clinical importance.Jakob HaaberMarianne Thorup CohnDorte FreesThorbjørn Joest AndersenHanne IngmerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41075 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jakob Haaber Marianne Thorup Cohn Dorte Frees Thorbjørn Joest Andersen Hanne Ingmer Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
description	Bacterial cells are mostly studied during planktonic growth although in their natural habitats they are often found in communities such as biofilms with dramatically different physiological properties. We have examined another type of community namely cellular aggregates observed in strains of the human pathogen Staphylococcus aureus. By laser-diffraction particle-size analysis (LDA) we show, for strains forming visible aggregates, that the aggregation starts already in the early exponential growth phase and proceeds until post-exponential phase where more than 90% of the population is part of the aggregate community. Similar to some types of biofilm, the structural component of S. aureus aggregates is the polysaccharide intercellular adhesin (PIA). Importantly, PIA production correlates with the level of aggregation whether altered through mutations or exposure to sub-inhibitory concentrations of selected antibiotics. While some properties of aggregates resemble those of biofilms including increased mutation frequency and survival during antibiotic treatment, aggregated cells displayed higher metabolic activity than planktonic cells or cells in biofilm. Thus, our data indicate that the properties of cells in aggregates differ in some aspects from those in biofilms. It is generally accepted that the biofilm life style protects pathogens against antibiotics and the hostile environment of the host. We speculate that in aggregate communities S. aureus increases its tolerance to hazardous environments and that the combination of a biofilm-like environment with mobility has substantial practical and clinical importance.
format	article
author	Jakob Haaber Marianne Thorup Cohn Dorte Frees Thorbjørn Joest Andersen Hanne Ingmer
author_facet	Jakob Haaber Marianne Thorup Cohn Dorte Frees Thorbjørn Joest Andersen Hanne Ingmer
author_sort	Jakob Haaber
title	Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
title_short	Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
title_full	Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
title_fullStr	Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
title_full_unstemmed	Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.
title_sort	planktonic aggregates of staphylococcus aureus protect against common antibiotics.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6475b8bf526946aaba559f6178799642
work_keys_str_mv	AT jakobhaaber planktonicaggregatesofstaphylococcusaureusprotectagainstcommonantibiotics AT mariannethorupcohn planktonicaggregatesofstaphylococcusaureusprotectagainstcommonantibiotics AT dortefrees planktonicaggregatesofstaphylococcusaureusprotectagainstcommonantibiotics AT thorbjørnjoestandersen planktonicaggregatesofstaphylococcusaureusprotectagainstcommonantibiotics AT hanneingmer planktonicaggregatesofstaphylococcusaureusprotectagainstcommonantibiotics
_version_	1718423791374172160

Planktonic aggregates of Staphylococcus aureus protect against common antibiotics.

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