Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro

Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation...

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Autores principales:	Vasiliki Zoi, Vasiliki Galani, Evrysthenis Vartholomatos, Natalia Zacharopoulou, Eftichia Tsoumeleka, Georgios Gkizas, Georgios Bozios, Pericles Tsekeris, Ieremias Chousidis, Ioannis Leonardos, Andreas G. Tzakos, Athanasios P. Kyritsis, George A. Alexiou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	curcumin glioblastoma cells radiation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/647864cbaf384089830a4e5ade7c45c9
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spelling	oai:doaj.org-article:647864cbaf384089830a4e5ade7c45c92021-11-25T16:49:03ZCurcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro10.3390/biomedicines91115622227-9059https://doaj.org/article/647864cbaf384089830a4e5ade7c45c92021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1562https://doaj.org/toc/2227-9059Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation, and the combined effect was compared to the antiproliferative effect of each agent when given individually. Cell viability and proliferation were evaluated with the trypan blue exclusion assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic effects of the combination treatment were analyzed with CompuSyn software. To examine how the co-treatment affected different phases of cell-cycle progression, a cell-cycle analysis via flow cytometry was performed. Treatment with curcumin and radiation significantly reduced cell viability in both U87 and T98 cell lines. The combination treatment arrested both cell lines at the G2/M phase to a higher extent than radiation or curcumin treatment alone. The synergistic effect of curcumin when combined with temozolomide resulted in increased tumor cell death. Our results demonstrate for the first time that low doses of curcumin and irradiation exhibit a strong synergistic anti-proliferative effect on glioblastoma cells in vitro. Therefore, this combination may represent an innovative and promising strategy for the treatment of glioblastoma, and further studies are needed to fully understand the molecular mechanism underlying this effect.Vasiliki ZoiVasiliki GalaniEvrysthenis VartholomatosNatalia ZacharopoulouEftichia TsoumelekaGeorgios GkizasGeorgios BoziosPericles TsekerisIeremias ChousidisIoannis LeonardosAndreas G. TzakosAthanasios P. KyritsisGeorge A. AlexiouMDPI AGarticlecurcuminglioblastoma cellsradiationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1562, p 1562 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	curcumin glioblastoma cells radiation Biology (General) QH301-705.5
spellingShingle	curcumin glioblastoma cells radiation Biology (General) QH301-705.5 Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
description	Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation, and the combined effect was compared to the antiproliferative effect of each agent when given individually. Cell viability and proliferation were evaluated with the trypan blue exclusion assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic effects of the combination treatment were analyzed with CompuSyn software. To examine how the co-treatment affected different phases of cell-cycle progression, a cell-cycle analysis via flow cytometry was performed. Treatment with curcumin and radiation significantly reduced cell viability in both U87 and T98 cell lines. The combination treatment arrested both cell lines at the G2/M phase to a higher extent than radiation or curcumin treatment alone. The synergistic effect of curcumin when combined with temozolomide resulted in increased tumor cell death. Our results demonstrate for the first time that low doses of curcumin and irradiation exhibit a strong synergistic anti-proliferative effect on glioblastoma cells in vitro. Therefore, this combination may represent an innovative and promising strategy for the treatment of glioblastoma, and further studies are needed to fully understand the molecular mechanism underlying this effect.
format	article
author	Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou
author_facet	Vasiliki Zoi Vasiliki Galani Evrysthenis Vartholomatos Natalia Zacharopoulou Eftichia Tsoumeleka Georgios Gkizas Georgios Bozios Pericles Tsekeris Ieremias Chousidis Ioannis Leonardos Andreas G. Tzakos Athanasios P. Kyritsis George A. Alexiou
author_sort	Vasiliki Zoi
title	Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
title_short	Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
title_full	Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
title_fullStr	Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
title_full_unstemmed	Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro
title_sort	curcumin and radiotherapy exert synergistic anti-glioma effect in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/647864cbaf384089830a4e5ade7c45c9
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Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro

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