Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.

As cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanyan Zhao, Qian Xiong, Ping Xie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/647876c5ed9b46f69f9c78c0273e8354
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:647876c5ed9b46f69f9c78c0273e8354
record_format	dspace
spelling	oai:doaj.org-article:647876c5ed9b46f69f9c78c0273e83542021-11-18T06:49:03ZAnalysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.1932-620310.1371/journal.pone.0022676https://doaj.org/article/647876c5ed9b46f69f9c78c0273e83542011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829477/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203As cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological study to integrate post-transcriptomic, proteomic and bioinformatics analysis to explore molecular mechanisms for developmental toxicity of MCs in zebrafish. After being microinjected directly into embryos, MC-RR dose-dependently decreased survival rates and increased malformation rates of embryos, causing various embryo abnormalities including loss of vascular integrity and hemorrhage. Expressions of 31 microRNAs (miRNAs) and 78 proteins were significantly affected at 72 hours post-fertilisation (hpf). Expressions of miR-430 and miR-125 families were also significantly changed. The altered expressions of miR-31 and miR-126 were likely responsible for the loss of vascular integrity. MC-RR significantly reduced the expressions of a number of proteins involved in energy metabolism, cell division, protein synthesis, cytoskeleton maintenance, response to stress and DNA replication. Bioinformatics analysis shows that several aberrantly expressed miRNAs and proteins (involved in various molecular pathways) were predicted to be potential MC-responsive miRNA-target pairs, and that their aberrant expressions should be the possible molecular mechanisms for the various developmental defects caused by MC-RR.Yanyan ZhaoQian XiongPing XiePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22676 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanyan Zhao Qian Xiong Ping Xie Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
description	As cynobacterial blooms frequently occur in fresh waters throughout the world, microcystins (MCs) have caused serious damage to both wildlife and human health. MCs are known to have developmental toxicity, however, the possible molecular mechanism is largely unknown. This is the first toxicological study to integrate post-transcriptomic, proteomic and bioinformatics analysis to explore molecular mechanisms for developmental toxicity of MCs in zebrafish. After being microinjected directly into embryos, MC-RR dose-dependently decreased survival rates and increased malformation rates of embryos, causing various embryo abnormalities including loss of vascular integrity and hemorrhage. Expressions of 31 microRNAs (miRNAs) and 78 proteins were significantly affected at 72 hours post-fertilisation (hpf). Expressions of miR-430 and miR-125 families were also significantly changed. The altered expressions of miR-31 and miR-126 were likely responsible for the loss of vascular integrity. MC-RR significantly reduced the expressions of a number of proteins involved in energy metabolism, cell division, protein synthesis, cytoskeleton maintenance, response to stress and DNA replication. Bioinformatics analysis shows that several aberrantly expressed miRNAs and proteins (involved in various molecular pathways) were predicted to be potential MC-responsive miRNA-target pairs, and that their aberrant expressions should be the possible molecular mechanisms for the various developmental defects caused by MC-RR.
format	article
author	Yanyan Zhao Qian Xiong Ping Xie
author_facet	Yanyan Zhao Qian Xiong Ping Xie
author_sort	Yanyan Zhao
title	Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
title_short	Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
title_full	Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
title_fullStr	Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
title_full_unstemmed	Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.
title_sort	analysis of microrna expression in embryonic developmental toxicity induced by mc-rr.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/647876c5ed9b46f69f9c78c0273e8354
work_keys_str_mv	AT yanyanzhao analysisofmicrornaexpressioninembryonicdevelopmentaltoxicityinducedbymcrr AT qianxiong analysisofmicrornaexpressioninembryonicdevelopmentaltoxicityinducedbymcrr AT pingxie analysisofmicrornaexpressioninembryonicdevelopmentaltoxicityinducedbymcrr
_version_	1718424354151202816

Analysis of microRNA expression in embryonic developmental toxicity induced by MC-RR.

Ejemplares similares