Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory

Identification of unknown microorganisms to the species level is an important component of a microbiology course. Modern technologies such as matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing offer a rapid species level identific...

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Autores principales:	Latifa B. Khan, Simon Swift, Tania Kamal, Hannah M. Read
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/647c730de160494a8201e1b5c74e7e69
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spelling	oai:doaj.org-article:647c730de160494a8201e1b5c74e7e692021-11-15T15:04:11ZSimulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory10.1128/jmbe.v19i2.15651935-78851935-7877https://doaj.org/article/647c730de160494a8201e1b5c74e7e692018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i2.1565https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Identification of unknown microorganisms to the species level is an important component of a microbiology course. Modern technologies such as matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing offer a rapid species level identification when compared to conventional phenotype-based methods, however they rely on a well-established taxonomy database and phenotypic assays can still play an important role in species determination. Another major limitation is the up-front cost of purchasing these modern specialized instruments and the requirement of skilled personnel to operate specialized equipment and software, which makes them unsuitable to use in the undergraduate teaching laboratory. Commercial biochemical identification systems such as the Oxoid Microbact™ GNB 12A/12E/24E kit is used for the identification of Enterobacteriaceae and other Gram negative bacteria in clinical and veterinary diagnostic laboratories, and food industries for disease control and treatment. In this article, we describe a method of reliably simulating this Microbact™ strip assay using artificial color liquids (which are affordable and easy to source) for the demonstration of phenotypic characterization of unknown Gram negative organisms while providing a safe teaching environment as no bacteria are used, familiarizing students with the concept of using Microbact™ 12A kit to identify a range of unknown Gram negative organisms.Latifa B. KhanSimon SwiftTania KamalHannah M. ReadAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Latifa B. Khan Simon Swift Tania Kamal Hannah M. Read Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
description	Identification of unknown microorganisms to the species level is an important component of a microbiology course. Modern technologies such as matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing offer a rapid species level identification when compared to conventional phenotype-based methods, however they rely on a well-established taxonomy database and phenotypic assays can still play an important role in species determination. Another major limitation is the up-front cost of purchasing these modern specialized instruments and the requirement of skilled personnel to operate specialized equipment and software, which makes them unsuitable to use in the undergraduate teaching laboratory. Commercial biochemical identification systems such as the Oxoid Microbact™ GNB 12A/12E/24E kit is used for the identification of Enterobacteriaceae and other Gram negative bacteria in clinical and veterinary diagnostic laboratories, and food industries for disease control and treatment. In this article, we describe a method of reliably simulating this Microbact™ strip assay using artificial color liquids (which are affordable and easy to source) for the demonstration of phenotypic characterization of unknown Gram negative organisms while providing a safe teaching environment as no bacteria are used, familiarizing students with the concept of using Microbact™ 12A kit to identify a range of unknown Gram negative organisms.
format	article
author	Latifa B. Khan Simon Swift Tania Kamal Hannah M. Read
author_facet	Latifa B. Khan Simon Swift Tania Kamal Hannah M. Read
author_sort	Latifa B. Khan
title	Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
title_short	Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
title_full	Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
title_fullStr	Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
title_full_unstemmed	Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory
title_sort	simulation of microbact strip assay using colored liquids to demonstrate identification of unknown gram-negative organisms in undergraduate laboratory
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/647c730de160494a8201e1b5c74e7e69
work_keys_str_mv	AT latifabkhan simulationofmicrobactstripassayusingcoloredliquidstodemonstrateidentificationofunknowngramnegativeorganismsinundergraduatelaboratory AT simonswift simulationofmicrobactstripassayusingcoloredliquidstodemonstrateidentificationofunknowngramnegativeorganismsinundergraduatelaboratory AT taniakamal simulationofmicrobactstripassayusingcoloredliquidstodemonstrateidentificationofunknowngramnegativeorganismsinundergraduatelaboratory AT hannahmread simulationofmicrobactstripassayusingcoloredliquidstodemonstrateidentificationofunknowngramnegativeorganismsinundergraduatelaboratory
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Simulation of MICROBACT Strip Assay Using Colored Liquids to Demonstrate Identification of Unknown Gram-Negative Organisms in Undergraduate Laboratory

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